Mod3D: A low-cost, flexible modular system of live-cell microscopy chambers and holders

Goss, Siobhan; Bazan, Carlos Barba; Neuman, Kaitlyn; Peng, Christina; Begeja, Nola; Suart, Celeste; Truant, Ray

doi:10.1371/journal.pone.0269345

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…Cells were seeded in 8-well ibiTreat µ-Slides (Ibidi) or Mod3D chambers [94] to ~95% confluence. Cells were washed with PBS then incubated with PBS containing 0 mM or 100 mM KBrO 3 for 30 min.…”

Section: Repair Assisted Damage Detection (Radd)mentioning

confidence: 99%

Poly ADP-Ribose Signaling is Dysregulated in Huntington Disease

Maiuri

Bazan

Harding

et al. 2022

Preprint

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Huntington disease (HD) is an autosomal dominant genetic neurodegenerative disease caused by a CAG expansion in the Huntingtin (HTT) gene, translating to an expanded polyglutamine tract in the huntingtin (HTT) protein. Age at disease onset is correlated to CAG repeat length, but varies by decades between individuals with identical repeat lengths. Genome-wide association studies link HD modification to DNA repair and mitochondrial health pathways. Recent clinical studies show elevated DNA damage in HD, even at the premanifest stage of disease. One of the major DNA repair nodes influencing neurodegenerative disease is the PARP pathway. Accumulation of poly ADP-ribose (PAR), produced by PARP1 and PARP2, has been implicated in the pathology of Alzheimers and Parkinsons diseases, as well as autosomal recessive cerebellar ataxia. We report that HD mutation carriers have lower cerebrospinal fluid PAR levels than healthy controls, starting at the premanifest stage. Patient-derived fibroblasts have reduced PARP1/2 activity and elevated DNA damage, while elevated PAR levels are only revealed upon inhibition of PAR degradation. These phenotypes are rescued by moderate huntingtin level reduction via the huntingtin-lowering splice modulator drug, LMI070 (Branaplam). As a direct mechanism, we have defined a PAR-binding motif in huntingtin, detected huntingtin complexed with PARylated proteins in human cells during stress, and localized huntingtin to mitotic chromosomes upon inhibition of PAR degradation. Direct huntingtin PAR binding was measured by fluorescence polarization and visualized by atomic force microscopy. These results provide insight into a very early molecular mechanism of HD, suggesting possible targets in HD to design early preventive therapies.

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Section: Repair Assisted Damage Detection (Radd)mentioning

confidence: 99%

Poly ADP-Ribose Signaling is Dysregulated in Huntington Disease

Maiuri

Bazan

Harding

et al. 2022

Preprint

Self Cite

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“…Its fundamental appeal lies in its ability to provide cost-effective and tailored access to microscopic observation instruments, thereby transcending traditional boundaries of research and education. This approach offers a diverse range of applications, spanning from histological observation to in vivo imaging, 1 encompassing optogenetics 2 and the dissemination of scientific knowledge. 3,4 The advent of 3D printing and the open-source ethos has played a pivotal role in the proliferation of DIY microscopy.…”

Section: Introductionmentioning

confidence: 99%

OpenMIC: a DIY twelve slides scanner microscope with evolutive and multimodal capabilities

Ain,

Mennane,

Maurin

et al. 2024

Optical Instrument Science, Technology, and Applications III

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Microscopy is essential in academic and medical research, driving the quest for cost-effective solutions. Inspired by modified 3D printers, these innovations enable the scanning of 2D and 3D samples, leveraging affordability and three-dimensional movement. In the realm of multimodal imaging, LED panels and LCD screens offer adaptable lighting options, balancing cost, ergonomics, and image quality remains a formidable challenge. Introducing OpenMIC, a groundbreaking solution seamlessly integrating multiple scanning features and multimodal lighting. Transformed from a 3D printer using a Raspberry Pi 4, a 64x64 LED array, and an optical module, it offers four lighting modalities, micrometric autofocus, focus stacking, image stitching, and automated scanning of 12 histological slides. OpenMIC is marked by its scalability, professional imaging quality, and a manufacturing cost under 4,000 euros.

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Mod3D: A low-cost, flexible modular system of live-cell microscopy chambers and holders

Cited by 2 publications

References 21 publications

Poly ADP-Ribose Signaling is Dysregulated in Huntington Disease

Poly ADP-Ribose Signaling is Dysregulated in Huntington Disease

OpenMIC: a DIY twelve slides scanner microscope with evolutive and multimodal capabilities

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