Mobilizing Endogenous Repair Through Understanding Immune Reaction With Biomaterials

Karkanitsa, Maria; Fathi, Parinaz; Ngo, Tran; Hall, Matthew D.

doi:10.3389/fbioe.2021.730938

Cited by 12 publications

(8 citation statements)

References 166 publications

(255 reference statements)

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“…After the acute inflammatory phase subsides, the macrophage population shifts to an M2 phenotype. M2 macrophages are characterized by the secretion of anti-inflammatory mediators (IL-10) and growth factors (PDGF and TGF-β) that aid in tissue healing by stabilizing angiogenesis (Karkanitsa et al, 2021;Martin and García, 2021). In our study, we observed the coexpression of M1 and M2 markers (Figure 6), which has usually been reported in FBR induced by scaffolds (Witherel et al, 2019).…”

Section: Discussionsupporting

confidence: 75%

In vivo biocompatibility testing of nanoparticle-functionalized alginate–chitosan scaffolds for tissue engineering applications

Viveros-Moreno,

Garcia-Lorenzana,

Peña-Mercado

et al. 2023

Front. Bioeng. Biotechnol.

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Background: There is a strong interest in designing new scaffolds for their potential application in tissue engineering and regenerative medicine. The incorporation of functionalization molecules can lead to the enhancement of scaffold properties, resulting in variations in scaffold compatibility. Therefore, the efficacy of the therapy could be compromised by the foreign body reaction triggered after implantation.Methods: In this study, the biocompatibilities of three scaffolds made from an alginate–chitosan combination and functionalized with gold nanoparticles (AuNp) and alginate-coated gold nanoparticles (AuNp + Alg) were evaluated in a subcutaneous implantation model in Wistar rats. Scaffolds and surrounding tissue were collected at 4-, 7- and 25-day postimplantation and processed for histological analysis and quantification of the expression of genes involved in angiogenesis, macrophage profile, and proinflammatory (IL-1β and TNFα) and anti-inflammatory (IL-4 and IL-10) cytokines.Results: Histological analysis showed a characteristic foreign body response that resolved 25 days postimplantation. The intensity of the reaction assessed through capsule thickness was similar among groups. Functionalizing the device with AuNp and AuNp + Alg decreased the expression of markers associated with cell death by apoptosis and polymorphonuclear leukocyte recruitment, suggesting increased compatibility with the host tissue. Similarly, the formation of many foreign body giant cells was prevented. Finally, an increased detection of alpha smooth muscle actin was observed, showing the angiogenic properties of the elaborated scaffolds.Conclusion: Our results show that the proposed scaffolds have improved biocompatibility and exhibit promising potential as biomaterials for elaborating tissue engineering constructs.

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Section: Discussionsupporting

confidence: 75%

In vivo biocompatibility testing of nanoparticle-functionalized alginate–chitosan scaffolds for tissue engineering applications

Viveros-Moreno,

Garcia-Lorenzana,

Peña-Mercado

et al. 2023

Front. Bioeng. Biotechnol.

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“…Not only proteins of the immune system associated with a material but also platelets activated on its surface stimulate the activation of neutrophils [ 1 , 48 ]. Platelets are best known as key players in thrombosis and hemostasis; recent data demonstrate the important immune and inflammatory roles of platelets in both health and disease.…”

Section: Discussionmentioning

confidence: 99%

Redox-Activation of Neutrophils Induced by Pericardium Scaffolds

Vlasova

Suleimanov

Mikhalchik

et al. 2022

IJMS

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Implantation of scaffolds causes a local inflammatory response whereby the early recruitment of neutrophils is of great importance not only for fighting the infection, but also for facilitating effective regeneration. We used luminol-dependent chemiluminescence, flow cytometry, ELISA, and confocal microscopy to assess the responses of neutrophils after the exposure to the scaffold-decellularized bovine pericardium (collagen type I) crosslinked with genipin (DBPG). We demonstrated that DBPG activated neutrophils in whole blood causing respiratory burst, myeloperoxidase (MPO) secretion, and formation of neutrophil extracellular trap-like structures (NETs). In addition, we studied platelets, another important player of the immediate immune host response. We found that platelets triggered redox-activation of isolated neutrophils by the pericardium scaffold, and likely participate in the NETs formation. Free radicals generated by neutrophils and hypochlorous acid produced by MPO are potent oxidizing agents which can oxidatively degrade biological structures. Understanding the mechanisms and consequences of redox activation of neutrophils by pericardium scaffolds is important for the development of new approaches to increase the efficiency of tissue regeneration.

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“…Ultimately, fibroblasts will be recruited and activated and will produce collagen, causing the formation of a fibrous capsule around the implant. This collagenous fibrous capsule will not only isolate the implanted material from the surrounding tissues but will also impair its function [ 13 , 14 , 15 , 16 , 17 ].…”

Section: The Neutrophil In the Foreign Body Response To Biomaterialsmentioning

confidence: 99%

The Role of Neutrophils in Biomaterial-Based Tissue Repair—Shifting Paradigms

Sousa

Barbosa

2023

JFB

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Tissue engineering and regenerative medicine are pursuing clinical valid solutions to repair and restore function of damaged tissues or organs. This can be achieved in different ways, either by promoting endogenous tissue repair or by using biomaterials or medical devices to replace damaged tissues. The understanding of the interactions of the immune system with biomaterials and how immune cells participate in the process of wound healing are critical for the development of successful solutions. Until recently, it was thought that neutrophils participate only in the initial steps of an acute inflammatory response with the role of eliminating pathogenic agents. However, the appreciation that upon activation the longevity of neutrophils is highly increased and the fact that neutrophils are highly plastic cells and can polarize into different phenotypes led to the discovery of new and important actions of neutrophils. In this review, we focus on the roles of neutrophils in the resolution of the inflammatory response, in biomaterial–tissue integration and in the subsequent tissue repair/regeneration. We also discuss the potential of neutrophils for biomaterial-based immunomodulation.

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Mobilizing Endogenous Repair Through Understanding Immune Reaction With Biomaterials

Cited by 12 publications

References 166 publications

In vivo biocompatibility testing of nanoparticle-functionalized alginate–chitosan scaffolds for tissue engineering applications

In vivo biocompatibility testing of nanoparticle-functionalized alginate–chitosan scaffolds for tissue engineering applications

Redox-Activation of Neutrophils Induced by Pericardium Scaffolds

The Role of Neutrophils in Biomaterial-Based Tissue Repair—Shifting Paradigms

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