Mobilization of the Tetu1 transposable element of Helianthus annuus: evidence for excision in different developmental stages

Fambrini, Marco; Pugliesi, Claudio

doi:10.1007/s10535-016-0655-x

Cited by 6 publications

(10 citation statements)

References 70 publications

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“…Evidence proved that some non-autonomous elements can be cross activated by autonomous partners belonging to different families [19]. This seems to be the case of Tetu1, which, despite being a non-autonomous element and also being similar to two elements of the A family, appears to be mobile due to the action of other TPases [32]. We isolated 39 TPase-encoding sequences from our database of sunflower CACTA elements, 10 of which show a weak signal of activation, and only one was found to be expressed more.…”

Section: Discussionmentioning

confidence: 96%

“…This CACTA alters the transcriptional activity of HaCYC2c, in which the expression extends into the inflorescence, suggesting that the insertion of the TE is an essential step to generate the Chry phenotype. In contrast, when the basal region of the bHLH TCP motif of the HaCYC2c gene is interrupted by the insertion of the incomplete CACTA transposon Tetu1, the ray flowers are transformed from zygomorphic to actinomorphic, assuming a resemblance to the disk flowers [20,21,32]. In this mutant, (turf ) the ray flowers also recover their hermaphroditic features, developing both male and female reproductive organs.…”

Section: Discussionmentioning

confidence: 99%

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On the Trail of Tetu1: Genome-Wide Discovery of CACTA Transposable Elements in Sunflower Genome

Ventimiglia

Pugliesi

Vangelisti

et al. 2020

IJMS

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Much has been said about sunflower (Helianthus annuus L.) retrotransposons, representing the majority of the sunflower’s repetitive component. By contrast, class II transposons remained poorly described within this species, as they present low sequence conservation and are mostly lacking coding domains, making the identification and characterization of these transposable elements difficult. The transposable element Tetu1, is a non-autonomous CACTA-like element that has been detected in the coding region of a CYCLOIDEA (CYC) gene of a sunflower mutant, tubular ray flower (turf). Based on our knowledge of Tetu1, the publicly available genome of sunflower was fully scanned. A combination of bioinformatics analyses led to the discovery of 707 putative CACTA sequences: 84 elements with complete ends and 623 truncated elements. A detailed characterization of the identified elements allowed further classification into three subgroups of 347 elements on the base of their terminal repeat sequences. Only 39 encode a protein similar to known transposases (TPase), with 10 TPase sequences showing signals of activation. Finally, an analysis of the proximity of CACTA transposons to sunflower genes showed that the majority of CACTA elements are close to the nearest gene, whereas a relevant fraction resides within gene-encoding sequences, likely interfering with sunflower genome functionality and organization.

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Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

On the Trail of Tetu1: Genome-Wide Discovery of CACTA Transposable Elements in Sunflower Genome

Ventimiglia

Pugliesi

Vangelisti

et al. 2020

IJMS

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“…Mobilization of CACTA elements usually occurs in both somatic and germ cells (Wicker, Guyot, Yahiaoui, & Keller, 2003). Notably, Tetu1 mobilization occurs through both germinal (Fambrini et al, 2011; Fambrini, Michelotti, & Pugliesi, 2007; Fambrini, Salvini, et al, 2014) and somatic (Fambrini & Pugliesi, 2017b) excision, likely in relation to the genetic background.…”

Section: Insertion Sites Of Tesmentioning

confidence: 99%

The plastic genome: The impact of transposable elements on gene functionality and genomic structural variations

Fambrini

Usai

Vangelisti

et al. 2020

Genesis

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Transposable elements (TEs) are DNA sequences that can change their position within genomes. TEs are present in most organisms and can be an important genomic component. Their activities are manifold: restructuring of genome size, chromosomal rearrangements, induction of gene mutations, and alteration of gene activity by insertion near or within promoters, intronic regions, or enhancer. There are several examples of mutations and other genetic variations determined by the activity of TEs, associated with the evolution of prokaryotic and eukaryotic organisms and the domestication of plants. Generally, TE mobilization occurs when the organism is subjected to stress, which can include both biotic and abiotic stresses, polyploidy conditions, and interspecific hybridizations, very common events in plants. TEs are widely distributed among organisms. TEs also play essential roles in evolution, but most of them are either dormant or inactive. This is mainly determined by epigenetic silencing mechanisms, regulatory systems, and control systems that aim to limit its proliferation. Furthermore, the host has recruited many genes originated from TEs as transcriptional regulators, especially in defense against pathogens and invasive genetic elements; this phenomenon is called molecular domestication. Therefore, TEs are responsible for horizontal gene transfer and the movement of genetic material between organisms, even phylogenetically distant, with a consequent remixing of their gene pools.

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“…; Chapman et al . ; Fambrini & Pugliesi ). Above all, a sunflower mutant named Chry santhemoides ( Chry ), Florepleno or d ou bl e‐flowered ( dbl ), is characterised by a shift from an actinomorphic to a zygomorphic‐like corolla of disk flowers (Cockerell ; Fick ; Heiser ; Fambrini et al .…”

Section: Introductionmentioning

confidence: 99%

Ligulate inflorescence of Helianthus × multiflorus, cv. Soleil d'Or, correlates with a mis‐regulation of a CYCLOIDEA gene characterised by insertion of a transposable element

et al. 2018

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Members of CYCLOIDEA (CYC)/TEOSINTE BRANCHED1 (TB1) transcription factor family are essential to control flower symmetry and inflorescence architecture. In the Helianthus annuus genome, ten CYC/TB1 genes have been identified. Studies performed on mutants recognised HaCYC2c as one of the key players controlling zygomorphism in sunflower. We identified CYC2c genes in the diploid Helianthus decapetalus (HdCYC2c) and in the interspecific hybrid Helianthus × multiflorus (H × mCYC2cA and H × mCYC2cB), a triploid (2n = 3× = 51), originated from unreduced eggs of H. decapetalus fertilised by reduced H. annuus male gametes. Phylogenetic analysis showed that HdCYC2c and H × mCYC2c were placed within a CYC2 subclade together with HaCYC2c but distinct from it. The present data showed that in H. × multiflorus the allele derived from H. annuus is deleted or highly modified. The H. × multiflorus taxon exists as radiate and ligulate inflorescence types. We analysed CYC2c expression in H. decapetalus and in the cultivar 'Soleil d'Or' of H. × multiflorus, a ligulate inflorescence type with actinomorphic corolla of disk flowers transformed into a zygomorphic ray-like corolla. In H. decapetalus, the HdCYC2c gene showed differential expression between developing flower types, being up-regulated in the corolla of ray flowers in comparison to the disk flower corolla. In H. × multiflorus, an insertion of 865 bp, which is part of a CACTA transposable element, was found in the 5'-untranslated region (5'-UTR) of H × mCYC2cB. This insertion could promote, even with epigenetic mechanisms, ectopic expression of the gene throughout the inflorescence, resulting in the observed loss of actinomorphy and originating a ligulate head.

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Mobilization of the Tetu1 transposable element of Helianthus annuus: evidence for excision in different developmental stages

Cited by 6 publications

References 70 publications

On the Trail of Tetu1: Genome-Wide Discovery of CACTA Transposable Elements in Sunflower Genome

On the Trail of Tetu1: Genome-Wide Discovery of CACTA Transposable Elements in Sunflower Genome

The plastic genome: The impact of transposable elements on gene functionality and genomic structural variations

Ligulate inflorescence of Helianthus × multiflorus, cv. Soleil d'Or, correlates with a mis‐regulation of a CYCLOIDEA gene characterised by insertion of a transposable element

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