Mobilization of intracellular Ca2+ induced by ADP and thrombin in platelets from diabetic patients with vascular complications

Abstract

It is shown that the baseline level of cytoplasmic Ca 2 § in platelets from diabetic patients is nearly 1.5 times as high as in healthy donors. The thrombin-induced increase of intracellular Ca 2* in patients with angiopathies is reliably lower than in the control, while in patients without angiopathies it is higher than that in donor platelets. The elevation of cytoplasmic Ca 2 § induced by ADP is greater in both groups of patients. No changes are found in the baseline level of intracellular Ca 2 § or in the ADP-induced concentration of Ca 2 § in platelets from diabetic patients during a 12-week course of insulin therapy. The intracellular Ca 2 § does not rise after 2 weeks of insulin treatment in platelets from diabetic patients.