Mobilization of hobo elements residing within the decapentaplegic gene complex: Suggestion of a new hybrid dysgenesis system in Drosophila melanogaster

Blackman, Ronald K.; Grimaila, R J; Macy, Margaret E.; Koehler, Dinah A.; Gelbart, William M.

doi:10.1016/0092-8674(87)90452-1

Cited by 184 publications

(127 citation statements)

References 25 publications

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“…Based on work we carried out on hobo transposase-mediated mobilizations of a preexisting single defective hobo element located within the decapentaplegic (dpp) locus in Drosophila (ref. 10 and L.R.B., M.A.C., and W.M.G., unpublished data), deletion events in which one endpoint of the deletion falls exactly at the end of a hobo element can be obtained readily through introduction of a source of hobo transposase. The interpretation of the origin of these events is a two-step process that occurs at sequential times within the divisions of a single germ line: (i) local hops of hobo are generated at a high rate after exposure to hobo transposase followed by (ii) transposase-mediated intrachromosomal recombination between the original hobo and the transposed copy residing nearby in the same orientation (10,18,19).…”

Section: Resultsmentioning

confidence: 98%

“…All initial D. melanogaster strains used for deletion generation had genetic backgrounds devoid of hobo elements (E lines) as determined by Southern analysis (10). hobo-mediated deletions were generated by using two P{wHy} insertions on chromosome 2.…”

Section: Methodsmentioning

confidence: 99%

“…An alternative approach is to inactivate gene function with deletions by exploiting the intrinsic property of certain mobile elements (such as the hobo or H element) to undergo homologous recombination with one another in the presence of a source of transposase (10,11). If the two mobile elements are in the vicinity of one another, the recombination event resolves as a deletion of the intervening genomic material.…”

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confidence: 99%

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A deletion-generator compound element allows deletion saturation analysis for genomewide phenotypic annotation

Huet

Myrick

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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With the available eukaryotic genome sequences, there are predictions of thousands of previously uncharacterized genes without known function or available mutational variant. Thus, there is an urgent need for efficient genetic tools for genomewide phenotypic analysis. Here we describe such a tool: a deletion-generator technology that exploits properties of a double transposable element to produce molecularly defined deletions at high density and with high efficiency. This double element, called P{wHy}, is composed of a ''deleter'' element hobo, bracketed by two genetic markers and inserted into a ''carrier'' P element. We have used this P{wHy} element in Drosophila melanogaster to generate sets of nested deletions of sufficient coverage to discriminate among every transcription unit within 60 kb of the starting insertion site. Because these two types of mobile elements, carrier and deleter, can be found in other species, our strategy should be applicable to phenotypic analysis in a variety of model organisms.

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Section: Resultsmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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A deletion-generator compound element allows deletion saturation analysis for genomewide phenotypic annotation

Huet

Myrick

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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“…They are well known to induce genome reorganization as a result of transposition or recombination events. Transposons of various families including Ac͞Ds have been associated with flanking deletions (1)(2)(3)(4)(5)(6)(7)(8). Hybrid element insertion (HEI) and excision of Drosophila P elements, a mechanism similar to HEI for Tam3 in Antirrhinum, and nonlinear transposition and transposition of partially replicated Ac͞Ds macrotransposons in maize create flanking deletions (4,(9)(10)(11)(12).…”

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confidence: 99%

Intrachromosomal excision of a hybrid Ds element induces large genomic deletions in Arabidopsis

Page

Köhler

Costa-Nunes

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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Transposon activity is known to cause chromosome rearrangements in the host genome. Surprisingly, extremely little is known about Dissociation (Ds)-induced chromosome rearrangements in Arabidopsis, where Ds is intensively used for insertional mutagenesis. Here, we describe three Arabidopsis mutants with reduced fertility and propose that excision of a hybrid Ds element induced a large genomic deletion flanking Ds. In the mutants anat and haumea, the deletion mechanism consists of a local Ds transposition from replicated into unreplicated DNA followed by Ds excision, where one end of the newly transposed element and one end of the Ds transposon at the donor site served as substrate for transposase. Excision of this hybrid element reminiscent of a macrotransposon leads to loss of the chromosomal piece located between the two ends, including one full Ds element and the flanking genomic sequence. This mechanism was found to be responsible for several other deletions and occurs at a genetically trackable frequency. Thus, it could be applied to efficiently generate deletions of various sizes in the vicinity of any existing Ds element present in the genome. In the mutant tons missing, a mechanism that involves endogenous repetitive sequences caused a large flanking deletion at a position unlinked to the starter locus. Our study of Ds transposition in Arabidopsis revealed previously undescribed mechanisms that lead to large genomic deletions flanking Ds elements, which may contribute to genome dynamics and evolution. genome evolution ͉ rearrangements ͉ transposition

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“…Active hobo elements are known to induce a suite of anomalies, termed hybrid dysgenesis, as are P and I elements (Kidwell, 1979). The symptoms of hobo hybrid dysgenesis include gonadal dysgenesis (GD), chromosomal aberrations, high mutation rates, and male recombination in the germ line of the hybrid progeny from a dysgenic cross (Lim, 1979;Yannopoulos et al, 1983Yannopoulos et al, , 1987Blackman et al, 1987). The hobo element was first cloned and characterized by McGinnis et al (1983).…”

Section: Introductionmentioning

confidence: 99%

Patterns of hobo elements and their effects in natural populations of Drosophila melanogaster in Japan

et al. 2006

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We studied the dynamics of hobo elements of Drosophila melanogaster in Japan with the goal of better understanding the invasion and evolution of transposons in natural populations. One hundred and twenty-six isofemale lines and 11 older stocks were tested for the presence and genetic phenotype of hobo elements. The oldest H strain, containing complete and deleted hobo elements, is Hikone-H (1957), but Hikone-R (1952) has no hobo-homologous sequences. The findings suggest that the hobo element invaded Japanese populations in the mid-1950s, at about the same time as the P element invasion in Japan. This chronology is consistent with the hypothesis of a recent worldwide hobo element invasion into D. melanogaster in the mid-1950s. In recently collected populations, H1 strains (low hobo activity and high repression potency) are predominant, whereas H þ strains (high hobo activity and high repression potency) are predominant in the Sakishima Islands, the most southwestern islands of the Japanese archipelago. H 0 strains (high hobo activity and low repression potency) were first found in limited island populations. Japanese populations have not only full-size hobo elements and 1.5 kb Th elements but also characteristic deletion derivatives (1.6 and 1.8 kb XhoI fragments) that we have named Jh elements. These results are consistent with transgenic experiments with complete hobo elements, in which populations evolved to H þ or H1 via H 0 , and in which 1.8 kb fragments appeared. We conclude that hobo elements invaded the central region of Japan, spread to the far islands, and that the invasion is currently at an intermediate, nonequilibrium stage.

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Mobilization of hobo elements residing within the decapentaplegic gene complex: Suggestion of a new hybrid dysgenesis system in Drosophila melanogaster

Cited by 184 publications

References 25 publications

A deletion-generator compound element allows deletion saturation analysis for genomewide phenotypic annotation

A deletion-generator compound element allows deletion saturation analysis for genomewide phenotypic annotation

Intrachromosomal excision of a hybrid Ds element induces large genomic deletions in Arabidopsis

Patterns of hobo elements and their effects in natural populations of Drosophila melanogaster in Japan

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