Mobilization and exocytosis of specific (secondary) granules by human neutrophils during adherence to nylon wood in filtration leukapheresis (FL)

Wright, DG; Kauffmann, JC; Gk, Terpstra; Graw, RG; Deisseroth, AB; Gallin, J I

doi:10.1182/blood.v52.4.770.bloodjournal524770

Blood

1978

DOI: 10.1182/blood.v52.4.770.bloodjournal524770

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Mobilization and exocytosis of specific (secondary) granules by human neutrophils during adherence to nylon wood in filtration leukapheresis (FL)

DG Wright¹,

JC Kauffmann²,

Terpstra Gk³

et al.

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1982

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“…Gallin and his coworkers (5,29,30) have pointed out that neutrophils release a small but significant portion of their content of specific but not azurophil granules whenever they adhere to a surface or move toward an inflammatory stimulus, through a filter in vitro or through tissue in vivo. These maneuvers have in common the requirement for a shape change.…”

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Degranulation, membrane addition, and shape change during chemotactic factor-induced aggregation of human neutrophils.

Hoffstein¹,

Friedman²,

Weissmann³

1982

The Journal of Cell Biology

125

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Neutrophils stimulated by the chemotactic factor formyl-methionyl-leucyl-phenylalanine (FMLP) undergo a transient change in surface properties that permits the cells to adhere more readily to surfaces and to each other. This transient change can be monitored by light scattering as stimulated neutrophils form aggregates while stirred in a platelet aggregometer. Maximum change in light scattering occurs within 1 min and correlates with an increase in the percentage of cells that are in aggregates of four or more cells and a decrease in the percentage of single cells. With time (3-5 min), small aggregates disappear and single cells reappear. The transient change in adhesiveness is accompanied by a persistent change in cell shape; the cells become polarized and protrude ruffles from one sector of the cell surface. During aggregation the cells adhere to one another with smooth sides together and ruffles pointed outward. During disaggregation the cells dissociate laterally with the simultaneous internalization of membrane in the region opposite the ruffles. Particle bound to the surface by charge (thorotrast, cationized ferritin) are concentrated and internalized in this region. The change in cell shape from round to ruffled occurs within seconds, suggesting that membrane is added to the cell surface from an intracellular store. We therefore quantified surface membrane by electron microscopy morphometry and measured a 25% increase within 10 s of adding FMLP. The source of new membrane appeared to be the specific granule membrane since the kinetics of granule discharge (between 30% and 50% of all release occurs in the first 10 s) correlate with the appearance of new membrane. Furthermore, the amount of membrane that appears at the cell surface at 10 s correlates with that lost from intracellular granules in that time. Chemotaxin-induced aggregation thus begins with granule discharge and membrane addition followed by protrusion of ruffles. Adherence is maximal at 60 s and the gradual loss of adhesiveness that follows is associated with uropod formation and enhanced endocytic activity.As has been well documented, neutrophils, after appropriate stimulation, rapidly remodel their surfaces. They undergo shape changes (24, 3 l), protrude ruffles, flatten, and spread on substrates. These activities would seem to require more surface area than is available in a rounded, resting neutrophfl, since the surface to volume ratio of a flat object with ruffles is greater than that of a round object with few surface productions. These changes in cell shape are so rapid that it appears likely that the new membrane which forms ruffles must arise from a preformed intraceUular store.One possible source of preformed membrane is the large population of secretory granules that these cells contain. Granules in neutrophils are of two basic types, azurophilic and specific. Azurophilic granules are homologous with lysosomes in most other cells but contain, in addition to the hydrolytic enzymes active at acid pH, neutral proteases and the...

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mentioning

confidence: 99%

Degranulation, membrane addition, and shape change during chemotactic factor-induced aggregation of human neutrophils.

Hoffstein¹,

Friedman²,

Weissmann³

1982

The Journal of Cell Biology

125

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Mobilization and exocytosis of specific (secondary) granules by human neutrophils during adherence to nylon wood in filtration leukapheresis (FL)

Cited by 1 publication

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Degranulation, membrane addition, and shape change during chemotactic factor-induced aggregation of human neutrophils.

Degranulation, membrane addition, and shape change during chemotactic factor-induced aggregation of human neutrophils.

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