Mobility of pyrene on chemically modified silica surfaces

Staahlberg, Jan.; Almgren, Mats; Alsins, Jan

doi:10.1021/ac00173a011

Cited by 41 publications

(25 citation statements)

References 36 publications

(22 reference statements)

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“…The use of fluorescence to characterize silica surfaces is well-documented, especially for silica-based materials in chromatography. Specifically, fluorescence-based experiments on covalently attached [42][43][44][45][46][47][48][49][50][51][52][53][54] or physiosorbed probes [55][56][57][58][59][60][61][62][63] have been carried out to gain a better understanding of the accessibility, distribution, mobility, and orientation of surface-bound molecules (e.g., catalysts, ligands, reagents, and recognition elements).…”

Section: Introductionmentioning

confidence: 99%

Comparison of Dansylated Aminopropyl Controlled Pore Glass Solvated by Molecular and Ionic Liquids

et al. 2006

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We compare how (i) four ionic liquids (ILs) (1-butyl-3-methylimidazolium tetrafluoroborate ([C4mim][BF4]), 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide ([C4mim][Tf2N]), 1-butyl-1-methylpyrrolidinium bis(trifluoromethylsulfonyl)imide ([C4mpy][Tf2N]), and trihexyltetradecylphosphonium bis(trifluoromethylsulfonyl)imide ([P(C6)3C14][Tf2N])) and (ii) two conventional molecular liquids (methanol and 1-octanol) solvate/wet luminescent organic moieties that are covalently attached to the surface of silica controlled pore glass (CPG). A series of aminopropyl CPG particles that have been covalently tagged with the solvatochromic fluorescent probe group dansyl were used in this study. The results demonstrate that ILs solvate/wet the silica surface differently in comparison to molecular liquids (MLs). Specifically, when comparing ILs and MLs that appear to solvate the free probe, dansylpropylsulfonamide (DPSA), equally in solution, we find that ILs do not solvate/wet the silica surfaces as well as the corresponding MLs. The cation component in these ILs is the significant factor in how the ILs solvate/wet silica surfaces. Solvation/wetting of surface-bound species at a silica surface depends on the cation size. Chlorosilane end-capping of the surface silanol and amine residues attenuates the cation's affects.

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Section: Introductionmentioning

confidence: 99%

Comparison of Dansylated Aminopropyl Controlled Pore Glass Solvated by Molecular and Ionic Liquids

et al. 2006

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“…The rate of excimer formation has been used to investigate the lateral diffusion of pyrene. 9,10 Fluorescence recovery after photobleaching is a more general method that has been used to m easure lateral transport of hydrophobic 11,12 and amphiphilic [13][14][15] uorophors at chromatographic interfaces. The limitations of this technique are that photobleaching can perturb the sample, either by heat or by introduction of photolysis products, and that not all uorophors photobleach readily.…”

Section: Introductionmentioning

confidence: 99%

Single-Molecule Spectroscopy and Fluorescence Correlation Spectroscopy of the Lateral Transport of the T3 Promoter Primer at a Chemical Interface

Wirth

Swinton

2001

Appl Spectrosc

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Fluorescence correlation spectroscopy reveals that an oligonucleotide, the T3 promoter primer, undergoes only lateral diffusion when adsorbed to the interface of water and silica chemically modified with a hydrocarbon. The autocorrelation decay fits well to the model of simple diffusion, reporting a diffusion coefficient of 1.8 × 10−6 cm2/s. Single-molecule resolution of bursts for the T3 promoter primer reveals that rare, strong adsorption punctuates the lateral diffusion. Removal of the strong adsorption events from the data set, followed by autocorrelation, shows the actual diffusion coefficient to be 2.8 × 10−6 cm2/s, which is comparable to other oligonucleotides of the same size at the same interface. The single-molecule measurements show that average duration of strong adsorption is 0.2 s, and the average fraction of strongly adsorbed molecules is 10% of the molecules at the interface. While single-molecule spectroscopy reveals a process not evident in fluorescence correlation spectroscopy, the precision of the parameters describing strong adsorption is limited by the statistics of small numbers. Fluorescence correlation spectroscopy is suited to observing a much larger number of events, which is needed for high precision. The two methods are complementary: single-molecule spectroscopy gives estimates of the chemical parameters needed for design of the fluorescence correlation spectroscopy, achieving precise measurements with an accurate interpretation.

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“…Separation effectiveness is largely determined by the distribution and conformation of the bonded phase. A number of spectroscopic methods have been employed to determine the stationaryphase polarity (3,4) and viscosity (5), the association between alkyl chains (6), and the degree of chain extension (7). In particular, the sensitivity of fluorescence measurements to the microenvironment of a probe molecule has been shown to provide considerable information concerning the stationaryphase environment (3)(4)(5)(6)(7)(8)(9)(10)(11)(12).…”

Section: Introductionmentioning

confidence: 99%

“…A number of spectroscopic methods have been employed to determine the stationaryphase polarity (3,4) and viscosity (5), the association between alkyl chains (6), and the degree of chain extension (7). In particular, the sensitivity of fluorescence measurements to the microenvironment of a probe molecule has been shown to provide considerable information concerning the stationaryphase environment (3)(4)(5)(6)(7)(8)(9)(10)(11)(12). Microenvironment polarity and viscosity have been determined by the examination of spectral wavelength shifts and the excited-state lifetimes of the fluorescent solutes in the bulk solution (2)(3)(4)12).…”

Section: Introductionmentioning

confidence: 99%

Investigation of the microenvironment polarity of a chromatographic surface using total internal reflection fluorescence

Rangnekar¹,

Oldham²

1990

Anal. Chem.

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A planar chromatographic surface was studied for microenvironmental polarity by using total Internal reflection fluorescence (TIRF). Pyrene was adsorbed on two different chemically modified quartz plates. The surfaces of the modified quartz plates were covered with either a monomeric C18 or a polymeric C1B layer. TIRF data were obtained for the adsorbed pyrene In the presence of different polar solvents. As the polarity of the solvents Increased, an overall Increase In fluorescence Intensity was observed and a change In the relative vlbronlc band Intensities (III/I) of pyrene was noted. Also, variable-angle TIRF data were collected for the adsorbed pyrene In the presence of a 40% methanol/water mixture (v/v). The fluorescence contribution from the bulk fluorescence was found to decrease as the Incidence angle Increased, as was expected.

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Mobility of pyrene on chemically modified silica surfaces

Cited by 41 publications

References 36 publications

Comparison of Dansylated Aminopropyl Controlled Pore Glass Solvated by Molecular and Ionic Liquids

Comparison of Dansylated Aminopropyl Controlled Pore Glass Solvated by Molecular and Ionic Liquids

Single-Molecule Spectroscopy and Fluorescence Correlation Spectroscopy of the Lateral Transport of the T3 Promoter Primer at a Chemical Interface

Investigation of the microenvironment polarity of a chromatographic surface using total internal reflection fluorescence

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