Mobility of photosynthetic complexes in thylakoid membranes

Mullineaux, Conrad W.; Tobin, Mark J.; Jones, Gareth R.

doi:10.1038/37157

Cited by 215 publications

(221 citation statements)

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“…The most plausible cause of such an extreme decrease in the diffusion coefficient is a strong interaction with another, immobile component in the membrane (Zhang et al, 1993). FRAP studies have shown that PSII is completely immobile under normal conditions (Mullineaux et al, 1997;Sarcina et al, 2001). We have no direct information on the diffusion of PSI, but its diffusion is likely to be very slow in the crowded environment of a cyanobacterial thylakoid membrane (Mullineaux, 1999).…”

Section: Discussionmentioning

confidence: 86%

“…Previous FRAP studies have shown that the phycobilisomes are highly mobile complexes, diffusing rapidly on the thylakoid membrane (Mullineaux et al, 1997;Sarcina et al, 2001). Figure 6 shows the rapid diffusion of phycobilisomes under physiological con- ditions in vivo.…”

Section: Discussionmentioning

confidence: 99%

“…The laser power was then reduced by a factor of 8 to prevent further bleaching, and the cell was repeatedly imaged. Phycobilisome diffusion causes the bleached line to spread and fill in, and the phycobilisome diffusion coefficient can be calculated from the rate of recovery of fluorescence at the center of the bleach (Mullineaux et al, 1997;Mullineaux and Sarcina, 2002). Figure 6 shows a typical result for a cell in growth medium.…”

Section: Resultsmentioning

confidence: 99%

“…FRAP measurements show that the phycobilisomes are highly mobile, with diffusion coefficients typically around 4 3 10 210 cm 2 s 21 (Sarcina et al, 2001;Aspinwall et al, 2004). By contrast, PSII appears completely immobile (Mullineaux et al, 1997;Sarcina et al, 2001). This indicates that there is no stable association between phycobilisomes and PSII, and it is likely that the association of phycobilisomes with PSI is also transient.…”

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Phycobilisome Diffusion Is Required for Light-State Transitions in Cyanobacteria

2004

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Phycobilisomes are the major accessory light-harvesting complexes of cyanobacteria and red algae. Studies using fluorescence recovery after photobleaching on cyanobacteria in vivo have shown that the phycobilisomes are mobile complexes that rapidly diffuse on the thylakoid membrane surface. By contrast, the PSII core complexes are completely immobile. This indicates that the association of phycobilisomes with reaction centers must be transient and unstable. Here, we show that when cells of the cyanobacterium Synechococcus sp. PCC7942 are immersed in buffers of high osmotic strength, the diffusion coefficient for the phycobilisomes is greatly decreased. This suggests that the interaction between phycobilisomes and reaction centers becomes much less transient under these conditions. We discuss the possible reasons for this. State transitions are a rapid physiological adaptation mechanism that regulates the way in which absorbed light energy is distributed between PSI and PSII. Immersing cells in high osmotic strength buffers inhibits state transitions by locking cells into whichever state they were in prior to addition of the buffer. The effect on state transitions is induced at the same buffer concentrations as the effect on phycobilisome diffusion. This implies that phycobilisome diffusion is required for state transitions. The main physiological role for phycobilisome mobility may be to allow such flexibility in light harvesting.

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

Section: Resultsmentioning

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Phycobilisome Diffusion Is Required for Light-State Transitions in Cyanobacteria

2004

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“…Unlike LHC I in chloroplasts, phycobilisomes are mobile and shared by PS I and PS II. By shifting phycobilisomes from one photosystem to the other, cyanobacteria are believed to accomplish state transitions (Mullineaux et al 1997).…”

Section: Introductionmentioning

confidence: 99%

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Mangels

Kruip

Berry

et al. 2002

Photosynthesis Research

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Photosystem I from the unusual cyanobacterium Gloeobacter violaceus Mangels, D.; Kruip, J.; Berry, S.; Rögner, M.; Boekema, E.J.; Koenig, F. Take-down policy If you believe that this document breaches copyright please contact us providing details, and we will remove access to the work immediately and investigate your claim.Downloaded from the University of Groningen/UMCG research database (Pure): http://www.rug.nl/research/portal. For technical reasons the number of authors shown on this cover page is limited to 10 maximum. Key words: antenna system, electron microscopy, fluorescence, light harvesting, photosynthesis, plasma membrane AbstractPhotosystem I (PS I) from the primitive cyanobacterium Gloeobacter violaceus has been purified and characterised. Despite the fact that the isolated complexes have the same subunit composition as complexes from other cyanobacteria, the amplitude of flash-induced absorption difference spectra indicates a much bigger antenna size with about 150 chlorophylls per P700 as opposed to the usual 90. Image analysis of the PS I preparation from Gloeobacter reveals that the PS I particles exist both in a trimeric and in a monomeric form and that their size and shape closely resembles other cyanobacterial PS I particles. However, the complexes exhibit a higher molecular weight as could be shown by gel filtration. The preparation contains novel polypeptides not related to known Photosystem I subunits. The N-terminal sequence of one of those polypeptides has been determined and reveals no homology to known or hypothetical proteins. Immunoblotting shows a cross-reaction of three of the polypeptide bands with an antibody raised against the major LHC from the diatom Cyclotella cryptica. Electron microscopy reveals a novel T-shaped complex which has never been observed in any other cyanobacterial PS I preparation. 77 K spectra of purified PS I show an extreme blue-shift of the fluorescence emission, indicating an unusual organisation of the PS I antenna system in Gloeobacter.Abbreviations: PS -photosystem; Chl -chlorophyll; DM -dodecyl-β-D-maltoside; ECL -enhanced chemiluminescence; HPLC -high performance liquid chromatography; IEC -ion exchange chromatography; GF -gel filtration; LHC I -light harvesting complex associated with Photosystem I; P700 -primary electron donor of photosystem I; SDS -sodium dodecyl sulfate; SQDG -sulfoquinovosyl diacylglycerol; PAGE -polyacrylamide gel electrophoresis; F760 (F730) -fluorescence band with maximal emission at 760 nm (730 nm)

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Redox and Photo Functions of Metal Complex Oligomer and Polymer Wires on the Electrode

Miyachi

Nishihara

2010

Macromolecules Containing Metal and Metal‐Like Elements

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Mobility of photosynthetic complexes in thylakoid membranes

Cited by 215 publications

References 21 publications

Phycobilisome Diffusion Is Required for Light-State Transitions in Cyanobacteria

Phycobilisome Diffusion Is Required for Light-State Transitions in Cyanobacteria

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Redox and Photo Functions of Metal Complex Oligomer and Polymer Wires on the Electrode

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