Abstract:Plant phenotyping and production management are emerging fields to facilitate Genetics, Environment, & Management (GEM) research and provide production guidance. Precision indoor farming systems (PIFS), vertical farms with artificial light (aka plant factories) in particular, have long been suitable production scenes due to the advantages of efficient land utilization and year-round cultivation. In this study, a mobile robotics platform (MRP) within a commercial plant factory has been developed to dyna… Show more
“…For example, the metabolite and lipid composition of plants can be modulated by selecting specific light wavelengths for illumination ( Rihan et al, 2022 ), and can thus help to reduce the concentration of potentially harmful metabolites like alkaloids (see Section 2.1.1 ). Furthermore, CEGS can be fully automated so that human intervention and ultimately the risk of contamination with human pathogens is minimized ( Wirz et al, 2012 ; Huebbers and Buyel, 2021 ; Ren et al, 2023 ). The high degree of automation/mechanization in such systems, and the close proximity of the corresponding devices and plants, increases the likelihood that the product will come into contact with auxiliary and operating materials such as lubricants.…”
Section: Potential Risks Arising From Bioprocess Designmentioning
Plant molecular farming (PMF) has been promoted since the 1990s as a rapid, cost-effective and (most of all) safe alternative to the cultivation of bacteria or animal cells for the production of biopharmaceutical proteins. Numerous plant species have been investigated for the production of a broad range of protein-based drug candidates. The inherent safety of these products is frequently highlighted as an advantage of PMF because plant viruses do not replicate in humans and vice versa. However, a more nuanced analysis of this principle is required when considering other pathogens because toxic compounds pose a risk even in the absence of replication. Similarly, it is necessary to assess the risks associated with the host system (e.g., the presence of toxic secondary metabolites) and the production approach (e.g., transient expression based on bacterial infiltration substantially increases the endotoxin load). This review considers the most relevant host systems in terms of their toxicity profile, including the presence of secondary metabolites, and the risks arising from the persistence of these substances after downstream processing and product purification. Similarly, we discuss a range of plant pathogens and disease vectors that can influence product safety, for example, due to the release of toxins. The ability of downstream unit operations to remove contaminants and process-related toxic impurities such as endotoxins is also addressed. This overview of plant-based production, focusing on product safety aspects, provides recommendations that will allow stakeholders to choose the most appropriate strategies for process development.
“…For example, the metabolite and lipid composition of plants can be modulated by selecting specific light wavelengths for illumination ( Rihan et al, 2022 ), and can thus help to reduce the concentration of potentially harmful metabolites like alkaloids (see Section 2.1.1 ). Furthermore, CEGS can be fully automated so that human intervention and ultimately the risk of contamination with human pathogens is minimized ( Wirz et al, 2012 ; Huebbers and Buyel, 2021 ; Ren et al, 2023 ). The high degree of automation/mechanization in such systems, and the close proximity of the corresponding devices and plants, increases the likelihood that the product will come into contact with auxiliary and operating materials such as lubricants.…”
Section: Potential Risks Arising From Bioprocess Designmentioning
Plant molecular farming (PMF) has been promoted since the 1990s as a rapid, cost-effective and (most of all) safe alternative to the cultivation of bacteria or animal cells for the production of biopharmaceutical proteins. Numerous plant species have been investigated for the production of a broad range of protein-based drug candidates. The inherent safety of these products is frequently highlighted as an advantage of PMF because plant viruses do not replicate in humans and vice versa. However, a more nuanced analysis of this principle is required when considering other pathogens because toxic compounds pose a risk even in the absence of replication. Similarly, it is necessary to assess the risks associated with the host system (e.g., the presence of toxic secondary metabolites) and the production approach (e.g., transient expression based on bacterial infiltration substantially increases the endotoxin load). This review considers the most relevant host systems in terms of their toxicity profile, including the presence of secondary metabolites, and the risks arising from the persistence of these substances after downstream processing and product purification. Similarly, we discuss a range of plant pathogens and disease vectors that can influence product safety, for example, due to the release of toxins. The ability of downstream unit operations to remove contaminants and process-related toxic impurities such as endotoxins is also addressed. This overview of plant-based production, focusing on product safety aspects, provides recommendations that will allow stakeholders to choose the most appropriate strategies for process development.
Background
Pollination strategies to supplement or replace insect pollinators are needed to produce marketable strawberry fruits in indoor vertical farms. To ensure the self-pollination of strawberry flowers, we investigated the anther dehiscence and pollen attachment under different vapor pressure deficit (VPD) conditions and external mechanical sine wave vibrations.
Results
The proportion of dehisced anthers was examined under VPDs of 2.06, 1.58, and 0.33 kPa, and the projected area of pollen clumps was assessed under VPDs of 2.06 and 0.33 kPa. After exposing flowers to a VPD of 0.33 kPa, vibrations with various frequency (Hz) and root mean square acceleration (m s−2) combinations were used to evaluate pollination effectiveness. The anthers underwent complete dehiscence at VPDs of 2.06, 1.58, and 0.33 kPa. The projected area of the pollen clump was larger at a VPD of 0.33 than that at 2.06 kPa. Pollen clump detachment was effective at 100 and 800 Hz with 40 m s−2, while pollen attachment to the stigma was most effective at 100 Hz.
Conclusions
These findings suggest that environmental control strategies and specific vibration characteristics can successfully aid strawberry flower pollination. This study provides fundamental insights into mechanical vibration pollination systems for fruit production in indoor vertical farming.
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