Mobile phone radiofrequency exposure has no effect on DNA double strand breaks (DSB) in human lymphocytes

Danese, Elisa; Lippi, Giuseppe; Buonocore, Ruggero; Benati, Marco; Bovo, Chiara; Bonaguri, Chiara; Salvagno, Gian Luca; Brocco, Giorgio; Roggenbuck, Dirk; Montagnana, Martina

doi:10.21037/atm.2017.04.35

Cited by 16 publications

(11 citation statements)

References 36 publications

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“…However, the effects of radiofrequency on the blood cell count have been addressed in the past. It has been reported that the exposure of humans to commercial mobile phones for 30 minutes has no influences on lymphocyte count; our data showed the same result with jammer radiation as well [15]. No difference in RBC count has been reported in human exposed to EMF [16] which is in contrast with the result obtained by jammer exposure.…”

Section: Discussionsupporting

confidence: 84%

Electromagnetic Fields of Mobile Phone Jammer Exposure on Blood Factors in Rats

et al. 2018

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Background: The increasing demand for using mobile phones has led to increasing mobile phone jammers as well. On the other hand, reports show that exposure to electromagnetic field causes an increase in the incidence of diseases such as leukemia, cancer, depression and failure in pregnancy outcomes; therefore, the aim of this study is to investigate the effects of exposure to electromagnetic fields of mobile phone jammers on blood factors.Materials and Methods: Thirty male Wistar immature and thirty mature rats were selected randomly and each one was divided into three groups of ten. The control group did not receive any radiation; the sham group was exposed to a switched-off jammer device and the experimental group was exposed to electromagnetic fields (EMF) radiated by Mobile Phone Jammer daily eight hours for five days a week during forty days. Blood sample was taken from heart and blood factors including PLT, MCHC and RDWCV were measured. The data were analyzed by ANOVA which was followed by Duncan’s test.Results: The data from mature rats revealed that jammer usage led to a significant difference in blood factors including RBC, platelet, hemoglobin, hematocrit, MCV and RDWCV (P≤0.05); however, the number of lymphocytes, WBC and MCVH in the blood was the same in all groups. In immature rats, the exposure to jammer did not change RBC, lymphocyte and WBC count, hemoglobin and hematocrit; while, the platelet count along with MCHC, MVC and RDWCV changed by jammer radiation.Conclusion: The results exhibited that mobile phone jammer caused frequent changes in blood cell factors.

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Section: Discussionsupporting

confidence: 84%

Electromagnetic Fields of Mobile Phone Jammer Exposure on Blood Factors in Rats

et al. 2018

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“…DNA repair foci are formed by proteins such as γH2AX and 53BP1, which take part in DNA damage response and DSB repair 29,30 . Analysis of DSB after MW exposure revealed different data ranging from increased level of DSB 31,32 , no effect 21,24,33 up to inhibition of DSB repair 34–36 . At the same time, number of studies described increased level of DSB and single strand breaks (SSB) in rat and mouse brain cells using comet assay 37–40 .…”

Section: Introductionmentioning

confidence: 99%

Microwaves from mobile phone induce reactive oxygen species but not DNA damage, preleukemic fusion genes and apoptosis in hematopoietic stem/progenitor cells

Durdik

Košík

Markovà

et al. 2019

Sci Rep

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Exposure to electromagnetic fields (EMF) has been associated with the increased risk of childhood leukemia, which arises from mutations induced within hematopoietic stem cells often through preleukemic fusion genes (PFG). In this study we investigated whether exposure to microwaves (MW) emitted by mobile phones could induce various biochemical markers of cellular damage including reactive oxygen species (ROS), DNA single and double strand breaks, PFG, and apoptosis in umbilical cord blood (UCB) cells including CD34+ hematopoietic stem/progenitor cells. UCB cells were exposed to MW pulsed signals from GSM900/UMTS test-mobile phone and ROS, apoptosis, DNA damage, and PFG were analyzed using flow cytometry, automated fluorescent microscopy, imaging flow cytometry, comet assay, and RT-qPCR. In general, no persisting difference in DNA damage, PFG and apoptosis between exposed and sham-exposed samples was detected. However, we found increased ROS level after 1 h of UMTS exposure that was not evident 3 h post-exposure. We also found that the level of ROS rise with the higher degree of cellular differentiation. Our data show that UCB cells exposed to pulsed MW developed transient increase in ROS that did not result in sustained DNA damage and apoptosis.

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“…Two laboratory studies were found that employed real-life MT EMF exposure of human lymphocytes by commercially available mobile phones: In the older one (Ji et al 2004), volunteers were exposed in vivo by talking on their GSM (2G) mobile phones for 4 h. After the exposure, DNA damage in their blood samples was significantly increased compared to their blood samples before the exposure. In the newer study (Danese et al 2017) blood samples were exposed in vitro for 30 min to a GSM (2G) real signal emitted by an activated mobile phone and no significant effect on DNA double strand breaks was reported. While the authors of this study used a 3G mobile phone for the exposures, they did not test the 3G signal but the much older (and less bioactive) 2G signal.…”

Section: Introductionmentioning

confidence: 98%

Comparing chromosome damage induced by mobile telephony radiation and a high caffeine dose: Effect of combination and exposure duration

Panagopoulos

2020

gpb

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I recently reported induction of chromatid-type aberrations in human peripheral blood lymphocytes after a single 15 min exposure to Universal Mobile Telecommunications System (UMTS) Mobile Telephony (MT) Electromagnetic Field (EMF) from a mobile phone. Lymphocytes from six healthy subjects were stimulated for mitosis, and exposed during the G 2 /M phase at 1 cm distance from the handset during an active phone call in "talk" mode. The same type of cells from the same subjects treated with a high caffeine dose (~ 290 times above the permissible single dose for an adult human) exhibited the same type of aberrations in a little smaller but comparable degree. The combination of this caffeine dose and the 15 min MT EMF exposure increased dramatically the number of aberrations in all subjects. The combined effect increased almost linearly with increasing duration of exposure to the MT EMF. Thus, MT EMF exposure ~ 136 times below the official limit (ICNIRP 2020) exerts a genotoxic action even greater than that of a caffeine dose ~ 290 times above the corresponding limit. Therefore, with a reasonable approximation, the limit for MT EMFs should be lowered by at least ~ 4×10 4 times (136×290) for short-term exposures, and ~ 4×10 6 times for long-term exposures.

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Mobile phone radiofrequency exposure has no effect on DNA double strand breaks (DSB) in human lymphocytes

Cited by 16 publications

References 36 publications

Electromagnetic Fields of Mobile Phone Jammer Exposure on Blood Factors in Rats

Electromagnetic Fields of Mobile Phone Jammer Exposure on Blood Factors in Rats

Microwaves from mobile phone induce reactive oxygen species but not DNA damage, preleukemic fusion genes and apoptosis in hematopoietic stem/progenitor cells

Comparing chromosome damage induced by mobile telephony radiation and a high caffeine dose: Effect of combination and exposure duration

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