Mobile foci of Sp100 do not contain PML: PML bodies are immobile but PML and Sp100 proteins are not

Wiesmeijer, Karien C.

doi:10.1016/s1047-8477(02)00529-4

Cited by 57 publications

(45 citation statements)

References 41 publications

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“…Mobility of fission products of PML nuclear bodies is consistent with attachments to chromatin PML nuclear bodies are relatively immobile within the nucleus during extended periods of interphase (supplementary material Movie 4) (Eskiw et al, 2004;Eskiw et al, 2003;Wiesmeijer et al, 2002). We characterised the mobility of PML-containing structures in S phase in U-2 OS cells expressing GFP-PML IV (Fig.…”

Section: Pml Nuclear Body Number Increases Upon Entry Into S Phasesupporting

confidence: 58%

“…In normal cells, the PML protein is found in 5-30 sub-nuclear domains of approximately 0.3 to 1 m in diameter, which are known as PML nuclear bodies (Melnick and Licht, 1999). During extended periods in interphase, PML nuclear bodies are quite immobile and appear to maintain their relative positioning through extensive contacts with the surrounding chromatin (Eskiw et al, 2004;Eskiw et al, 2003;Wiesmeijer et al, 2002). By contrast, these bodies are highly dynamic following heat or heavy metal shock and in response to DNA damage.…”

Section: Introductionmentioning

confidence: 99%

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The number of PML nuclear bodies increases in early S phase by a fission mechanism

Dellaire

Ching

Dehghani

et al. 2006

Journal of Cell Science

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Promyelocytic leukemia (PML) nuclear bodies have been implicated in a variety of cellular processes including apoptosis, tumour suppression, anti-viral response, DNA repair and transcriptional regulation. PML nuclear bodies are both positionally and structurally stable over extended periods of interphase. As demonstrated in this study, the structural stability is lost as cells enter S phase, evidenced both by distortions in shape and by fission and fusion events. At the end of this period of structural instability, the number of PML nuclear bodies has increased by a factor of twofold. Association of the fission products with chromatin implies that the PML nuclear bodies respond to changes in chromatin organisation or topology, and thus could play a role in monitoring genome integrity during DNA synthesis or in the continued maintenance of functional chromosomal domains prior to mitosis.

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Section: Pml Nuclear Body Number Increases Upon Entry Into S Phasesupporting

confidence: 58%

Section: Introductionmentioning

confidence: 99%

The number of PML nuclear bodies increases in early S phase by a fission mechanism

Dellaire

Ching

Dehghani

et al. 2006

Journal of Cell Science

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“…Previous analyses on component exchange at PML NBs had used only the isoform PML IV in FRAP experiments (Boisvert et al, 2001;Wiesmeijer et al, 2002;Everett et al, 2005). In the present study we have analyzed all six nuclear PML isoforms by FRAP and FCS.…”

Section: Pml Traffic At Nbsmentioning

confidence: 97%

Dynamics of component exchange at PML nuclear bodies

Weidtkamp‐Peters¹,

Lenser

Negorev

et al. 2008

Journal of Cell Science

183

167

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PML nuclear bodies (NBs) are involved in the regulation of key nuclear pathways but their biochemical function in nuclear metabolism is unknown. In this study PML NB assembly dynamics were assessed by live cell imaging and mathematic modeling of its major component parts. We show that all six nuclear PML isoforms exhibit individual exchange rates at NBs and identify PML V as a scaffold subunit. SP100 exchanges at least five times faster at NBs than PML proteins. Turnover dynamics of PML and SP100 at NBs is modulated by SUMOylation. Exchange is not temperature-dependent but depletion of cellular ATP levels induces protein immobilization at NBs. The PML-RARα oncogene exhibits a strong NB retention effect on wild-type PML proteins. HIPK2 requires an active kinase for PML NB targeting and elevated levels of PML IV increase its residence time. DAXX and BLM turn over rapidly and completely at PML NBs within seconds. These findings provide a kinetics model for factor exchange at PML NBs and highlight potential mechanisms to regulate intranuclear trafficking of specific factors at these domains.

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“…2 Under normal growth conditions, almost every mammalian cell usually harbours between 10 and 30 doughnut-like-shaped PMLNBs with a diameter ranging between 0.2 and 1 mm. [3][4][5][6][7] Besides a number of viral proteins, to date more than 40 different cellular proteins have been found in association with PML-NBs ( Figure 1). Although the molecular function of PMLNBs is currently not clear, there is accumulating evidence that PML-NBs are regulatory domains involved in various biological processes, including protein degradation, 5 transcriptional regulation, 8,9 cell growth, 10,11 antiviral response, 12,13 cellular senescence, [14][15][16][17] tumour suppression, 18 DNA repair 19,20 and apoptosis.…”

Section: Introductionmentioning

confidence: 99%

Body language: the function of PML nuclear bodies in apoptosis regulation

2003

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Promyelocytic leukaemia (PML) nuclear bodies (NBs) are macromolecular nuclear domains present in virtually every mammalian cell. PML nuclear bodies (PML-NBs) were functionally linked to various fundamental cellular processes, including transcriptional control, tumour suppression and apoptosis regulation. Supporting the important function of PML and its associated NBs in apoptosis regulation, several apoptotic regulators localise to PML-NBs, and cells from PMLdeficient mice show severe apoptotic defects, including induction of genotoxic stress and death receptor CD95 (Fas/ APO-1) activation. Based on the current literature, we hypothesise that PML-NBs regulate apoptosis through different molecular mechanisms, on the one hand by acting as macromolecular scaffolds for recruitment and post-translational modification of the apoptotic key regulator p53, and on the other by regulating the subcellular bioavailability and quality of some apoptotic signal transducers.

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Mobile foci of Sp100 do not contain PML: PML bodies are immobile but PML and Sp100 proteins are not

Cited by 57 publications

References 41 publications

The number of PML nuclear bodies increases in early S phase by a fission mechanism

The number of PML nuclear bodies increases in early S phase by a fission mechanism

Dynamics of component exchange at PML nuclear bodies

Body language: the function of PML nuclear bodies in apoptosis regulation

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