Mobile dispersed genetic element MDGI of Drosophila melanogaster: transcription pattern

Ilyin, Yu. V.; Chmeliauskate, V.G.; Kulguskin, V.V.; Georgiev, G.P.

doi:10.1093/nar/8.22.5347

Cited by 15 publications

(4 citation statements)

References 11 publications

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“…PCR products carrying T7 promoter or plasmids containing the cloned fragments of retrotransposons were used as transcription templates. mdg1 : 0.35 kb EcoRV–NsiI fragment from plasmid Dm688 (55) was subcloned into pBS SK − and used to obtain sense or antisense riboprobe. 1731 : PCR-amplified fragment using primers (5′-ACGAATTCAGCGAACTGTCGTCG-3′) and (5′-ATGGATCCATGTGACTGGTAGC-3′) corresponding to 1138–1271 nt (GenBank accession no.…”

Section: Methodsmentioning

confidence: 99%

Argonaute protein PIWI controls mobilization of retrotransposons in the Drosophila male germline

Kalmykova¹

2005

Nucleic Acids Research

198

135

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Proteins of the Argonaute family have been identified as key components of RNA interference (RNAi) pathway. RNAi-related mechanisms are implicated in the regulation of gene expression and repression of transposable elements in eukaryotes. The piwi gene encoding protein of the Drosophila Argonaute family was shown to be required for the germ stem cells maintenance. Here, we show that piwi is involved in silencing of LTR retrotransposons in testes. piwi mutations led to derepression of endogenous retrotransposon copia as well as to upregulation of the reporter gene driven by copia LTR. piwi mutation causes accumulation of retrotransposon mdg1 transcripts at the apical tip of testes, including germinal proliferative center where PIWI protein was shown to be expressed. We applied inverse PCR approach to detect the newly arisen insertions of the mdg1 retrotransposon in the progeny of individual piwi mutant males. Owing to piwi mutation a high rate of mdg1 transpositions was revealed. Thus, piwi is involved in the silencing of retrotransposons in the precursors of male gametes. Our results provide the first evidence that protein of the Argonaute family prevents retrotranspositions. It is supposed that the disturbance of RNA silencing system in germinal cells might cause transposition burst.

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Section: Methodsmentioning

confidence: 99%

Argonaute protein PIWI controls mobilization of retrotransposons in the Drosophila male germline

Kalmykova¹

2005

Nucleic Acids Research

198

135

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“…Only the full-length transcripts were detected after transfection of Drosophila cultured cells with the plasmid containing the fulllength mdgl copy (pF), (fig.2B, lane 3). The pattern of transcripts was the same for D.melanogaster cell lines Schneider 2 (fig.4, lane 1) and 67J25D (11). Analysis of the mdgl transcription pattern in flies and larvae of some strains revealed 1.5 kb transcripts in larvae of Domodedovo strain (fig.4, lane 2).…”

Section: Analysis Of Mdgl Endogenous Transcriptsmentioning

confidence: 71%

“…The significance of the cryptic premature polyadenylation is not completely clear, but it possibly can be one of the factors restricting the full-length gene transcription. mdgl has a strong promoter and it is transcribed very actively (11). So, the restriction of transcription may regulate transposition frequency of the element.…”

Section: Discussionmentioning

confidence: 99%

Leader region of mdg1Drosophilaretrotransposon RNA contains 3′-end processing sites

1991

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Transient expression of the mdg1 deletion mutants revealed sites of 3'-end processing in the leader region of the transcribed RNA. The efficiency of the processing is regulated in different types of cells. The sequences within the mdg1 body and the 3'-LTR are involved in its regulation. We have also shown, that one of the small open reading frames in the mdg1 leader region in principle might be translated.

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“…Then RNA molecules are transported into the cytoplasm, and some (no more than 10-20%) form polysomes [7,8]. Their translation yields proteins that bind with other retrotransposon transcripts to form VLPs.…”

Section: Intracellular Replication Cyclementioning

confidence: 99%

Diversity of LTR retrotransposons and their role in genome reorganization

Syomin

Ilyin

2005

Russ J Genet

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Current views of retrotransposons possessing long terminal repeats (LTRs) are described. The existing classification and element types isolated by genome organization are considered. Experimental data are summarized to demonstrate that the replicative cycle of a retrotransposon is not restricted to a single cell and that LTR retrotransposons are transferred between somatic cells with a rate comparable with the element transposition rate within the genome of one cell. The major mechanisms mediating the role of LTR retrotransposons in reorganization of the genome are considered with regard to the strategies of their horizontal and vertical transfer.

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Mobile dispersed genetic element MDGI of Drosophila melanogaster: transcription pattern

Cited by 15 publications

References 11 publications

Argonaute protein PIWI controls mobilization of retrotransposons in the Drosophila male germline

Argonaute protein PIWI controls mobilization of retrotransposons in the Drosophila male germline

Leader region of mdg1Drosophilaretrotransposon RNA contains 3′-end processing sites

Diversity of LTR retrotransposons and their role in genome reorganization

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