Mobile dispersed genetic element MDGI of Drosophila melanogaster: structural organization

Ilyin, Yu. V.; Chmeliauskate, V.G.; Ananiev, E. V.; Lyubomirskaya, N. V.; Kulguskin, V.V.; Bayev, A.A.; Georgiev, G.P.

doi:10.1093/nar/8.22.5333

Cited by 24 publications

(1 citation statement)

References 16 publications

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“…Although originally isolated as a class of moderately repetitious DNA encoding abundantly expressed RNA (1), copia and several analogous sequence fami l ies have generated wide interest owing to thei r abi li ty to transpose to a large number of chromosomal sites in the Drosophila genome (2)(3)(4). Many additional moderately repeated sequence families have been described in Drosophila which share at least some of the properties of the copia sequence family (4)(5)(6)(7)(8) and together these sequences probably constitute about 5% of the Drosophila genome.…”

Section: Introductionmentioning

confidence: 99%

The 5′ termini of RNAs encoded by the transposable element copia

et al. 1981

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The 5' termini of copia-specific RNAs in Drosophila melanogaster tissue culture cells were determined by S1 nuclease mapping and cap analysis. Both major copia RNAs share an identical set of heterogeneous 5' ends. Three major cap 1 structures M7GpppCmpUp, M7GpppCmpCp and M7GpppGmpUp together with several other minor caps were found. Almost all the 5' termini, as judged by S-1 nuclease mapping, were located either in a pyrimidine-rich part of the terminal direct repeat or apparently outside of the copia element, suggesting that a proportion of copia transcripts derive from promoters external to the genetic element.

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Section: Introductionmentioning

confidence: 99%

The 5′ termini of RNAs encoded by the transposable element copia

et al. 1981

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References

Galun

2003

Transposable Elements

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Chromosomal locations of two DNA segments that flank ribosomal insertion-like sequences in Drosophila: Flanking sequences are mobile elements

Pardue

Dawid

1981

Chromosoma

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We report the chromosomal locations of two repetitive DNA sequences that flank ribosomal insertion-like sequences in Drosophila melanogaster. The chromocentric region of D. melanogaster contains many copies of sequences that are homologous to type 1 ribosomal insertions. These insertion-like elements are interspersed with other DNA segments that we call flanking sequences. Two distinct flanking sequences derived from the same cloned DNA molecule pDmI 101, the HindIII fragments 101E and 101F, were studied. Whole genome Southern blots with DNA from the D. melanogaster stocks Oregon R (P2), gt-1, and gt-X11 showed complex restriction patterns that differed substantially between the three stocks. This and other data show that flanking sequences are members of diverged repetitive sequence families. In situ hybridization to salivary gland chromosomes of gt-1 and gt-X11 showed that both sequences are homologous to the chromocenter and to about 5 to 8 (101E) or 25 to 30(101F) euchromatic sites in each stock. Most, if not all, of these sites differed in gt-1 and gt-X11. Both 101E and 101F are homologous to he chromocenter and very few euchromatic bands in D. simulans, but 101F is homologous to numerous bands in D. mauritiana. We conclude that the flanking sequences represented by 101E and 101F are mobile elements within the genome of Drosophila. These two sequences differ in several structural features from mobile DNA elements previously described in this organism.

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Mobile dispersed genetic element MDGI of Drosophila melanogaster: structural organization

Cited by 24 publications

References 16 publications

The 5′ termini of RNAs encoded by the transposable element copia

The 5′ termini of RNAs encoded by the transposable element copia

References

Chromosomal locations of two DNA segments that flank ribosomal insertion-like sequences in Drosophila: Flanking sequences are mobile elements

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