Mobile Device for Disease Diagnosis and Data Tracking in Resource-Limited Settings

Chin, Curtis D.; Cheung, Yuk Kee; Laksanasopin, Tassaneewan; Modena, Mario M.; Chin, Sau Yin; Sridhara, Archana A.; Steinmiller, David; Linder, Vincent; Mushingantahe, Jules; Umviligihozo, Gisele; Karita, Etienne; Mwambarangwe, Lambert; Braunstein, Sarah L.; Wijgert, Janneke van de; Sahabo, Ruben; Justman, Jessica; El‐Sadr, Wafaa; Sia, Samuel K.

doi:10.1373/clinchem.2012.199596

Cited by 72 publications

(54 citation statements)

References 34 publications

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“…Note, the LOD of this system has been established as 1000 copies mL −1 . In other research, Laksanasopin et al created a low power consumption smartphone attachment capable of triplexed ELISA assays specific to HIV and syphilis [25,105]. Further, cellphone-based optical devices have also been used for early diagnosis of human eye cataracts [106] without expensive equipment or highly trained medical staff.…”

Section: Cellphone-based Diagnostics Toolsmentioning

confidence: 99%

Cellphone-based diagnostic devices

Huang¹,

Cheng²

2017

Point-of-Care Diagnostics - New Progresses and Perspectives

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Section: Cellphone-based Diagnostics Toolsmentioning

confidence: 99%

Cellphone-based diagnostic devices

Huang¹,

Cheng²

2017

Point-of-Care Diagnostics - New Progresses and Perspectives

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“…For cases in which the calculated sensitivity or specificity was equal to 1, a P value of 0.999 was used to calculate 95% CIs, as recommended by WHO (37 ) and others (17,18 ).…”

Section: Evaluation Of Diagnostic Accuracymentioning

confidence: 99%

“…This model requires portable assays, and the microfluidic (or "lab on a chip") community has attempted to address this challenge primarily through systems that rely on fluid flow through enclosed microchannels (10 -12 ) or lateral flow through a paper-based absorptive matrix (13)(14)(15). Microchannel-based systems have excellent analytical sensitivity (16 ) and proven diagnostic performance in resource-limited settings (17)(18)(19), but often require extensive ancillary equipment to operate (e.g., pumps, flow meters, and valves) and intricate device fabrication for integrated sample preparation (20 ). In contrast, paperbased systems can be simple and inexpensive to manufacture and use (21 ), but adequate analytical performance in such devices has been demonstrated for only a few analytes (15 ), and multistep liquid manipulation with uniform flow profiles requires careful material manipulation and device design (22)(23)(24).…”

mentioning

confidence: 99%

Digital Microfluidic Platform for the Detection of Rubella Infection and Immunity: A Proof of Concept

Lee

Choi

et al. 2015

Clinical Chemistry

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BACKGROUND:Whereas disease surveillance for infectious diseases such as rubella is important, it is critical to identify pregnant women at risk of passing rubella to their offspring, which can be fatal and can result in congenital rubella syndrome (CRS). The traditional centralized model for diagnosing rubella is cost-prohibitive in resource-limited settings, representing a major obstacle to the prevention of CRS. As a step toward decentralized diagnostic systems, we developed a proof-of-concept digital microfluidic (DMF) diagnostic platform that possesses the flexibility and performance of automated immunoassay platforms used in central facilities, but with a form factor the size of a shoebox.

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“…For reasons including convenience and privacy, reproductive health has already proven to be an important area for decentralizing blood testing. This practical and promising study of Nosrati et al complements other next-generation diagnostic tests for reproductive health that have been tested on clinical samples (4,5,7,8 ), as well as recently approved home tests such as the HIV test (9 ). More broadly, advances such as the test reported by Nosrati et al could dramatically expand the capabilities of consumer health testing devices, which are already prevalent for activity tracking and personalized genomics testing.…”

mentioning

confidence: 91%

Toward a Microfluidics-Based Home Male Fertility Test

2016

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Consumers are increasingly interested in knowing more about their own health. One of the most familiar directto-consumer diagnostics tests has been the home pregnancy test. In this issue of Clinical Chemistry, Nosrati et al.(1 ) report their clever and practical approach toward developing a new point-of-care diagnostic test for another reproductive health need-the assessment of male fertility.The authors developed the test using paper, a familiar substrate used in pregnancy tests and the subject of a flurry of recent improvements in microfluidics (2 ). The authors placed at the bottom one laminate layer, and on top, paper layers patterned with wax (3 ). With the paper test they aimed to quantify sperm concentration via a dye that changes color in the presence of an enzyme (diaphorase flavoprotein) that resides within metabolically active human sperm. In one spot on the paper, the sample reacts directly with the dried dye. In a second spot, sperm must swim vertically through a highly viscous buffer and a membrane filter to generate a colorimetric signal. Ten minutes later, a scanner reads the color intensities of the 2 spots, which are used to quantify live and motile sperm concentrations.The paper diagnostic test was assessed on 17 frozen human semen samples obtained from a hospital. Although the performance was promising (for example, it predicted correctly the 1 out of 6 samples having low sperm motility), it is increasingly recognized in the research field of microfluidics that there exists a yawning gap between an academic demonstration and clinical utility. Setting their test apart from many academic demonstrations, these investigators studied the performance of the test over different times of storage and at different humidity levels. Their device, when packaged with desiccant, demonstrated only a small degradation in color change over 16 weeks even in the presence of 22.8 g/m 3 humidity (equivalent to about the maximum humidity at room temperature). For point-of-care use, building a rugged and stable test is critical in translating a promising technology to the clinic.Not all clinical diagnostic tests that can be done in a point-of-care format should be done in a point-of-care format. One driver that justifies a consumer-facing diagnostic test is to provide information that would motivate patients to purchase and use the product. In line with the success of the home pregnancy test, the authors chose an application that would appeal to consumer use. In this case, assessment of male fertility is often initiated by the consumer in cases in which a couple has difficulty conceiving. Moreover, obtaining semen samples would be more comfortably performed at home and in private, which makes this application perfectly suited for home use. Although such considerations may appear more relevant at a later stage of commercialization, understanding them is important at an early research stage to develop a device with proper target specifications (sample type, simplicity of design, resources available) for the intended c...

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Mobile Device for Disease Diagnosis and Data Tracking in Resource-Limited Settings

Cited by 72 publications

References 34 publications

Cellphone-based diagnostic devices

Cellphone-based diagnostic devices

Digital Microfluidic Platform for the Detection of Rubella Infection and Immunity: A Proof of Concept

Toward a Microfluidics-Based Home Male Fertility Test

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