Mobile ARGONAUTE 1d binds 22-nt miRNAs to generate phasiRNAs important for low-temperature male fertility in rice

Si, Fuyan; Luo, H.; Yang, Chao; Gong, Jie; Yan, Bin; Liu, Chunyan; Song, Xianwei; Cao, Xiaofeng

doi:10.1007/s11427-022-2204-y

Cited by 16 publications

(8 citation statements)

References 56 publications

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“…Intriguingly, two independent groups recently reported that not only sRNA cargoes but also the AGO protein itself could traffic between adjacent cells or different cell types, as demonstrated for two AGO1 sub-clade members. Comparing with the positions of their mRNA transcripts and mature proteins revealed that AGO1b and AGO1d serve as mobile signals during rice reproductive development [94,95]. These new findings suggest that there may be distinct properties of AGO proteins in different plant tissues.…”

Section: Srna Traffickingmentioning

confidence: 80%

Beyond Loading: Functions of Plant ARGONAUTE Proteins

Liang,

Wang,

et al. 2023

IJMS

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ARGONAUTE (AGO) proteins are key components of the RNA-induced silencing complex (RISC) that mediates gene silencing in eukaryotes. Small-RNA (sRNA) cargoes are selectively loaded into different members of the AGO protein family and then target complementary sequences to in-duce transcriptional repression, mRNA cleavage, or translation inhibition. Previous reviews have mainly focused on the traditional roles of AGOs in specific biological processes or on the molecular mechanisms of sRNA sorting. In this review, we summarize the biological significance of canonical sRNA loading, including the balance among distinct sRNA pathways, cross-regulation of different RISC activities during plant development and defense, and, especially, the emerging roles of AGOs in sRNA movement. We also discuss recent advances in novel non-canonical functions of plant AGOs. Perspectives for future functional studies of this evolutionarily conserved eukaryotic protein family will facilitate a more comprehensive understanding of the multi-faceted AGO proteins.

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Section: Srna Traffickingmentioning

confidence: 80%

Beyond Loading: Functions of Plant ARGONAUTE Proteins

Liang,

Wang,

et al. 2023

IJMS

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“…Recent reproductive studies, revealing the cell-to-cell transportation of 24-nt phasiRNAs and the regulation of pollen development by somatic transcription factors, also imply non-cell-autonomous regulation in anther development 18 , 36 . Differential localization between AGO1d mRNA and AGO1d protein, which functions in temperature-dependent anther development, has been reported 37 , 38 . We therefore propose that AGO1b/d constitute a mobile signal from the anther wall to the PMCs as carriers of U-phasiRNAs (Fig.…”

Section: Discussionmentioning

confidence: 99%

Spatial distribution of three ARGONAUTEs regulates the anther phasiRNA pathway

et al. 2023

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Argonaute protein (AGO) in association with small RNAs is the core machinery of RNA silencing, an essential mechanism for precise development and defense against pathogens in many organisms. Here, we identified two AGOs in rice anthers, AGO1b and AGO1d, that interact with phased small interfering RNAs (phasiRNAs) derived from numerous long non-coding RNAs. Moreover, 3D-immunoimaging and mutant analysis indicated that rice AGO1b and AGO1d cell type-specifically regulate anther development by acting as mobile carriers of these phasiRNAs from the somatic cell layers to the germ cells in anthers. Our study also highlights a new mode of reproductive RNA silencing via the specific nuclear and cytoplasmic localization of three AGOs, AGO1b, AGO1d, and MEL1, in rice pollen mother cells.

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“…The in situ hybridization experiments were performed as previously described 70 . Briefly, the transcripts of TCP4 or STK were amplified with primer pairs listed in Supplementary Table 1 for probe preparation.…”

Section: Methodsmentioning

confidence: 99%

Arabidopsis TCP4 transcription factor inhibits high temperature-induced homeotic conversion of ovules

Lan,

Wang,

Wang

et al. 2023

Nat Commun

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Abnormal high temperature (HT) caused by global warming threatens plant survival and food security, but the effects of HT on plant organ identity are elusive. Here, we show that Class II TEOSINTE BRANCHED 1/CYCLOIDEA/ PCF (TCP) transcription factors redundantly protect ovule identity under HT. The duodecuple tcp2/3/4/5/10/13/17/24/1/12/18/16 (tcpDUO) mutant displays HT-induced ovule conversion into carpelloid structures. Expression of TCP4 in tcpDUO complements the ovule identity conversion. TCP4 interacts with AGAMOUS (AG), SEPALLATA3 (SEP3), and the homeodomain transcription factor BELL1 (BEL1) to strengthen the association of BEL1 with AG-SEP3. The tcpDUO mutant synergistically interacts with bel1 and the ovule identity gene seedstick (STK) mutant stk in tcpDUO bel1 and tcpDUO stk. Our findings reveal the critical roles of Class II TCPs in maintaining ovule identity under HT and shed light on the molecular mechanisms by which ovule identity is determined by the integration of internal factors and environmental temperature.

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Mobile ARGONAUTE 1d binds 22-nt miRNAs to generate phasiRNAs important for low-temperature male fertility in rice

Cited by 16 publications

References 56 publications

Beyond Loading: Functions of Plant ARGONAUTE Proteins

Beyond Loading: Functions of Plant ARGONAUTE Proteins

Spatial distribution of three ARGONAUTEs regulates the anther phasiRNA pathway

Arabidopsis TCP4 transcription factor inhibits high temperature-induced homeotic conversion of ovules

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