Mo-HLPs: New flocculating agents identified from Moringa oleifera seeds belong to the hevein-like peptide family

Sousa, Ana Márjory Paiva; Salles, H. O.; Oliveira, Hermógenes David de; Souza, Beatriz Blenda Pinheiro de; Filho, José de Lima Cardozo; Sifuentes, Daniel Nogoceke; Prates, Maura V.; Bloch, Carlos; Bemquerer, Marcelo P.; Egito, A. S. do

doi:10.1016/j.jprot.2020.103692

Cited by 11 publications

(6 citation statements)

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“…Four new flocculant peptides were isolated and identified from M. oleifera seeds, with the molecular masses between 4.5 and 4.8 kDa and antimicrobial activity . Lopes et al purified a protein (named MoCBP4) with antifungal activity from M.…”

Section: Introductionmentioning

confidence: 99%

“…12 Four new flocculant peptides were isolated and identified from M. oleifera seeds, with the molecular masses between 4.5 and 4.8 kDa and antimicrobial activity. 13 Lopes et al purified a protein (named MoCBP4) with antifungal activity from M. oleifera seeds and studied its antifungal mechanism of increasing cell membrane permeability. 14 Similarly, Chandrashekar et al isolated Napin-1A peptide from M. oleifera seeds with antibacterial and anticoagulant activities.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of a Novel Antimicrobial Peptide Isolated from Moringa oleifera Seed Protein Hydrolysates and Its Membrane Damaging Effects on Staphylococcus aureus

Zhao

Wang

et al. 2022

J. Agric. Food Chem.

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The present study sought to identify and characterize a novel antimicrobial peptide, named MOp2 from Moringa oleifera seed protein hydrolysates, and elucidate its potential antimicrobial effects on Staphylococcus aureus. MOp2, with the amino acid sequence of His−Val−Leu−Asp−Thr−Pro−Leu−Leu (HVLDTPLL), was characterized as a hydrophobic anionic AMP of the β-sheet structure. MOp2 exhibited negligible hemolytic activity at 2.0× MIC, suggesting its inhibitory effect on the growth of S. aureus (MIC: 2.204 mM). It maintained more than 90% of antimicrobial activity under 5% salt and about 78% of antimicrobial activity at a high temperature of 115 °C for 30 min. Protease, especially acid protease, reduced its antimicrobial activity to different extents. Moreover, MOp2 caused irreversible membrane damage to S. aureus cells by increasing the membrane permeability, resulting in the release of intracellular nucleotide pools. Additionally, molecular docking revealed that MOp2 could inhibit S. aureus growth by interacting with dihydrofolate reductase and DNA gyrase through hydrogen bonding and hydrophobic interactions. Overall, MOp2 could be a potential novel antimicrobial agent against S. aureus in food processing.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of a Novel Antimicrobial Peptide Isolated from Moringa oleifera Seed Protein Hydrolysates and Its Membrane Damaging Effects on Staphylococcus aureus

Zhao

Wang

et al. 2022

J. Agric. Food Chem.

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“…In the current study, suramin was found to bind to the C-terminal of ETD also (Figure 11C). The 2S albumin seed storage proteins (Ullah et al, 2015) and flocculating protein (Sousa et al, 2020) from M. oleifera are highly positively charged proteins. In our previous study, we had shown that these proteins bind to SARS-CoV-2 3CL M pro in-between domain II and III and restrict their moment (Ullah and Ullah, 2021).…”

Section: Differences Between Exfoliative Toxins and Other Serine Prot...mentioning

confidence: 99%

Three-Dimensional Structure Characterization and Inhibition Study of Exfoliative Toxin D From Staphylococcus aureus

et al. 2022

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The Staphylococcus aureus exfoliative toxins (ETs) are the main toxins that produce staphylococcal scalded skin syndrome (SSSS), an abscess skin disorder. The victims of the disease are usually newborns and kids, as well as grown-up people. Five ETs namely, exfoliative toxins A, B, C, D, and E have been identified in S. aureus. The three-dimensional (3D) structure of exfoliative toxins A, B, C and E is known, while that of exfoliative toxin D (ETD) is still unknown. In this work, we have predicted the 3D structure of ETD using protein modeling techniques (software used for 3D structure modeling comprising the MODELLER 9v19 program, SWISS-Model, and I-TESSER). The validation of the build model was done using PROCHECK (Ramachandran plot), ERRAT2, and Verify 3D programs. The results from 3D modeling show that the build model was of good quality as indicated by a GMQE score of 0.88 and by 91.1% amino acid residues in the most favored region of the Ramachandran plot, the ERRAT2 quality factor of 90.1%, and a verify3D score of >0.2 for 99.59% of amino acid residues. The 3D structure analysis indicates that the overall structure of ETD is similar to the chymotrypsin-like serine protease fold. The structure is composed of 13 β-strands and seven α-helices that fold into two well-defined six-strand β-barrels whose axes are roughly perpendicular to each other. The active site residues include histidine-97, aspartic acid-147, and serine-221. This represents the first structure report of ETD. Structural comparison with the other ETs shows some differences, particularly in the loop region, which also change the overall surface charge of these toxins. This may convey variable substrate specificity to these toxins. The inhibition of these toxins by natural (2S albumin and flocculating proteins from Moringa oleifera seeds) and synthetic inhibitors (suramin) was also carried out in this study. The results from docking indicate that the inhibitors bind near the C-terminal domain which may restrict the movement of this domain and may halt the access of the substrate to the active site of this enzyme. Molecular dynamic simulation was performed to see the effect of inhibitor binding to the enzyme. This work will further elucidate the structure–function relationship of this enzyme. The inhibition of this enzyme will lead to a new treatment for SSSS.

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“…Una de las partes de la planta de Moringa oleifera que posee mucha demanda son las semillas, este árbol demora, por lo general, seis meses para comenzar a producir semillas (Pérez et al, 2010), de estas, entre 35 y 45 % del peso puede llegar a estar constituido por aceite esencial, el cual contiene una gran variedad de compuestos antioxidantes y ácidos grasos de cadena corta (Zhao et al, 2019). Esta composición le otorga valor agregado por conservar su calidad, lo que contribuye a que sea empleado como emulsionante en productos farmacológicos, aderezo en la industria culinaria (Fu et al, 2021) y producción de biodiesel (Omonhinmin et al, 2020), Otros usos de las semillas de Moringa oleifera son la filtración de agua, debido a sus propiedades floculantes (Nouhi et al, 2019;Paiva Sousa et al, 2020), o la confección de tortas para la alimentación animal a partir de la masa resultante en la extracción del aceite (Afolayan et al 2020;Hansen et al, 2020). Su uso más habitual es la multiplicación de la especie, debido a las características que presentan las plantas que se obtienen a partir de reproducción sexual (mejor desarrollo radicular, foliar y crecimiento acelerado) respecto a las obtenidas por reproducción asexual (raíces cortas, crecimiento lento, dispersión de la copa) .…”

Section: Introductionunclassified

Producción de semilla de variedades de Moringa oleifera Lam según densidad de siembra y cosechas sucesivas

et al. 2022

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Introducción. Moringa oleifera Lam. es un árbol caducifolio de crecimiento perenne ontogenérico al crecimiento de las arbustivas. Moringa es el único género de la familia Moringaceae, los principales estudios de la especie olerifera se centran en la producción de forraje y muy pocos en la producción de semillas. Objetivo. Evaluar densidades de siembra y cosechas sucesivas para la producción de semillas de variedades de M. oleifera Lam, en un ecosistema degradado por la sequía estacional en Valle del Cauto, provincia Granma, Cuba. Materiales y métodos. El estudio se desarrolló del 2016 al 2018 en bancos de semillas de la Estación Experimental de Pastos y Forrajes (20º 27´ 52,1” N 76º 40’47” O) del Instituto de Investigaciones Agropecuarias “Jorge Dimitrov”, Cuba, mediante un diseño de bloques al azar en arreglo factorial con cuatro repeticiones, para un total de 64 tratamientos resultantes de la combinación entre densidad de siembra (4), variedades de M. oleifera (4) y número de cosechas (4). Las variables consideradas fueron: número de semillas por fruto, número de frutos por planta, longitud de frutos, peso de 100 semillas y rendimiento de semillas. Resultados. La variedad Criolla y Supergenius fueron las de mayor producción de semillas (p≤0,01) (0,67 y 0,71 t ha-1, respectivamente), en la segunda cosecha en julio de 2017, respecto al resto de las cosechas y variedades en estudio. La variedad Supergenius aportó los mayores valores en el número de semillas/fruto, número de frutos/planta y longitud de los frutos/planta con 18,23, 32,08 y 49,18 cm, respectivamente. Conclusión. Las variedades de M. oleifera en estudio no tuvieron diferencias en el potencial de producción de semillas, la densidad de siembra no tuvo efecto, solo la realización de cosechas sucesivas cada seis meses condicionó una diferenciación en la producción de semillas.

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Mo-HLPs: New flocculating agents identified from Moringa oleifera seeds belong to the hevein-like peptide family

Cited by 11 publications

References 54 publications

Characterization of a Novel Antimicrobial Peptide Isolated from Moringa oleifera Seed Protein Hydrolysates and Its Membrane Damaging Effects on Staphylococcus aureus

Characterization of a Novel Antimicrobial Peptide Isolated from Moringa oleifera Seed Protein Hydrolysates and Its Membrane Damaging Effects on Staphylococcus aureus

Three-Dimensional Structure Characterization and Inhibition Study of Exfoliative Toxin D From Staphylococcus aureus

Producción de semilla de variedades de Moringa oleifera Lam según densidad de siembra y cosechas sucesivas

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