MNAzyme-catalyzed nucleic acid detection enhanced by a cationic copolymer

Gao, Jueyuan; Shimada, Noritomo; Maruyama, Atsushi

doi:10.1039/c4bm00449c

Cited by 27 publications

(11 citation statements)

References 24 publications

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“…[2] DZ-based biosensors are currently employed for sensing metal ions. [3] They have also been proposed for analysis of nucleic acids [4] and other biological molecules. [5] The advantageous characteristics of DZ sensors include biocompatibility, affordable cost, versatility in design, and chemical stability (in comparison with protein and RNA sensors).…”

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confidence: 99%

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DNA Antenna Tile‐Associated Deoxyribozyme Sensor with Improved Sensitivity

et al. 2016

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Some natural enzymes increase the rate of diffusion-limited reactions by facilitating substrate flow to their active sites. Inspired by this natural phenomenon, we developed a strategy for efficient substrate delivery to a deoxyribozyme (DZ) catalytic sensor. This resulted in 3- to 4-fold increase in its sensitivity and up to 9-fold improvement of the detection limit. The reported strategy can be used to enhance catalytic efficiency of diffusion-limited enzymes and to improve sensitivity of enzyme-based biosensors.

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confidence: 99%

“…This last feature helped to maintain low background in the absence of a specific analyte, which would be impossible if an alternative structure-switching DZ sensor format (e.g. ‘catalytic molecular beacons’) [4a,b] was implemented. (iv) The tile associated BiDZ should inherit its high selectivity common to all binary (split) sensors.…”

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confidence: 99%

DNA Antenna Tile‐Associated Deoxyribozyme Sensor with Improved Sensitivity

et al. 2016

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“…This platform is assembled on exposure to target sequences and then with the catalytic activity, leads to the production of a detectable reporter, which can then be read out [121,122]. It was reported that MNAzyme modification with a cationic copolymer could increase its selectivity and activity by 250-and 2700-fold, respectively [123,124]. Considering the singleplex limitation mentioned earlier, Safdar et al developed a multiplexed DNA detection platform based on the use of MNAzyme strategy [125].…”

Section: Nanobiomaterial-based Biosensors For the Diagnosis Of Covid-19mentioning

confidence: 99%

Role of biomaterials in the diagnosis, prevention, treatment, and study of corona virus disease 2019 (COVID-19)

et al. 2021

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Recently emerged novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and the resulting corona virus disease 2019 (COVID-19) led to urgent search for methods to prevent and treat COVID-19. Among important disciplines that were mobilized is the biomaterials science and engineering. Biomaterials offer a range of possibilities to develop disease models, protective, diagnostic, therapeutic, monitoring measures, and vaccines. Among the most important contributions made so far from this field are tissue engineering, organoids, and organ-on-a-chip systems, which have been the important frontiers in developing tissue models for viral infection studies. Also, due to low bioavailability and limited circulation time of conventional antiviral drugs, controlled and targeted drug delivery could be applied alternatively. Fortunately, at the time of writing this paper, we have two successful vaccines and new at-home detection platforms. In this paper, we aim to review recent advances of biomaterial-based platforms for protection, diagnosis, vaccination, therapeutics, and monitoring of SARS-CoV-2 and discuss challenges and possible future research directions in this field.

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“…However, this was achieved so far only through complex physico-chemical modification of NAzymes (e.g. by introducing modified nucleotides or using cationic copolymers for reaction turnover enhancement [27,[35][36][37][38]), or by elevating temperature prior to the reaction [39,40]. The latter more specifically poses issues when using NAzymes not only for DNAtarget detection, but also for detection of other target molecules (e.g.…”

Section: Introductionmentioning

confidence: 99%

Re-engineering 10–23 core DNA- and MNAzymes for applications at standard room temperature

et al. 2018

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DNA-and MNAzymes are nucleic acid-based enzymes (NAzymes), which infiltrated the otherwise protein-rich field of enzymology three decades ago. The 10-23 core NAzymes are one of the most widely used and well-characterized NAzymes, but often require elevated working temperatures or additional complex modifications for implementation at standard room temperatures. Here, we present a generally applicable method, based on thermodynamic principles governing hybridization, to re-engineer the existing 10-23 core NAzymes for use at 23 °C. To establish this, we first assessed the activity of conventional NAzymes in the presence of cleavable and non-cleavable substrate at 23 °C as well as over a temperature gradient. These tests pointed towards a non-catalytic mechanism of signal generation at 23 °C, suggesting that conventional NAzymes are not suited for use at this temperature. Following this, several novel NAzyme-substrate complexes were re-engineered from the conventional ones, and screened for their performance at 23 °C. The complex with substrate and substrate-binding arms of the NAzymes shortened by four nucleotides on each terminus demonstrated efficient catalytic activity at 23 °C. This has been further validated over a dilution of enzymes or enzyme components, revealing their superior performance at 23 °C compared to the conventional 10-23 core NAzymes at their standard operating temperature of 55 °C. Finally, the proposed approach was applied to successfully re-engineer three other new MNAzymes for activity at 23 °C. As such, these re-engineered NAzymes present a remarkable addition to the field by further widening the diverse repertoire of NAzymes applications.

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MNAzyme-catalyzed nucleic acid detection enhanced by a cationic copolymer

Cited by 27 publications

References 24 publications

DNA Antenna Tile‐Associated Deoxyribozyme Sensor with Improved Sensitivity

DNA Antenna Tile‐Associated Deoxyribozyme Sensor with Improved Sensitivity

Role of biomaterials in the diagnosis, prevention, treatment, and study of corona virus disease 2019 (COVID-19)

Re-engineering 10–23 core DNA- and MNAzymes for applications at standard room temperature

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