MnAl Layered Double Hydroxide Nanoparticles as a Dual‐Functional Platform for Magnetic Resonance Imaging and siRNA Delivery

Abstract

Multifunctional nanoparticles for cancer theranosis have been widely explored for effective cancer detection and therapy. In this work, dually functionalised manganese-based layered double hydroxide nanoparticles (Mn-LDH) were examined as an effective anticancer drug/gene delivery system and for T -weighted magnetic resonance imaging (MRI) in brain cancer theranostics. Such Mn-LDH have been shown to accommodate dsDNA/siRNAs and efficiently deliver them to Neuro-2a cells (N2a). Mn-LDH have also shown high biocompatibility with low cytotoxicity. Importantly, the cell-death siRNA (CD-siRNA) delivered with Mn-LDH more efficiently kills brain cancer cells than the free CD-siRNA. Moreover, Mn-LDH can act as excellent contrast agents for MRI, with an r value of 4.47 mm s , which is even higher than that of commercial contrast agents based on Gd complexes (r =3.4 mm s ). Altogether, the high delivery efficacy and MRI contrast capability make dual-functional Mn-LDH potential bimodal agents for simultaneous cancer diagnosis and therapy.

2D Nanostructured Metal Hydroxides with Gene Delivery and Theranostic Functions; A Comprehensive Review

“…So attempts were made to immobilize gene molecules with low concentration only on the surface of LDH nanoparticles in an aqueous solution as shown in Figure (D). After 2004, many efforts have also been made to immobilize newly developed gene fragments onto LDH nanoparticles simply by mixing LDH nanoparticles with several types of genes, including plasmid DNA,,, ssDNA/dsDNA, and siRNA, as represented in Table .…”

“…Recently, many efforts have been made to explore gene‐LDH hybrid materials functionalized with imaging moieties such as gadolinium, manganese, Red siSNA546, and Cy3 tagged DNA for theranostics (Table ) ,,. Yoo et al .…”

“…Zuo et al . were successfully prepared dually functionalized manganese‐based LDH nanoparticles (Mn–LDHs), and examined it not only as an effective gene delivery system, but also as a T1 weighted MR imaging modality for brain cancer theranostics . And it was found that Mn–LDHs could play a role as an excellent contrast agent for MR imaging, with the relaxation rate ( r 1 ) value of 4.47 mM −1 s −1 , even better than commercially available Gd‐based complex contrast agent ( r 1 =3.4 mM −1 s −1 ) …”

Discovery of Layered Indium Hydroxide via a Hydroperoxyl Anion Coordinated Precursor at Room Temperature

“…Layered metal hydroxides (LMHs), including layered single metal hydroxides (LSHs) and layered double hydroxides (LDHs), belong to an important category of 2D materials. LMHs and their ultrathin forms have been studied extensively in many fields such as catalysis, magnetism, energy storage, and conversion due to the flexible tunability of the metal ions . However, both LSHs and LDHs have only one structure type reported up to now, that is, brucite or brucite‐like metal‐hydroxyl layers, composed of edge shared M(OH) 6 octahedra .…”

“…Layered metal hydroxides (LMHs), including layered single metal hydroxides( LSHs) and layered double hydroxides (LDHs), belong to an important category of 2D materials.LMHs and their ultrathin forms have been studied extensively in many fields such as catalysis, magnetism, energy storage, and conversion due to the flexible tunability of the metal ions. [1][2][3][4][5][6][7][8][9][10] However,b oth LSHs and LDHs have only one structure type reported up to now,t hat is, bruciteo rb rucite-like metal-hydroxyl layers, composed of edge shared M(OH) 6 octahedra. [11] The structure of the material has ag reat influence on the properties;t he range of LMHs and their applications can be expanded if layersw ith an ew structure type can be synthesized.In(OH) 3 ,p ossessing only one three-dimensional structure,i s not only af unctional material, but also an important precursor for In 2 O 3 .G reat attention has been attracted to In(OH) 3 because of its potential applicationsi nv arious fields including gas sensing, [12][13][14] electronic devices, [15,16] and photocatalysis.…”