MMP-9 reinforces radiation-induced delayed invasion and metastasis of neuroblastoma cells through second-signaling positive feedback with NFκB via both ERK and IKK activation

Somasundaram, Dinesh Babu; Aravindan, Sheeja; Major, Ryan; Natarajan, Mohan; Aravindan, Natarajan

doi:10.1007/s10565-021-09663-4

Cited by 4 publications

(2 citation statements)

References 82 publications

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“…RNA-seq results further suggested that the signaling pathway inactivated by overexpression of NBASP involved ERK signaling, which was mediated by FABP5. Previous studies reported that active ERK was usually associated with increased malignancy during the progression of NB [38][39][40]. Although we showed that FABP5 interacted with NBASP and inhibited the ERK pathway, how these two proteins cooperatively contribute to ERK signaling still needs further study.…”

Section: Discussionmentioning

confidence: 60%

Small Protein NBASP Encoded by lncRNA FAM201A Decreases Neuroblastoma Progression via FABP5

Gao

Chen³

et al. 2022

Preprint

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Increasing evidence indicates that long non-coding RNA (lncRNA) is one of the most important RNA regulators in the pathogenesis of neuroblastoma (NB). However, limited studies have addressed the possibility that lncRNAs possess the ability of encoding functional peptides, which could affect the initiation and development of tumors by participating in signal pathway transduction, immune regulation, and tumor metabolism. Here, we found that downregulation of lncRNA FAM201A was associated with NB malignant progression and a poor prognosis. A variety of gain- and loss-of-function studies elucidated the anti-tumor effects of lncFAM201A on the regulation of growth and metastasis of NB cells. Surprisingly, combined with database predictions and experimental verifications, we found that significantly down-regulating lncRNA FAM201A in NB identified the ability to encode the tumor-suppressing micropeptide, NBASP, which negatively regulated the expression of FABP5. Mechanistically, our transcriptomics analyses confirmed that the regulatory role between NBASP and FABP5 was mediated by activation of the ERK pathway. In conclusion, our findings revealed that NBASP encoded by lncRNA FAM201A played a tumor-suppressor role in NB carcinogenesis via down-regulating FABP5 to inactivate the ERK pathway. These results extended our understanding of the relationship of lncRNA-encoded functional peptides and plasticity of tumor progression.

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Section: Discussionmentioning

confidence: 60%

Small Protein NBASP Encoded by lncRNA FAM201A Decreases Neuroblastoma Progression via FABP5

Gao

Chen³

et al. 2022

Preprint

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“…MMP9 is involved in various tumor cell cellular events, including growth, differentiation, invasion, metastasis, regulation of tumor angiogenesis, and immune surveillance. In addition, MMP9 is closely related to the occurrence and development of various tumors, [6][7][8] including rectal cancer, [9] neuroblastoma, [10] papillary thyroid carcinoma, [11] bladder cancer, [12] non-small cell lung cancer. [13] As an oncogene, MMP9 plays an important regulatory role in the occurrence and development of various tumors, suggesting that MMP9, especially MMP9 after electric field stimulation, could be a potential tumor biomarker or therapeutic target.…”

Section: Introductionmentioning

confidence: 99%

Molybdenum disulfide field-effect transistor biosensor for ultrasensitive detection of matrix metallopeptidase 9 (MMP9) gene

Liu

Feng

et al. 2022

Preprint

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Background Biosensors are an emerging field in biomedical diagnostics. In the field of biosensors, field-effect transistor (FET)-based biosensors are widely used in clinical, environmental, and food analysis fields because of their high sensitivity, specificity, and real-time detection. Matrix metallopeptidase 9 (MMP9) is a gene closely related to various diseases, which is considered to have a predictive role in disease diagnosis and qualitative treatment. Methods In this study, an all-solid-state, ultrasensitive FET biosensor based on monolayer molybdenum disulfide (MoS2) was designed and constructed. We synthesized a series of MMP9-targeted probes and obtained the optimal hybridization conditions (including optimal hybridization temperature, hybridization concentration, and hybridization time) for the MoS2 FET biosensors. Results The data show that this MoS2 FET biosensor detects the MMP9 gene at a minimum of 3.86 pM with promising selectivity, specificity, and reproducibility. Conclusion The MoS2 FET biosensor detects the MMP9 gene at a minimum of 3.86 pM with promising selectivity, specificity, and reproducibility. Our work not only provides a potential strategy for the diagnosis and treatment of MMP9-related diseases, but also is helpful for the design of nanodevices, the development of portable diagnostic devices, and the implementation of personalized medicine.

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FAM201A encodes small protein NBASP to inhibit neuroblastoma progression via inactivating MAPK pathway mediated by FABP5

Gao

Chen

et al. 2023

Commun Biol

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Increasing evidence indicates that long non-coding RNA (lncRNA) is one of the most important RNA regulators in the pathogenesis of neuroblastoma (NB). Here, we found that FAM201A was low expressed in NB and a variety of gain and loss of function studies elucidated the anti-tumor effects of FAM201A on the regulation of proliferation, migration and invasion of NB cells. Intriguingly, we identified the ability of FAM201A to encode the tumor-suppressing protein, NBASP, which interacted with FABP5 and negatively regulated its expression. In vivo assays also revealed NBASP repressed NB growth via inactivating MAPK pathway mediated by FABP5. In conclusion, our findings demonstrated that NBASP encoded by FAM201A played a tumor-suppressor role in NB carcinogenesis via down-regulating FABP5 to inactivate the MAPK pathway. These results extended our understanding of the relationship of lncRNA-encoded functional peptides and plasticity of tumor progression.

show abstract

MMP-9 reinforces radiation-induced delayed invasion and metastasis of neuroblastoma cells through second-signaling positive feedback with NFκB via both ERK and IKK activation

Cited by 4 publications

References 82 publications

Small Protein NBASP Encoded by lncRNA FAM201A Decreases Neuroblastoma Progression via FABP5

Small Protein NBASP Encoded by lncRNA FAM201A Decreases Neuroblastoma Progression via FABP5

Molybdenum disulfide field-effect transistor biosensor for ultrasensitive detection of matrix metallopeptidase 9 (MMP9) gene

FAM201A encodes small protein NBASP to inhibit neuroblastoma progression via inactivating MAPK pathway mediated by FABP5

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