MMP-9 regulates CX3CL1/CX3CR1 in the early phase of neuropathic pain in chronic sciatic nerve constriction injury (CCI) rats

Zhao, Li; Song, Congzhong; Huang, Yunpeng; Lei, Weiping; Sun, Jiao

doi:10.21037/apm-20-1078

Cited by 13 publications

(9 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fractalkine receptor, CX3CR1, a marker of peripheral monocytic cells and microglia, was increased in the ipsilateral DRG in a spared nerve injury-induced neuropathic pain model [ 67 ]. In addition, the involvement of MMP-9 in the early phase of neuropathic pain in CCI rats was related to the upregulation of C-X3-C Motif Chemokine Ligand 1 (CX3CL1)/CX3CR1 [ 68 ]. Our results showed that CJE, (−)-epicatechin, and rutin treatment significantly reduced the upregulation of gene expressions.…”

Section: Discussionmentioning

confidence: 99%

The Antinociceptive Potential of Camellia japonica Leaf Extract, (−)-Epicatechin, and Rutin against Chronic Constriction Injury-Induced Neuropathic Pain in Rats

2022

View full text Add to dashboard Cite

Neuropathic pain is caused by a lesion or disease of the somatosensory nervous system. Currently, prescribed treatments are still unsatisfactory or have limited effectiveness. Camellia japonica leaves are known to have antioxidant and anti-inflammatory properties.; however, their antinociceptive efficacy has not yet been explored. We examined the antinociceptive efficacy and underlying mechanism of C. japonica leaf extract (CJE) in chronic constriction injury (CCI)-induced neuropathic pain models. To test the antinociceptive activity of CJE, three types of allodynia were evaluated: punctate allodynia using von Frey filaments, dynamic allodynia using a paintbrush and cotton swab, and cold allodynia using a cold plate test. CCI rats developed neuropathic pain representing increases in the three types of allodynia and spontaneous pain. In addition, CCI rats showed high phosphorylation levels of mitogen-activated protein kinases (MAPKs), transcription factors, and nociceptive mediators in dorsal root ganglion (DRG). The ionized calcium-binding adapter molecule 1 levels and neuroinflammation also increased following CCI surgery in the spinal cord. CJE and its active components have potential antinociceptive effects against CCI-induced neuropathic pain that might be mediated by MAPK activation in the DRG and microglial activation in the spinal cord. These findings suggest that CJE, (−)-epicatechin, and rutin could be novel candidates for neuropathic pain management.

show abstract

Section: Discussionmentioning

confidence: 99%

The Antinociceptive Potential of Camellia japonica Leaf Extract, (−)-Epicatechin, and Rutin against Chronic Constriction Injury-Induced Neuropathic Pain in Rats

2022

View full text Add to dashboard Cite

show abstract

“…MMP9 is significantly associated with the onset of neuropathic pain rather than its maintenance (28). However, specific molecular mechanisms related to MMP9 and DRG inflammation pain are lacking; this mechanism should be further investigated, as MMP9 may be a novel candidate biomarker for DRG inflammation pain.…”

Section: Discussionmentioning

confidence: 99%

Identification of key candidate genes in local dorsal root ganglion inflammation by integrated bioinformatics analysis

et al. 2021

View full text Add to dashboard Cite

The purpose of the present study was to identify potential markers of local dorsal root ganglion (DRG) inflammation to aid diagnosis, treatment and prognosis evaluation of DRG pain. A localized inflammation of the DRG (LID) rat model was used to study the contribution of inflammation to pain. The dataset GSE38859 was obtained from the Gene Expression Omnibus database. Pre-treatment standardization of gene expression data for each experiment was performed using the R/Bioconductor Limma package. Differentially expressed genes (DEGs) were identified between a LID model and a sham surgery control group. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of DEGs and gene set enrichment analysis (GSEA) were carried out using the 'clusterProfiler' package in R. Using the Search Tool for Retrieval of Interacting Genes, a protein-protein interaction network was constructed and visualized. Candidate genes with the highest potential validity were validated using reverse transcription-quantitative PCR and western blotting. In total, 66 DEGs were enriched in GO terms related to inflammation and the immune response processes. KEGG analysis revealed 14 associated signaling pathway terms. Protein-protein interaction network analysis revealed 9 node genes, 3 of which were among the top 10 DEGs. Matrix metallopeptidase 9, chemokine CXCL9, and complement component 3 were identified as key regulators of DRG inflammatory pain progression.

show abstract

“…During nervous system development and following injury, MMPs have been increasingly recognized as playing vital roles [24]. For example, MMP-9 was associated with the early process of neuropathic pain in CCI rats [25]; and in peripheral nerve regeneration, MMP-7 promoted myelin sheath formation and Schwann cell migration [15]. As the smallest member of MMPs, MMP-7 plays vital roles in many physiological processes [26].…”

Section: Discussionmentioning

confidence: 99%

The Roles of Matrix Metalloproteinase 7 in Spinal Cord Ischemia- Reperfusion Injury in Rats

Fang¹,

Chen²,

Wang³

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: Neuronal survival after spinal cord ischemia-reperfusion injury (SCII) is a major factor affecting the motor function. Recently, matrix metalloproteinase-7 (MMP-7) plays an important role in a variety of diseases, but the specific role of MMP-7 in SCII remains unclear. This paper aims to further investigate the role of MMP7 in SCII.Methods: We built the SCII model by clipping the aortic arch for 14 minutes. The RNA and protein expression of MMP-7 was detected by Western blot and real-time polymerase chain reaction (PCR) during the groups. The co-localization of major cell types and MMP-7 in the spinal cords was detected by Immunofluorescence staining. To further study the specific mechanism, rats were intrathecally pretreated with si-MMP7 or negative control (NC) siRNA 3 days before clipping the aortic arch for 14 minutes. The Tarlov criteria and Haematoxylin and eosin staining were used to detect neurological function and histological assessment. Western blot, PCR and Immunofluorescence staining were used to evaluate the effect of silencing MMP-7.Results: The expression of MMP-7 RNA and protein increased both at 12 h and 24 h after SCII. At 24 h after SCII, the expression of MMP-7 RNA reached its maximum amount. Compared with the sham group, MMP-7 fluorescent expression in the SCII group greatly enhanced, and MMP-7 fluorescence was mainly co-expressed with neuronal nuclei (NeuN) fluorescence. The Tarlov scores and number of intact neurons were increased by pre-treatment with si-MMP7. Pre-treatment with si-MMP7 could also decrease the expression of MMP-7, Interleukin-1β (IL-1β) and cleaved caspase-3 and reduce the MMP-7 expression in neurons. Conclusions: Silencing MMP-7 protected the rats against SCII by inhibiting the neuroapoptosis and inflammation. Silencing MMP-7 could be a hopeful therapeutic treatment after SCII.

show abstract

MMP-9 regulates CX3CL1/CX3CR1 in the early phase of neuropathic pain in chronic sciatic nerve constriction injury (CCI) rats

Cited by 13 publications

References 14 publications

The Antinociceptive Potential of Camellia japonica Leaf Extract, (−)-Epicatechin, and Rutin against Chronic Constriction Injury-Induced Neuropathic Pain in Rats

The Antinociceptive Potential of Camellia japonica Leaf Extract, (−)-Epicatechin, and Rutin against Chronic Constriction Injury-Induced Neuropathic Pain in Rats

Identification of key candidate genes in local dorsal root ganglion inflammation by integrated bioinformatics analysis

The Roles of Matrix Metalloproteinase 7 in Spinal Cord Ischemia- Reperfusion Injury in Rats

Contact Info

Product

Resources

About