MMP-2, MMP-9, and iNOS Expression in Human Dental Pulp Subjected to Orthodontic Traction

Leone, Angelo; Mauro, Annamaria; Spatola, Giovanni Francesco; Provenzano, Salvatore; Caradonna, Carola; Gerbino, Aldo; Buscemi, M

doi:10.2319/110308-557r.1

Cited by 8 publications

(6 citation statements)

References 23 publications

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“…Furthermore, these data also demonstrated that high iNOS expression was dramatically associated with pathology T ( p =0.002) and pathology M ( p =0.029), which serve as important prognostic markers for patients with ICC. iNOS-positive expression in ICC tended to be correlated with Wip1-positive/MMP-2-positive or MMP-9-positive ( p =0.019, p =0.028, p =0.046, respectively), which is in agreement with our previous and other studies 16,20–23…”

Section: Resultssupporting

confidence: 92%

<p>iNOS is associated with tumorigenicity as an independent prognosticator in human intrahepatic cholangiocarcinoma</p>

Liu¹,

Jiang²,

Huang³

et al. 2019

CMAR

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BackgroundInducible nitric oxide synthase (iNOS) has supposed to implicate in inflammation, infection, liver cirrhosis, and neoplastic diseases. This study was designed to explore the biological and clinical function of iNOS in intrahepatic cholangiocarcinoma (ICC).MethodsRT-PCR (Real-time quantitative PCR) and immunohistochemical staining were used to analyze the expression of iNOS in ICC and adjacent tissues. CCK8, transwell assays, flow cytometry were conducted to detect the proliferation, apoptosis, cell cycle. Western blotting was performed to detect the expression of target proteins. Multivariate analyses were conducted to analysis associates between clinicopathological values and survival.ResultsWe found that levels of iNOS mRNA and protein were dramatically increased in ICC samples and positively correlated with complicated bile duct stone, differentiation, pathology T, pathology M, Wip1, MMP-2, and MMP-9. iNOS expression was significantly correlated with the poor survival of ICC patients. Furthermore, iNOS was high expression in ICC cell lines (QBC-939, ICC-9810, SSP-25) compare with human normal biliary epithelium cell line (HIBEpic); both iNOS knockdown and iNOS inhibitor (1400 W) suppressed cell proliferation, invasion, and migration though nitric oxide production in ICC cells. Down-regulation of iNOS also induced G0/G1 cell cycle arrest and ICC cell apoptosis. Moreover, iNOS knockdown treatment significantly decreased Wip1, MMP-9, and MMP-2 gene expression.ConclusionLowly expressed iNOS-inhibited proliferation yet promoted apoptosis of ICC cells. Our data show targeted inhibition of iNOS in ICC may have therapeutic value.

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Section: Resultssupporting

confidence: 92%

<p>iNOS is associated with tumorigenicity as an independent prognosticator in human intrahepatic cholangiocarcinoma</p>

Liu¹,

Jiang²,

Huang³

et al. 2019

CMAR

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“…Eighteen studies evaluated the occurrence of histomorphological changes in the pulp after different forces: intrusion; 4,11-14 extrusion; 13,[15][16][17][18] tipping movement; 19 conventional orthodontic movement; 3,[20][21][22][23] or rapid maxillary expansion (RME). [24][25][26] Fourteen studies evaluated the expression of tissue factors after orthodontic force application, including methionineenkephalin (ME), [27][28][29] calcitonin gene-related peptide (CGRP), 27,30 substance P (SP), 27,28 matrix metalloproteinase (MMP) 2 and 9, 6,31 beta-Endorphin, 27 c-Fos, 6 caspases 3 and 9, 20 proliferating cell nuclear antigen (PCNA), 20 heat shock protein-60 (HSP60), 20 macrophageal nitric oxide synthase (iNOS), 31,32 neural nitric oxide synthase (nNOS), 32 aspartate aminotransferase (AST), [33][34][35][36][37] and alkaline phosphatase (AP). 34…”

Section: Study Selection and Characteristicsmentioning

confidence: 99%

Effect of orthodontic force on dental pulp histomorphology and tissue factor expression:

Vitali¹,

Cardoso²,

Mello³

et al. 2021

The Angle Orthodontist

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Objectives To evaluate the effects of orthodontic force on histomorphology and tissue factor expression in the dental pulp. Materials and Methods Two reviewers comprehensively and systematically searched the literature in the following databases: Latin American and Caribbean Health Sciences, Embase, Cochrane, PubMed, Scopus, Web of Science, and Grey literature (Google Scholar, OpenGrey, and ProQuest) up to September 2020. According to the Population, Intervention, Comparison, Outcomes, Studies criteria, randomized clinical trials (RCTs) and observational studies that evaluated the effects of orthodontic force on dental pulp were included. Case series/reports, laboratory-based or animal studies, reviews, and studies that did not investigate the association between orthodontic force and pulpal changes were excluded. Newcastle-Ottawa Scale and Cochrane risk-of-bias tool were used to assess the risk of bias. The overall certainty level was evaluated with the Grading of Recommendations Assessment, Development and Evaluation tool. Results 26 observational studies and five RCTs were included. A detailed qualitative analysis of articles showed a wide range of samples and applied methodologies concerning impact of orthodontic force on the dental pulp. The application of orthodontic force seems to promote several pulpal histomorphological changes, including tissue architecture, cell pattern, angiogenesis, hard tissue deposition, inflammation, and alteration of the expression levels of 14 tissue factors. Conclusions Although the included articles suggest that orthodontic forces may promote histomorphological changes in the dental pulp, due to the very low-level of evidence obtained, there could be no well-supported conclusion that these effects are actually due to orthodontic movement. Further studies with larger samples and improved methods are needed to support more robust conclusions.

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“…184,185 However, iNOS expression (typical of inflammatory conditions) in pulp subjected to orthodontic forces was present but had no obvious change compared with control dental pulp, which could be explained as a response to extractive stresses. 186 Moreover, mechanical irritants can provoke a range of inflammatory responses in dental pulp, such as the production of diverse cytokines and chemokines related with immune responses. 6,184 Another illuminating case is that occlusal forces can promote periodontal ligament healing through inducing NOS expression and NO production.…”

Section: Nos Activities and Mechanical Stressmentioning

confidence: 99%

Nitric Oxide in Dental Pulp Tissue: From Molecular Understanding to Clinical Application in Regenerative Endodontic Procedures

2020

Tissue Engineering Part B: Reviews

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Nitric oxide (NO), which is synthesized by the enzyme NO synthase (NOS), is a versatile endogenous molecule with multiple biological effects on many tissues and organs. In dental pulp tissue, NO has been found to play multifaceted roles in regulating physiological activities, inflammation processes, and tissue repair events, such as cell proliferation, neuronal degeneration, angiogenesis, and odontoblastic differentiation. However, there is a deficiency of detailed discussion on the NO-mediated interactions between inflammation and reparative/ regenerative responses in wounded dental pulp tissue, which is a central determinant of ultimate clinical outcomes. Thus, the purpose of this review is to outline the current molecular understanding on the roles of Janusfaced molecule NO in dental pulp physiology, inflammation, and reparative activities. Based on this knowledge, advanced physicochemical techniques designed to manipulate the therapeutic potential of NOS and NO production in endodontic regeneration procedures are further discussed.

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MMP-2, MMP-9, and iNOS Expression in Human Dental Pulp Subjected to Orthodontic Traction

Abstract: The hypothesis is rejected. The reduction of expression of those proteins revealed a time-dependent relationship.

Cited by 8 publications

References 23 publications

<p>iNOS is associated with tumorigenicity as an independent prognosticator in human intrahepatic cholangiocarcinoma</p>

<p>iNOS is associated with tumorigenicity as an independent prognosticator in human intrahepatic cholangiocarcinoma</p>

Effect of orthodontic force on dental pulp histomorphology and tissue factor expression:

Nitric Oxide in Dental Pulp Tissue: From Molecular Understanding to Clinical Application in Regenerative Endodontic Procedures

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