MMP-2 assay within the hybrid layer created by a two-step etch-and-rinse adhesive: Biochemical and immunohistochemical analysis

Mazzoni, Annalisa; Carrilho, Marcela Rocha de Oliveira; Papa, Verónica; Tjäderhane, Leo; Gobbi, Pier Giorgio; Nucci, Cesare; Lenarda, Roberto Di; Mazzotti, Giovanni; Tay, Franklin R.; Pashley, David H.; Breschi, Lorenzo

doi:10.1016/j.jdent.2011.04.004

Cited by 59 publications

(51 citation statements)

References 33 publications

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“…One of the most important mechanisms is considered to be the degradation of exposed collagen of the dentin hybrid layers 4,[7][8][9] . Host derived matrix metalloproteinases (MMPs) were proven to be a key factor in hydrolyzing the exposed collagen [10][11][12][13][14][15] . As chlorhexidine possesses desirable MMP-inhibitory properties 16) and outstanding substantivity to human dentin 17,18) , it has been well studied as a MMP-inhibitor to prevent dentin bond degradation 13,14,[19][20][21][22][23][24][25][26] .…”

Section: Introductionmentioning

confidence: 99%

“…Host derived matrix metalloproteinases (MMPs) were proven to be a key factor in hydrolyzing the exposed collagen [10][11][12][13][14][15] . As chlorhexidine possesses desirable MMP-inhibitory properties 16) and outstanding substantivity to human dentin 17,18) , it has been well studied as a MMP-inhibitor to prevent dentin bond degradation 13,14,[19][20][21][22][23][24][25][26] . Recent studies revealed that cysteine cathepsins are also present in intact and carious dentin which may also contribute to the breakdown of the exposed collagen in dentin hybrid layers 27,28) , and that chlorhexidine can also inhibits the activity of dentin cysteine cathepsins 29) .…”

Section: Introductionmentioning

confidence: 99%

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Pre-treatment of radicular dentin by self-etch primer containing chlorhexidine can improve fiber post bond durability

Zhou¹,

Yang²,

Chen³

et al. 2013

Dent. Mater. J.

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We evaluated whether the pre-treatment of radicular dentin by ED Primer containing different concentrations of chlorhexidine can improve the bond durability of fiber post to radicular dentin. Experimental ED primers containing different concentrations of chlorhexidine (0%, 0.5% and 1.0%) were prepared. Thirty extracted maxillary anterior teeth were divided into 3 groups, each group corresponding to different chlorhexidine concentrations. Fiber posts were cemented in endodontically treated teeth with experimental ED primers and Panavia F. The bonded teeth were transversally sectioned into six slices and then were processed for thin slice push-out test 24 h later or after 18-months water storage. Eighteen-month storage resulted in significant bond strength reduction of all groups (p<0.05). The bond strength reduction of 1.0% group was significantly lower than that of control group and 0.5% group (p<0.05). In conclusion, the incorporation of 1.0% chlorhexidine into ED primer can extend the bond longevity of fiber post to radicular dentin.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pre-treatment of radicular dentin by self-etch primer containing chlorhexidine can improve fiber post bond durability

Zhou¹,

Yang²,

Chen³

et al. 2013

Dent. Mater. J.

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“…Nos procedimentos de união utilizando adesivos convencionais, o adesivo, comumente, não penetra em toda a extensão do condicionamento, resultando em fibrilas colágenas expostas na base da camada híbrida 14,20 . Essa camada de fibrilas colágenas desprotegidas pode ser hidrolisada ou degradada por enzimas endógenas da dentina, reduzindo a resistência da união 21,22 . Assim, apesar de os diferentes condicionadores avaliados neste estudo terem proporcionado resistência de união imediata semelhante, diferenças em longo prazo podem ser observadas.…”

Section: Discussionunclassified

Influência da marca do condicionador ácido na resistência de união da resina composta à dentina

Passos

Santos

Rodrigues

et al. 2012

Rev. odontol. UNESP

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ResumoIntrodução: Apesar da importância da etapa de condicionamento ácido nos procedimentos adesivos, pouco se sabe sobre o efeito da marca do condicionador ácido sobre a união do adesivo à dentina. Objetivo: Este estudo avaliou o efeito de diferentes condicionadores ácidos na união da resina composta à dentina. Material e método: Vinte e cinco incisivos bovinos tiveram a coroa dividida em duas hemissecções. As superfícies vestibulares foram desgastadas com lixa carbeto de silício (SiC) #180 até a exposição de dentina, seguida por lixas SiC #600 para padronização da smear layer. As amostras foram aleatoriamente alocadas em cinco grupos (n = 10), de acordo com marca do condicionador ácido: 3M ESPE, Ultradent, Dentsply, Villevie e DFL. Apenas um sistema de união convencional de dois passos (Ambar, FGM) foi utilizado. Matrizes de silicone foram utilizadas na confecção de três cilindros de resina composta em cada amostra. Após 24 horas, os cilindros foram testados sob carga de cisalhamento até a falha. Os dados foram analisados com ANOVA a um critério e teste de Tukey (α = 0,05). O modo de falha foi avaliado em microscopia óptica e os dados submetidos ao Teste Qui-quadrado (α = 0,05). Resultado: Não houve diferença estatística na resistência de união obtida entre os condicionadores ácidos avaliados. Houve predomínio de falhas adesivas, exceto para os condicionadores da Dentsply e DFL, que demonstraram similaridade entre falhas adesivas e mistas. Conclusão: As diferentes marcas de condicionadores ácidos avaliados não influenciaram a resistência de união de resina composta à dentina.Descritores: Adesivos dentinários; resistência ao cisalhamento; ataque ácido dentário. AbstractIntroduction: Despite the importance of the acid etching step on bonding procedures, little is known about the acid etchant brand effect on bond strength of adhesive to dentin. Objective: The aim of this study was to evaluate the effect of different acid etchants at the bond strength of composite resin to dentine. Material and method: Twenty five bovine incisors had their crown sectioned in two hemi-sections. The buccal surfaces were grounded with #180 silicon carbide (SiC) abrasive paper to exposure of dentin, followed by #600 SiC paper to standardize the smear layer. The samples were randomly allocated into five groups (n = 10) according to acid etchant brands: 3M ESPE, Ultradent, Dentsply, Villevie and DFL.Only a two-step etch-and-rinse adhesive system (Ambar, FGM) was used. Silicon molds were used to build-up three resin composite cylinder per sample. After 24 hours, cylinders were submitted to shear load until the failure. Data were analyzed by one way ANOVA and Tukey`s test (α = 0.05). The failure mode was evaluated under optical microscopy and the data were submitted to Chi-square test (α = 0.05). Result: There was no statistical difference on bond strength obtained between the acid etchants evaluated. There was predominance of adhesive failures, except for etchants of Dentsply and DFL that showed similarity between mixed and adhesive fail...

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“…sostienen que el uso de inhibidores de las MMP no tóxicos como la CHX, es un paso adicional en los sistemas adhesivos en orden a aumentar la longevidad de las restauraciones adhesivas. 146 Aunque, este esquema presenta detractores, recientemente Lührs y col. observaron en el laboratorio que los inhibidores de la acción de las MMP no son capaces de prevenir la disminución de la resistencia a la microtracción después de envejecidos los especímenes y que adicionalmente no mejora la longevidad cuando es utilizado en resinas compuestas de cementación. 147 Los resultados en general son promisorios pero necesariamente se deben esperar estudios in vivo a largo plazo en ensayos randomizados que permitan valorar la exacta dimensión de estas investigaciones.…”

Section: Improving the Strength Of Collagen Fibers 4a Mmp Inhibitorsunclassified

“…146 Although this scheme has detractors, recently Luhrs et al conducted laboratory analysis noting that the action of MMP inhibitors fails to prevent the decreased micro-traction strength after their samples had been aged; in addition, it does not improve longevity when used in cementing composite resins. 147 These results are promising but in vivo randomized trials are needed in the long term in order to assess the exact dimension of these investigations.…”

Section: 148mentioning

confidence: 99%

Aumento de longevidad de restauraciones de resinas compuestas y de su unión adhesiva. Revisión de tema

Moncada

Vildósola

Fernández

et al. 2015

Rev Fac Odontol Univ Antioq

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ABSTRACT. Introduction: the goal of this literature review is to analyze the findings included in the literature concerning different alternatives to increase longevity of resin-based composite restorations and their adhesive bond. Method: bibliographies in the EBSCO database (Elton B StephensCompany) were reviewed using the following key words: "composite repair bond strength"; "restorations sealing"; composite restorations longevity"; "restorations refurbishment"; "composite bond strategy"; "dental adhesive collagen cross linking"; "proanthocyanidin dentin bond strength"; "multiple layer dentin bond strength", and "dentin INTRODUCCIÓNLas restauraciones dentales de resinas compuestas (RC) presentan vida útil limitada en el tiempo siendo la presencia de lesiones de caries en sus márgenes la principal causa de fracaso, en menor proporción, las fracturas dentarias o de las restauraciones, deterioros marginales, sensibilidades dentarias, perdidas de relaciones de contacto, manchas o modificaciones de color entre otras. 1-8El tiempo que funcionan las restauraciones es un elemento importante para la toma de decisiones clínicas, la evidencia de la práctica clínica revela que sobre el 60% del tiempo (rango 50% a 75%) el odontólogo lo ocupa en el reemplazo de restauraciones fracasadas 9 adicionalmente la mayoría de los pacientes desconoce la vida útil de sus restauraciones y para los servicios dentales habitualmente no es un parámetro considerado integralmente en sus presupuestos.En general los reportes de expectativas de vida de las restauraciones presentan discrepancias en su estimación, dada las diferencias en el diseño de los estudios, los criterios utilizados para la selección de casos, determinación del éxito o fracaso y la estimación de sobrevida, entre otros. Con respecto al diseño de estos estudios de longevidad, se basan en estudios prospectivos y retrospectivos, donde solo en algunos se menciona la calibración de los clínicos. Los estudios prospectivos presentan menores distorsiones porque colectan datos en estudios de diseño controlado y observan las variables consistentemente en el tiempo, sin embargo, ellos requieren muchos años para lograr suficiente validación clínica y existe la posibilidad de sesgo relacionado con el operador o el paciente. Los estudios retrospectivos presentan la ventaja de ser realizados en corto tiempo, ser de menor costo, por otro lado, presentan mayor riesgo de incorporar inexactitudes por omisión. En cambio, el estudio prospectivo presenta análisis de sobrevida de mayor exactitud y el riesgo de inexactitudes puede ser compensado incluyendo casos control donde el tiempo de fracaso no puede ser intervenido.6, 10-13 Varias metodologías han sido utilizadas para la evaluación de la calidad de las restauraciones, sin embargo el adoptado

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MMP-2 assay within the hybrid layer created by a two-step etch-and-rinse adhesive: Biochemical and immunohistochemical analysis

Cited by 59 publications

References 33 publications

Pre-treatment of radicular dentin by self-etch primer containing chlorhexidine can improve fiber post bond durability

Pre-treatment of radicular dentin by self-etch primer containing chlorhexidine can improve fiber post bond durability

Influência da marca do condicionador ácido na resistência de união da resina composta à dentina

Aumento de longevidad de restauraciones de resinas compuestas y de su unión adhesiva. Revisión de tema

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