MMP-1 and MMP-2 in the Cervix Uteri in Different Steps of Malignant Transformation—An Immunohistochemical Study

Brummer, Oliver; Böhmer, Gerd; Hollwitz, Bettina; Flemming, Peer; Petry, Karl Ulrich; Kühnle, H.

doi:10.1006/gyno.2001.6413

Cited by 62 publications

(65 citation statements)

References 23 publications

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“…During the last decades progress in research on enzyme activities showed the potential significance of MMP-2 and MMP-9 in the progression of cervical uterine cancer suggesting their prognostic value (14,15,(51)(52)(53)(54)(55)(56)(57)(58)(59)(60)(61)(62)(63)(64)(65). Sheu et al demonstrated that MMP-2 and MMP-9 were overexpressed in >90% of squamous cell carcinomas (SCC) and 83-100% of HSIL, but were less frequently expressed in LSIL and normal squamous epithelium (13%).…”

Section: Mmps In Uterine Cervical Neoplasmmentioning

confidence: 99%

Uterine cervical carcinoma: Role of matrix metalloproteinases (Review)

Libra¹

2009

Int J Oncol

115

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Abstract.Epidemiological and experimental studies have provided evidence that human papillomavirus (HPV) infection is a main player in the development of uterine cervical neoplasms. Migration of cancer cells from the origin tissue to surrounding or distant organs is essential for tumor progression. Many studies of tumor invasion and metastases have focused on the degradation of the extracellular matrix where matrix metalloproteinases (MMPs) play a central role. Two of these enzymes, MMP-2 and MMP-9, have been correlated with the processes of tumor cell invasion and metastasis in human cancers, including uterine neoplasms. It has been shown that the up-regulation of MMPs is associated with progression of cervical uterine neoplasms. This review describes the current understanding of MMP-2 and MMP-9 expression and activity in pre-cancer and cancer lesions of cervical uterine, which may open new strategies for diagnostic and therapeutic interventions.

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Section: Mmps In Uterine Cervical Neoplasmmentioning

confidence: 99%

Uterine cervical carcinoma: Role of matrix metalloproteinases (Review)

Libra¹

2009

Int J Oncol

115

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“…Fernandes et al [15] also showed that MMP-2 expression was higher in stromal cells of invasive carcinomas than of CIN 3 and indicated that stromal cells play an important role in tumor invasion and progression, mediated by the progressive enhancement of MMP-2 expression from CIN 3 to advanced invasive tumor. Brummer et al [16] suggested that MMP-2 expression, when focally observed in high-grade intraepithelial lesions of the cervix, may indicate tumor regions with an increased risk for invasive growth.…”

Section: Discussionmentioning

confidence: 99%

Expression of MMP-2, MMP-9, MMP-14, TIMP-1, TIMP-2 in Intraepithelial and Invasive Cervical Neoplasia

Westin¹,

Rabelo-Santos²

2015

J Cytol Histol

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Aim: To evaluate the expression of MMP-2, MMP-9, MMP-14, TIMP-1 and TIMP-2 in Cervical intraepithelial neoplasia grade 3 (CIN 3) and invasive squamous cell carcinoma. Methods and Results:This study comprised three groups: Group 1: 55 cases with CIN 3; Group 2: 30 cases with CIN 3 and invasive carcinoma components; Group 3: 46 cases with invasive carcinoma. Protein expression was investigated in tumor and stromal cells by immunohistochemistry and percentages of immunostained cells were determined. The mean percentage of MMP-14 tumor cells was significantly higher in stromal cells in all groups. TIMP-2 and MMP-9 expression was significantly higher in stromal cells than in tumor cells. TIMP-1 had a significantly higher expression in stromal cells of carcinoma and tumor cells of CIN 3. Stromal cells expression of MMP-2, MMP-14, TIMP-1 was increased with the severity of cervical neoplasia. The expression of MMP-2 in stromal cells that was higher in CIN 3 component of Group 2 than in CIN 3 of Group 1. Conclusions:The increase in MMP-2 expression from CIN 3 to invasive cervical cancer reinforces their role in cervical cancer progression. From CIN 3 to invasive cervical carcinoma, TIMP-1 expression increased in stromal cells and decreased in tumor cells.

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“…For the evaluation of the immunohistochemically stained sections, the following modified semi-quantitative scheme (developed by Brummer et al 2002) was used: K, no positive staining; (C), only sporadic positive cells, !10%;C, a few cells with positive reaction, ca. 10-40%;CC, several positive cells, ca.…”

Section: Discussionmentioning

confidence: 99%

“…The changes obtained by histology were further analyzed by immunohistochemistry using the schema described previously (Brummer et al 2002). The expression of ESR1 (ERa) showed the following results: in the sections of the hormonally treated groups, many positive red-stained epithelial and stromal cell nuclei (CCC) were apparent.…”

Section: Immunohistochemistrymentioning

confidence: 99%

The hormonal induction of cervical remodeling in the common marmoset monkey (Callithrix jacchus)

Simon¹,

Einspanier

2009

Reproduction

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Controversy still exists regarding the involvement of relaxin (RLX) in cervical reorganization throughout parturition in the human, despite its well-known role in facilitating extensive extracellular matrix (ECM) remodeling in diverse organs. Therefore, the aim of the present study was to examine the influence of RLX and estrogen (E2) on the cervical tissue of the common marmoset monkey. Two experimental designs were used: 1) in vivo analysis of the intracervical diameter under locally applied RLX and 2) ovariectomized (ov) marmosets were treated systemically with either recombinant human (rh) RLX, E2 or rhRLXCE2 to examine their action on the cervix. In vivo-locally applied rhRLX induced a distinct and significant widening of the cervix (before: 4.8G1.1 mm versus after: 5.7G0.9 mm in diameter; P!0.030, MVGS.E.M.). This widening effect was most pronounced in animals without previous pregnancies. In vitro investigation of cervical tissue showed significantly increased wet weights after all three hormone treatments (E2: 0.27G0.07 g, RLX: 0.25G0.04 g, E2C RLX: 0.30G0.11 g; all P!0.05; MVGS.E.M.) versus controls (0.10G0.04 g). Furthermore, morphological changes such as loosening of the connective tissue structure and decline in collagen content, an increase in the number of eosinophils, increased the expression of matrix metalloproteinases (MMP1) and MMP2, as well as gene and protein expression of the RLX receptor RXFP1 could be detected in the cervical tissue after all hormone treatments, compared with controls. In summary, RLX has a potent widening effect on the cervix of the common marmoset monkey. Although E2 is not required for this RLX effect, a combined application of E2 and RLX induced the most prominent cervical ripening.

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MMP-1 and MMP-2 in the Cervix Uteri in Different Steps of Malignant Transformation—An Immunohistochemical Study

Cited by 62 publications

References 23 publications

Uterine cervical carcinoma: Role of matrix metalloproteinases (Review)

Uterine cervical carcinoma: Role of matrix metalloproteinases (Review)

Expression of MMP-2, MMP-9, MMP-14, TIMP-1, TIMP-2 in Intraepithelial and Invasive Cervical Neoplasia

The hormonal induction of cervical remodeling in the common marmoset monkey (Callithrix jacchus)

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