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Abstract

Hydroxypropyl-beta-cyclodextrin was analyzed by HPLC using postcolumn complexation with phenolphthalein and negative colorimetric detection, with a detection limit of 20 micrograms/ml. The pharmacokinetics of beta-cyclodextrin and of hydroxypropyl-beta-cyclodextrin were studied after intravenous administration to permanently cannulated rats. The pharmacokinetic behavior of both cyclodextrins was similar to that of inulin, showing rapid distribution over extracellular fluids. Elimination occurred through glomerular filtration. When a dose of 200 mg/kg beta-cyclodextrin was administered the elimination rate was decreased, probably as a result of nephrotoxicity of beta-cyclodextrin. Within 24 hr after administration most of the cyclodextrin dose was recovered unchanged in urine. After oral administration, only insignificant amounts of intact beta-cyclodextrin were absorbed from the gastrointestinal tract.

Preliminary Safety Evaluation of Parenterally Administered Sulfoalkyl Ether β-Cyclodextrin Derivatives

“…23 The elimination of p-CD at these levels is decreased due to probable decrease in glomerular filtration rate resulting from the nephrotoxic effects of the p-CD. It is expected that the sulfoalkyl ether derivatives studied would be eliminated in a manner similar to that of HP-P-CD and inulin, as observed by Frijlink and others.23 Interpretation of differences, if any, in the rate and extent of excretion of the sulfoalkyl ether derivatives and HP-p-CD would be speculative, however, as the error associated with urine collection and analysis of samples in these studies may be significant.…”

Quantitative analysis of natural cyclodextrins by high-performance liquid chromatography with pulsed amperometric detection: Application to cell permeation study

“…In most cases, the separation of natural CDs has been carried out by HPLC with reversed-phase columns [4,[8][9][10][12][13][14][15], while few applications with normal phase [2,25] and anion exchange [11] chromatography have been described. Furthermore, solid-phase extraction [5,8,10], ultrafiltration [9,12] and centrifugation [13,14] have been used as the pretreatment methods of biological samples containing natural CDs.…”

“…Furthermore, solid-phase extraction [5,8,10], ultrafiltration [9,12] and centrifugation [13,14] have been used as the pretreatment methods of biological samples containing natural CDs. In the present study, the reversed-phase columns (Zorbax SB-Aq and Zorbax SB-Phenyl) provided good chromatographic performance for the aqueous ␣-CD, ␤-CD and ␥-CD samples.…”

“…In contrast to the direct detection methods, the use of photometric or fluorescence-based detection of CDs requires a complexation reaction with a specific UV-vis-absorptive of fluorescent reagent prior to the detection [13][14][15][16][17][18][19][20][21][22][23]. In general, with these indirect methods, sensitivities up to micromolar levels have been obtained.…”

Therapeutic potential of cyclodextrins in the treatment of Niemann–Pick type C disease

“…2HPBCD is cleared by glomerular filtration in the kidneys and excreted unchanged into urine [95]. When 2HPBCD was administered subcutaneously to adult 49-day-old mice with fully matured renal function at a dose of 4000 mg/kg, the time it took for the clearance of half the administered dose of 2HPBCD from the body ( t 1/2 ) was approximately 1.6 h [96].…”