MLST reveals potentially high-risk international clones of Enterobacter cloacae*

Abstract

Together with the information available in the MLST database, our results suggest that, like Escherichia coli and Klebsiella pneumoniae, E. cloacae harbours clonal lineages of increased epidemic potential that may be associated with resistance spread.

Clonal distribution of multidrug-resistant Enterobacter cloacae

“…1). This result is consistent with results from Izdebski et al (2015), who reported very recently a clonal diversity of 97.5% among expanded-spectrum cephalosporin-resistant E. cloacae isolates from Europe and Israel (Izdebski et al, 2015). The discriminatory ability of the different loci, measured as the number of alleles obtained for each gene, varied from 17 (for rpoB and rplB) to 21 (for dnaA) ( Table 1).…”

“…This result differs from that obtained by Miyoshi-Akiyama et al (2013), reporting the leuS and pyrG alleles as the most polymorphic ones. The result of the nucleotide diversity analysis within the sample, performed on alleles of each MLST gene, demonstrated significant diversity in accordance with the data from Miyoshi-Akiyama et al (2013) and Izdebski et al (2015).…”

“…The phylogenetic analysis of Izdebski et al (2015) performed on 195 E. cloacae resistant to expanded spectrum cephalosporins and collected across Europe and Israel distinguished 80 STs and contained 12 clusters of very related STs. Comparing with those data, we were able to redefine our lineages accordingly, aiming to make both studies corremating as much as possible.…”

“…Comparing with those data, we were able to redefine our lineages accordingly, aiming to make both studies corremating as much as possible. The phylogenetic analysis was, therefore, completed by including the 12 STs representing the 12 different clusters as defined by Izdebski et al (2015). The first 4 lineages could be well correlated with those (A to D) from Izdebski et al (2015), but some STs that had not been previously identified could not be related to any of the previously described clusters.…”

Occurrence of carbapenemase-producing Klebsiella pneumoniae and Escherichia coli in the European survey of carbapenemase-producing Enterobacteriaceae (EuSCAPE): a prospective, multinational study

“…These values will underestimate total CPE incidence, because carba penemases also occur in other Enterobacteriaceae, albeit less frequently than in Klebsiella spp. 17 Moreover, the absence of denominator data from eight countries cautions against our ranking of prevalence. Proportions of carbapenemase-positive bacteria considered in this study varied between countries and between the two species under investigation (table 2, table 3).…”