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Magot, Michel; Ollivier, Bernard; Patel, Bharat K. C.

doi:10.1023/a:1002434330514

Cited by 465 publications

(135 citation statements)

References 71 publications

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“…These thermophiles can survive under lower-temperature conditions, as shown for other cold environments (49)(50)(51). This has led to the suggestion that thermophiles in oil fields originated from the low concentrations present in seawater injected to promote oil recovery (1,(52)(53)(54) and, conversely, that low numbers of thermophiles in cold marine sediments may have originated from seeps from hot oil fields, where they may be part of the indigenous microbial community (49,53,(55)(56)(57).…”

Section: Discussionmentioning

confidence: 97%

Implications of Limited Thermophilicity of Nitrite Reduction for Control of Sulfide Production in Oil Reservoirs

Fida

Chen

Okpala

et al. 2016

Appl Environ Microbiol

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Nitrate reduction to nitrite in oil fields appears to be more thermophilic than the subsequent reduction of nitrite. Concentrated microbial consortia from oil fields reduced both nitrate and nitrite at 40 and 45°C but only nitrate at and above 50°C. The abundance of the nirS gene correlated with mesophilic nitrite reduction activity. Thauera and Pseudomonas were the dominant mesophilic nitrate-reducing bacteria (mNRB), whereas Petrobacter and Geobacillus were the dominant thermophilic NRB (tNRB) in these consortia. The mNRB Thauera sp. strain TK001, isolated in this study, reduced nitrate and nitrite at 40 and 45°C but not at 50°C, whereas the tNRB Petrobacter sp. strain TK002 and Geobacillus sp. strain TK003 reduced nitrate to nitrite but did not reduce nitrite further from 50 to 70°C. Testing of 12 deposited pure cultures of tNRB with 4 electron donors indicated reduction of nitrate in 40 of 48 and reduction of nitrite in only 9 of 48 incubations. Nitrate is injected into high-temperature oil fields to prevent sulfide formation (souring) by sulfate-reducing bacteria (SRB), which are strongly inhibited by nitrite. Injection of cold seawater to produce oil creates mesothermic zones. Our results suggest that preventing the temperature of these zones from dropping below 50°C will limit the reduction of nitrite, allowing more effective souring control. IMPORTANCENitrite can accumulate at temperatures of 50 to 70°C, because nitrate reduction extends to higher temperatures than the subsequent reduction of nitrite. This is important for understanding the fundamentals of thermophilicity and for the control of souring in oil fields catalyzed by SRB, which are strongly inhibited by nitrite.

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Section: Discussionmentioning

confidence: 97%

Implications of Limited Thermophilicity of Nitrite Reduction for Control of Sulfide Production in Oil Reservoirs

Fida

Chen

Okpala

et al. 2016

Appl Environ Microbiol

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“…Microorganisms populating these environments are generally considered (poly) extremophiles, attributing several unusual and interesting traits [3]. However, studies of these microbes are limited by difficulties in accessing relevant oil reservoirs, as well as technical challenges in representative oil reservoir sampling [4] [5]. Due to their nature, oil reservoir microorganisms with their special adaptations are potential high value targets for the discovery of useful biocatalysts for industrial processes [6]- [8].…”

Section: Introductionmentioning

confidence: 99%

Discovery and Characterization of a Thermostable Esterase from an Oil Reservoir Metagenome

Lewin¹,

Strand²,

Haugen³

et al. 2016

AER

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With the aim of identifying novel thermostable esterases, comprehensive sequence databases and cloned fosmid libraries of metagenomes derived from an offshore oil reservoir on the Norwegian Continental Shelf were screened for enzyme candidates using both sequence-and function-based screening. From several candidates identified in both approaches, one enzyme discovered by the functional approach was verified as a novel esterase and subjected to a deeper characterization. The enzyme was successfully over-produced in Escherichia coli and was shown to be thermostable up to 90˚C, with the highest esterase activity on short-chain ester substrates and with tolerance to solvents and metal ions. The fact that the thermostable enzyme was solely found by functional screening of the oil reservoir metagenomes illustrates the importance of this approach as a complement to purely sequence-based screening, in which the enzyme candidate was not detected. In addition, this example indicates the large potential of deep-sub-surface oil reservoir metagenomes as a source of novel, thermostable enzymes of potential relevance for industrial applications.

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“…4) SRB are anaerobic microorganisms which carry out dissimilatory reduction of sulfate to obtain energy, resulting in the release of a great quantity of H 2 S gas. Considerable work has centered on the role of SRB in the corrosion process.…”

Section: Introductionmentioning

confidence: 99%

Biocorrosion of Low Alloy Steel by Desulfotomaculum sp. and Effect of Biocides on Corrosion Control

2007

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Untitled

Cited by 465 publications

References 71 publications

Implications of Limited Thermophilicity of Nitrite Reduction for Control of Sulfide Production in Oil Reservoirs

Implications of Limited Thermophilicity of Nitrite Reduction for Control of Sulfide Production in Oil Reservoirs

Discovery and Characterization of a Thermostable Esterase from an Oil Reservoir Metagenome

Biocorrosion of Low Alloy Steel by Desulfotomaculum sp. and Effect of Biocides on Corrosion Control

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