MKI67 (marker of proliferation Ki-67)

Ntzeros, Konstantinos; Philip, Subha; Mazis, Dimitrios; Kritikos, N; Rozis, M; Anesidis, E; Antoniou, Chrisoula

doi:10.4267/2042/56295

Cited by 4 publications

(4 citation statements)

References 82 publications

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“…BIRC5 plays a critical role in apoptosis, cell division and proliferation 58 . MKI67 triggered in differentiated cells after proliferative stimulation and disappeared after proliferating cell differentiation, which was used as a tumour proliferation marker 59 . Besides the above genes involved in the cell cycle‐related pathways, we found several genes in the inflammatory pathway were closely related to inhibited IgE production.…”

Section: Discussionmentioning

confidence: 86%

Anti‐IgE effect of small‐molecule‐compound arctigenin on food allergy in association with a distinct transcriptome profile

Cao

Liu

Lin

et al. 2021

Clin Experimental Allergy

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Background: Excessive production of IgE plays a major role in the pathology of food allergy. In an attempt to identify anti-IgE natural products, Arctium Lappa was one of the most effective herbs among approximately 300 screened medicinal herbs. However, little is known about its anti-IgE compounds.Objective: To identify compounds from Arctium Lappa for targeted therapy on IgE production and explore their underlying mechanisms. Methods:Liquid-liquid extraction and column chromatographic methods were used to purify the compounds. IgE inhibitory effects were determined on IgE-producing human myeloma U266 cells, peanut-allergic murine model and PBMCs from foodallergic patients. Genes involved in IgE inhibition in PBMCs were studied by RNA sequencing. Results:The main compounds isolated were identified as arctiin and arctigenin. Both compounds significantly inhibited IgE production in U266 cells, with arctigenin the most potent (IC50=5.09μg/mL). Arctigenin (at a dose of 13 mg/kg) markedly reduced peanut-specific IgE levels, blocked hypothermia and histamine release in a | 251 CAO et Al. | INTRODUC TI ONFood allergy (FA) is a significant, worsening global public health issue. Over 2 decades, paediatric and adult incidence has increased dramatically in developed and some developing countries. 1-3 Foodinduced reactions account for 81% of paediatric anaphylaxis cases. 4 Despite worldwide prevalence, FA lacks sufficient therapies. Food avoidance and emergency injectable epinephrine remain the major standard of care, although the first FDA treatment, Palforzia ® , an oral immunotherapy for peanut allergy, was approved in January 2020. Food restriction, social isolation, fear and anxiety, particularly among teens, 5-7 present complex familial and social challenges. [8][9][10][11] peanut-allergic mouse model. Arctigenin also significantly reduced IgE production and Th2 cytokines (IL-5, IL-13) by PBMCs. We found 479 differentially expressed genes in PBMCs with arctigenin treatment (p < .001 and fold-change ≥1.5), involving 24 gene ontology terms (p < .001, FDR <0.05); cell division was the most significant. Eleven genes including UBE2C and BCL6 were validated by qPCR. Conclusion:Arctigenin markedly inhibited IgE production in U266 cells, peanutallergic murine model and PBMCs from allergic patients by down-regulating cell division, cell cycle-related genes and up-regulating anti-inflammatory factors.

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Section: Discussionmentioning

confidence: 86%

Anti‐IgE effect of small‐molecule‐compound arctigenin on food allergy in association with a distinct transcriptome profile

Cao

Liu

Lin

et al. 2021

Clin Experimental Allergy

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“…dTMP is a precursor of thymidine, an essential building block for DNA replication and repair. Ki‐67 ( MKI67 ) is a protein that is commonly used as a marker to measure the proliferation rate of cells and determine the grade of certain tumours, detected through IHC staining 72 …”

Section: Discussionmentioning

confidence: 99%

“…Ki-67 (MKI67) is a protein that is commonly used as a marker to measure the proliferation rate of cells and determine the grade of certain tumours, detected through IHC staining. 72 The top five differential genes in LumB tumours, ranked by significance, are MIS18A, WIF1, CHEK2, EMCN and CDK4. MIS18A is involved in chromosome segregation during cell division by maintaining the centromeres, 73 whereas WIF1 regulates the Wnt signalling pathway, 74 which is essential for various cellular processes.…”

Section: Crosstalk Between Metabolism and Genesmentioning

confidence: 99%

Image‐guided metabolomics and transcriptomics reveal tumour heterogeneity in luminal A and B human breast cancer beyond glucose tracer uptake

Yang,

Deng,

Preibsch

et al. 2024

Clinical & Translational Med

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BackgroundBreast cancer is a metabolically heterogeneous disease, and although the concept of heterogeneous cancer metabolism is known, its precise role in human breast cancer is yet to be fully elucidated.MethodsWe investigated in an explorative approach a cohort of 42 primary mamma carcinoma patients with positron emission tomography/magnetic resonance imaging (PET/MR) prior to surgery, followed by histopathology and molecular diagnosis. From a subset of patients, which showed high metabolic heterogeneity based on tracer uptake and pathology classification, tumour centre and periphery specimen tissue samples were further investigated by a targeted breast cancer gene expression panel and quantitative metabolomics by nuclear magnetic resonance (NMR) spectroscopy. All data were analysed in a combinatory approach.Results[18F]FDG (2‐deoxy‐2‐[fluorine‐18]fluoro‐d‐glucose) tracer uptake confirmed dominance of glucose metabolism in the breast tumour centre, with lower levels in the periphery. Additionally, we observed differences in lipid and proliferation related genes between luminal A and B subtypes in the centre and periphery. Tumour periphery showed elevated acetate levels and enrichment in lipid metabolic pathways genes especially in luminal B. Furthermore, serine was increased in the periphery and higher expression of thymidylate synthase (TYMS) indicated one‐carbon metabolism increased in tumour periphery. The overall metabolic activity based on [18F]FDG uptake of luminal B subtype was higher than that of luminal A and the difference between the periphery and centre increased with tumour grade.ConclusionOur analysis indicates variations in metabolism among different breast cancer subtypes and sampling locations which details the heterogeneity of the breast tumours. Correlation analysis of [18F]FDG tracer uptake, transcriptome and tumour metabolites like acetate and serine facilitate the search for new candidates for metabolic tracers and permit distinguishing luminal A and B. This knowledge may help to differentiate subtypes preclinically or to provide patients guide for neoadjuvant therapy and optimised surgical protocols based on individual tumour metabolism.

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“…Ki-67 has been recorded to be an excellent marker for estimating the growth fraction in both normal and malignant human tissue, and it is currently utilized as the golden standard for assessing cell proliferation instead of PCNA since it is less impacted by internal and external factors. Antigen Ki-67 also commonly known as Ki-67 or MKI67 (Marker of proliferation Ki-67), is a protein in humans that is encoded by the MKI67 gene [45,46,47] . Its function as a biological marker of mitotic activity is aided by its nuclear expression throughout a specific period of the cell cycle.…”

Section: Loss Of Basilar Polarity Observed In Subgroupmentioning

confidence: 99%

The Effect of Sofosbuvir Administration on Albino Rats’ Buccal Mucosa (Histological and immunochemical study)

Elbatrick

Halawa

Taha

et al. 2022

Egyptian Dental Journal

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Introduction: Hepatitis C virus (HCV) infection is a principal cause of mortality globally. About 170 million people were infected with HCV worldwide. Egypt's Ministry of Health and Population has released a nationwide strategy to address this pandemic. Sofosbuvir is a new combination of direct-acting antiviral drugs that doesn't require addition of interferon and is an effective alternative to interferon. The systemic effects of Sofosbuvir are being investigated nowadays; however, there aren't enough studies on its impact on oral health yet. Aim of the study:To investigate the effect of Sofosbuvir on buccal mucosa in Albino rats.Materials and Methods: Twenty-eight Adult male albino rats were randomly divided into two main groups, fourteen rats each. Each group was furtherly subdivided into two subgroups A & B according to the time of sacrifice which was 45 and 60 days respectively. Control group (GroupI) was given distilled water daily by gastric tube, and experimental group (GroupII) was given Sofosbuvir (40 mg/kg/day) dissolved in distilled water by gastric tube. Specimens were examined histologically and immunohistochemically.Results: Group II showed vacuolization in the epithelium and signs of degeneration in both epithelium and connective tissue. Immunohistochemical results of GroupI showed strong positive nuclear immunoreactivity for Ki-67 in the epithelial cells and lamina propria, however GroupII showed weak nuclear immunoreactivity for Ki-67. Statistical results showed significant decrease in Ki-67 positive area percentage of SubgroupIIB when compared to SubgroupIIA. Conclusion:Sofosbuvir administration resulted in degenerative changes in buccal mucosa and affected its proliferation, which was time dependent.

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MKI67 (marker of proliferation Ki-67)

Abstract: Review on MKI67, with data on DNA/RNA, on the protein encoded and where the gene is implicated.

Cited by 4 publications

References 82 publications

Anti‐IgE effect of small‐molecule‐compound arctigenin on food allergy in association with a distinct transcriptome profile

Anti‐IgE effect of small‐molecule‐compound arctigenin on food allergy in association with a distinct transcriptome profile

Image‐guided metabolomics and transcriptomics reveal tumour heterogeneity in luminal A and B human breast cancer beyond glucose tracer uptake

The Effect of Sofosbuvir Administration on Albino Rats’ Buccal Mucosa (Histological and immunochemical study)

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