Mixture of endophytic Agrobacterium and Sinorhizobium meliloti strains could induce nonspecific nodulation on some woody legumes

Liu, Jie; Wang, En Tao; Ren, Da Wei; Chen, Wen Xin

doi:10.1007/s00203-010-0543-2

Cited by 38 publications

(21 citation statements)

References 19 publications

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“…CT5. These species have already been reported as endophytes of many plants (Goryluk et al 2009;Khan and Doty 2009;Ma et al 2009;Liu et al 2010;Rashid et al 2012). Endophytes like Bacillus subtilis, Bacillus sp., and Pseudomonas putida are known for their ability to produce indole acetic acid (IAA) (Khan and Doty 2009;He et al 2010).…”

Section: Discussionmentioning

confidence: 95%

Isolation and characterization of bacterial endophytes from the roots of Cassia tora L

Kumar

Pandey

et al. 2014

Ann Microbiol

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In the present investigation, five endophytic bacterial strains isolated from the roots of Cassia tora L. were identified as Bacillus subtilis, Agrobacterium tumefaciens, Bacillus sp., Pseudomonas putida, and Pseudomonas sp. based on biochemical characteristics as well as 16S rRNA gene sequencing. Isolates were screened for plant growth promoting traits, antibacterial activity, antifungal activity, antibiotic sensitivity, and salinity tolerance. The majority of the endophytic strains produced phytohormone indole acetic acid (IAA), ammonia, and also solubilized phosphate. Siderophore and HCN production were observed in A. tumefaciens, P. putida, and Pseudomonas sp. The antibiotic sensitivity profile indicated that the isolates were resistant to chloramphenicol, while highly susceptible to neomycin and streptomycin. Bacterial endophytes gave a definite stamp on their antibacterial activity against Escherichia coli and Klebsiella pneumoniae and antifungal activity against Pythium ultimum, Aureobasidium pullulans, and Alternaria alternata. Pseudomonas sp. showed maximum salt tolerance (6 % NaCl) contrary to A. tumefaciens that was least tolerant.

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Section: Discussionmentioning

confidence: 95%

Isolation and characterization of bacterial endophytes from the roots of Cassia tora L

Kumar

Pandey

et al. 2014

Ann Microbiol

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“…Recently, many Agrobacterium strains have been isolated from root nodules of legume plants and though most were non-symbiotic belonging to Agrobacterium tumefaciens, some may represent novel biovars (19). Moreover, Velazquez et al (39) reported for the first time the occurrence of two strains (163C and ATCC 11325 T ) belonging to R. rhizogenes able to nodulate Phaseolus vulgaris.…”

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confidence: 99%

Stress Tolerance and Symbiotic and Phylogenic Features of Root Nodule Bacteria Associated with Medicago Species in Different Bioclimatic Regions of Tunisia

et al. 2011

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Thirty two rhizobial isolates were obtained from different bioclimatic regions of Tunisia using as trap plants, Medicago sativa, Medicago ciliaris, Medicago polymorpha and Medicago minima. To study their diversity and characterize them in relation to Mediterranean conditions, abiotic stress resistance, symbiotic properties and genetic diversity in terms of 16S rRNA and nodA sequences were assessed. Five isolates from M. sativa, three from M. ciliaris and three from M. minima could grow at 45°C. Only two isolates from M. sativa grew at 4% NaCl. The most stress tolerant isolates were obtained from arid soils. A phylogenetic analysis of 16S rRNA genes revealed 29 isolates to be closely related to Ensifer including one (Pl.3-9) that showed a 16S rRNA sequence similar to that of Ensifer meliloti and nodA sequence similar to that of Ensifer medicae. However, three isolates were categorized into Agrobacterium containing the nodA of Ensifer. Furthermore, these isolates developed nodules on original hosts. The results for the four isolates suggest horizontal gene transfer between the species.

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“…Belonging to this bacterial phylum, Bradyrhizobium, Rhizobium and Sinorhizobium lineages were detected as main endophytes (Tables 2 and 4). Previously, existence of endophytic Rhizobium radiobacter (=Agrobacterium tumefaciens) strains in nodules or roots of many legumes and their interaction with symbiotic bacteria have been studied (Liu et al 2010;Mrabet et al 2006), but the existence of symbiotic bacteria as endophytes in the same host plant has not been well studied. It was interesting that the inoculated B. liaoningense strain occupied 75% and 80% of the nodules at stage R2 in the treatments of MSR and ISR, respectively, but its abundance as root endophyte was less than that of Sinorhizobium.…”

Section: Discussionmentioning

confidence: 99%

Effects of rhizobial inoculation, cropping systems and growth stages on endophytic bacterial community of soybean roots

Zhang

Wang

et al. 2011

Plant Soil

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The effects of rhizobial inoculation, soybean-maize intercropping and plant growth stages on the diversity of soybean root endophytic bacteria were evaluated. PCR-based terminal restriction fragment length polymorphism (T-RFLP) of 16S rRNA genes and analyses of cloned partial 16S rRNA gene libraries were used to study the endophytic bacterial communities. The root endophytic bacterial populations during the whole growing season was significantly affected by the three factors analyzed, with plant growth and rhizobial inoculation showing the highest and lowest impact. The alphaproteobacteria and betaproteobacteria were the most predominant endophytes of soybean roots. Some undefined bacterial lineages were also found, indicating that the root endophytic populations might constitute an important source for the isolation of novel bacteria. Bradyrhizobium liaoningense (used as inoculant) occupied most of the soybean nodules formed and Sinorhizobium americanum-related bacteria were detected as the main root endophytes. Results also suggest that bacteria might be transferred from nodules to roots (as endophytic bacteria) when the seeds become mature, which might be a possible process in the life cycle of rhizobia. Rhizobial inoculation and intercropping practices could increase the diversity of the soybean root endophytes, which might have a positive influence on the growth of soybean plants.

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Mixture of endophytic Agrobacterium and Sinorhizobium meliloti strains could induce nonspecific nodulation on some woody legumes

Cited by 38 publications

References 19 publications

Isolation and characterization of bacterial endophytes from the roots of Cassia tora L

Isolation and characterization of bacterial endophytes from the roots of Cassia tora L

Stress Tolerance and Symbiotic and Phylogenic Features of Root Nodule Bacteria Associated with Medicago Species in Different Bioclimatic Regions of Tunisia

Effects of rhizobial inoculation, cropping systems and growth stages on endophytic bacterial community of soybean roots

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