Mixotrophic culture of bait microalgae for biomass and nutrients accumulation and their synergistic carbon metabolism

Bo, Yahui; Chu, Ruirui; Sun, Danni; Deng, Xiangyuan; Zhou, Chengxu; Yan, Xiaojun; Ruan, Roger; Cheng, Pengfei

doi:10.1016/j.biortech.2022.128301

Cited by 16 publications

(4 citation statements)

References 44 publications

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“…Various previous test results indicate that glucose [18], glycerol [25] and sucrose [5] were suitable carbon sources for microalgae cultivation. In order to choose the most appropriate combinations of different carbon and nitrogen source ratios for cell growth and FAMEs accumulation of Chlorella sp.…”

Section: Carbon and Nitrogen Sources Concentration Effectmentioning

confidence: 93%

Enhancing Lipid Production of Chlorella sp. by Mixotrophic Cultivation Optimization

Lay

Abdul

et al. 2023

Processes

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Mixotrophic microalgal cultivation can utilize CO2 and organic carbon sources. This study optimized the cultivation nitrogen source (peptone, urea, yeast extract, NH4Cl, (NH4)2SO4, NH4NO3, NaNO3 and KNO3), carbon combination (glucose, glycerol, sucrose), (NH4)2SO4 nitrogen source and pH (6–11) in a local microalgae species with three characteristics (high pH-resistant, high growth rate and high lipid content). Chlorella sp. G3H3-1-2 biomass production and lipid accumulation were estimated using the fatty acid methyl esters (FAMEs) concentration. The Chlorella sp. G3H3-1-2 FAME content was strongly influenced by the carbon, nitrogen sources and pH variations. The pH ranged from 6.0 to 8.0, which produced the highest specific growth rate of 1.22 day−1 for Chlorella sp. G3H3-1-2 while using glucose as the single carbon source. However, the highest total FAMEs content of 59% in the Chlorella sp. G3H3-1-2 biomass of 1.75 g/L was obtained while using the combination of 1 g-glucose/L as the carbon source and 0.2 g-(NH4)2SO4/L as the nitrogen source at the high pH value of 10.

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Section: Carbon and Nitrogen Sources Concentration Effectmentioning

confidence: 93%

Enhancing Lipid Production of Chlorella sp. by Mixotrophic Cultivation Optimization

Lay

Abdul

et al. 2023

Processes

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“…Few attempts have been made to obtain high cell density with high cellular photosynthetic components. One potential route is mixotrophic culture of microalgae, but the contribution of this process to improving biomass production is limited [ 51 ]. Ogbonna et al [ 52 ] first reported increase in chlorophyll and protein contents by transferring a highly concentrated microalgal culture from a 2.5 L fermenter to a photobioreactor, which confirmed the possibility that the nutrient composition of microalgae in heterotrophic production can be improved by supplementing light.…”

Section: Biomass Production Systems: From Phototrophic To High Cell-d...mentioning

confidence: 99%

Biotechnologies for bulk production of microalgal biomass: from mass cultivation to dried biomass acquisition

Qin,

Wang,

Gao

et al. 2023

Biotechnol Biofuels

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Microalgal biomass represents a sustainable bioresource for various applications, such as food, nutraceuticals, pharmaceuticals, feed, and other bio-based products. For decades, its mass production has attracted widespread attention and interest. The process of microalgal biomass production involves several techniques, mainly cultivation, harvesting, drying, and pollution control. These techniques are often designed and optimized to meet optimal growth conditions for microalgae and to produce high-quality biomass at acceptable cost. Importantly, mass production techniques are important for producing a commercial product in sufficient amounts. However, it should not be overlooked that microalgal biotechnology still faces challenges, in particular the high cost of production, the lack of knowledge about biological contaminants and the challenge of loss of active ingredients during biomass production. These issues involve the research and development of low-cost, standardized, industrial-scale production equipment and the optimization of production processes, as well as the urgent need to increase the research on biological contaminants and microalgal active ingredients. This review systematically examines the global development of microalgal biotechnology for biomass production, with emphasis on the techniques of cultivation, harvesting, drying and control of biological contaminants, and discusses the challenges and strategies to further improve quality and reduce costs. Moreover, the current status of biomass production of some biotechnologically important species has been summarized, and the importance of improving microalgae-related standards for their commercial applications is noted.

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“…Several studies have examined the growth rates and total lipid contents of various microalgal species supported by glycerol [ 17 , 22 , 23 , 24 ] ( Table 1 ). These studies’ findings have shown improvements in mass culture productivity compared to phototrophic cultures.…”

Section: Introductionmentioning

confidence: 99%

A Response Surface Methodology Study for Chlorella vulgaris Mixotrophic Culture Optimization

Rincon,

Beyenal,

Romero

2024

Microorganisms

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Glycerol is a carbon source that produces good biomass under mixotrophic conditions. Enhancing the composition of culture media in algae biomass production improves growth rates, biomass yield, nutrient utilization efficiency, and overall cost-effectiveness. Among the key nutrients in the medium, nitrogen plays a pivotal role. Urea can be effectively used as a nitrogen source and is considered a low-cost form of nitrogen compared to other sources. Urea metabolism releases some CO2 in photosynthesis, and magnesium plays a major role in urea uptake. Magnesium is another key nutrient that is key in photosynthesis and other metabolic reactions. To maximize glycerol consumption in the mixotrophic system and to obtain high biomass and lipid productions, the variations in MgSO4·7H2O and urea concentrations were evaluated in the growth medium of the microalgae. A response surface methodology (RSM) using a central composite design (CCD) was designed to maximize glycerol consumption at the initial cellular growth rates (up to four days). The magnesium and urea supply varied from 0.3 to 1.7 g L−1. Response surface methodology was utilized to analyze the results, and the highest glycerol consumption rate, 770.2 mg L−1 d−1, was observed when C. vulgaris was grown at 1.7 g L−1 urea, 1.0 g L−1 MgSO4·7H2O. Using the optimal urea and magnesium concentrations with acetate, glucose, and glycerol as carbon sources, the same lipid content (10% average) was achieved on day 4 of mixotrophic C. vulgaris culture. Overall, the results show that mixotrophic growth of C. vulgaris using urea with an optimum magnesium concentration yields large amounts of fatty acids and that the carbon source greatly influences the profile of the fatty acids.

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Mixotrophic culture of bait microalgae for biomass and nutrients accumulation and their synergistic carbon metabolism

Cited by 16 publications

References 44 publications

Enhancing Lipid Production of Chlorella sp. by Mixotrophic Cultivation Optimization

Enhancing Lipid Production of Chlorella sp. by Mixotrophic Cultivation Optimization

Biotechnologies for bulk production of microalgal biomass: from mass cultivation to dried biomass acquisition

A Response Surface Methodology Study for Chlorella vulgaris Mixotrophic Culture Optimization

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