Mixotrophic cultivation promotes growth, lipid productivity, and PUFA production of a thermophilic Chlorophyta strain related to the genus Graesiella

Zili, Fatma; Bouzidi, Nahla; Ammar, Jihene; Zakhama, Wiem; Ghoul, Mohamed; Sayadi, Sami; Ouada, Hatem Ben

doi:10.1007/s10811-016-0941-1

Cited by 24 publications

(14 citation statements)

References 63 publications

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“…Biomass and biochemical composition value 69 mgL -1 day -1 at 75%WW treatment. These results are consistent with those obtained for some other microalgae [32,33]. Lipid accumulation by Asterarcys quadricellulare cultivated on different concentrations of municipal wastewater and BBM (control) are presented in Table 2 and Fig.…”

Section: Growth Of Asterarcys Quadricellulare In Municipal Wastewatersupporting

confidence: 91%

Coupling of Nutrient Removal and Biodiesel Production By Asterarcys quadricellulare Microalga Grown in Municipal Wastewater

Morsi¹,

Eladel²,

Maher³

2021

Preprint

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The present study focused on the feasibility of using municipal wastewater (WW) as culture medium for cultivation of microalgae. The study aimed to assess the efficiency of microalgae in nutrients removing capacity from wastewater and its biomass and lipid productivity for using as biodiesel feedstock. Based on that, the green microalga Asterarcys quadricellulare was isolated and grown for 24 days in Bold’s Basal Medium as a control and at different concentration of secondary treated municipal wastewater (WW) diluted with distilled water (25%, 50%, 75% and 100%WW). Results of 75%WW treatment recorded 96.6%, 98.4%, and 89.9% removal efficiency for, nitrate, ammonia and total phosphorus, respectively. Also, it revealed high biomass productivity and biomass content, where it recorded 69.0 mgL-1 day-1, and 1.44 g/L, respectively. Likewise, high lipid productivity 17.2 mg L−1 day−1 and 360.6 mg/L lipid content. Consequently, Asterarcys quadricellulare fatty acids profile estimation revealed an increase in Oleic, Palmitic and Linoleic acids levels. Most properties of biodiesel derived from the studied microalga meet with values established by the ASTM D6751 and EN 14214 biodiesel standards. According to this analysis, A. quadricellulare microalga could be used for wastewater bioremediation and biomass production with a suitable content of lipids proper as biodiesel feedstock. The predictive biodiesel properties pointed that it has a good quality compared with international standards.

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Section: Growth Of Asterarcys Quadricellulare In Municipal Wastewatersupporting

confidence: 91%

Coupling of Nutrient Removal and Biodiesel Production By Asterarcys quadricellulare Microalga Grown in Municipal Wastewater

Morsi¹,

Eladel²,

Maher³

2021

Preprint

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“…The highest biomass productivity was registered at day 2 of culture and reached 0.50 ± 0.02 g dw L −1 day −1 . These values are greatly higher than that obtained in optimized Graesiella laboratory culture conditions observed by Zili et al (2017); 0.40 ± 0.01 g dw L −1 of maximal biomass density and 0.17 ± 0.03 g dw L −1 d −1 as maximal biomass productivity.…”

Section: Biomass and Eps Productioncontrasting

confidence: 63%

“…In the case where the maximum productivity of biomass and EPSs is in the opposite production phases, the use of the two-stage cultivation strategy is the solution to jointly and continuously ensure a maximized production of the algal biomass and the EPSs. This strategy has been commonly used in the production of several intracellular secondary metabolites such as lipids and carotenoids (Hosseini and Shariati 2006;Zili et al 2017) but rarely for EPSs.…”

Section: Biomass and Eps Productionmentioning

confidence: 99%

Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp.

Gongi

Cordeiro

Pinchetti³

et al. 2020

J Appl Phycol

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The present study developed a two-step strategy to enhance the production of extracellular polymeric substances (EPSs) by a thermotolerant chlorophyte, Graesiella sp., in view to their industrial valorisation. In the first step, Graesiella sp. was grown in outdoor conditions in pilot-scale photobioreactors of 100 L culture volumes. In the second step, the biomass collected in the exponential growth phase was submitted to heat stress (50°C). A joint production of biomass reaching 0.50 g dw L −1 day −1 and of EPS production reaching 1.30 g dw L −1 in 2 days was obtained. EPSs mainly contained polysaccharides (80%) and proteins (14%). FTIR and 1 HNMR revealed the presence of primary amine and sulfated groups. The EPSs contained antioxidant enzymes (SOD, CAT, and APX) maintained in an active state by the microenvironment offered by the EPSs. The EPSs were found to have a potent antioxidant activity via directly scavenging free radicals when compared to L-ascorbic acid.

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“…2b, there is a strong correlation linking the kinetics of cellular growth and EPS production, with a maximum daily productivity of up to 0.5 g L −1 day −1 for both biomass and EPSs. These values are higher than those obtained in optimized Graesiella laboratory cultures conditions, which were 0.17 g L −1 day −1 for biomass (Zili et al 2017) and 0.1 g L −1 day −1 for EPSs' maximum daily production (Mezhoud et al 2014). This phenomenon is not usually observed (Delattre et al 2016), which could be justified by the fact that the Graesiella sp.…”

Section: Microalgae Growth Kinetic and Eps Productioncontrasting

confidence: 60%

Production of exopolymer substances from the thermophilic chlorophyte Graesiella: industrial and ecological applications

et al. 2020

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Microalgal extracellular polymeric substances (EPSs) are heteropolysaccharides that have characteristics suitable for industrial and biotechnological applications. Notably, they have strong anionic nature and high hydrophobicity. Nevertheless, systematic studies to demonstrate the viability of the production of EPSs on an industrial scale are still crucial. In this research, the chlorophyte Graesiella was grown on a raceway pond to view its EPS valorization. The biomass production achieved a maximum of 1.98 g L −1 and an EPS production of 1.6 g L −1 after six production days. The Graesiella EPSs with a molecular weight above 100 kDa are sulfated exopolymers containing mainly polysaccharide (70%) and protein (16%). The EPSs produced more stable emulsions with hydrocarbons and oils than Tween-20. The emulsification indices with n-hexane (88%) and maize oil (28%) indicate the EPSs' strong emulsion-stabilizing capacity. The EPSs showed a peak flocculating percentage of 95% to kaolin suspension, with better flocculation performance than Al 2 (SO 4 ) 3 and alginate. Moreover, Graesiella EPSs had a significant effect on antimicrobial activity, significantly inhibiting fungal growth (71% for Botytis cinerea and 87% for Fusarium oxysporum), spore germination (100% of inhibition at a concentration of 1.8 g L −1 ), and mycelium growth (68% of inhibition). Also, Graesiella EPSs acted as a bactericide against Vibrio anguilaruim and Listonella anguilaruim (100% inhibition). EPSs were also found to have potent antioxidant activity compared with L-ascorbic acid. The obtained results open new perspectives to the further exploration of Graesiella sp. as a potential EPS producer, making it a promising candidate for numerous industrial applications.

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Mixotrophic cultivation promotes growth, lipid productivity, and PUFA production of a thermophilic Chlorophyta strain related to the genus Graesiella

Cited by 24 publications

References 63 publications

Coupling of Nutrient Removal and Biodiesel Production By Asterarcys quadricellulare Microalga Grown in Municipal Wastewater

Coupling of Nutrient Removal and Biodiesel Production By Asterarcys quadricellulare Microalga Grown in Municipal Wastewater

Extracellular polymeric substances with high radical scavenging ability produced in outdoor cultivation of the thermotolerant chlorophyte Graesiella sp.

Production of exopolymer substances from the thermophilic chlorophyte Graesiella: industrial and ecological applications

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