Mixotrophic cultivation, a preferable microalgae cultivation mode for biomass/bioenergy production, and bioremediation, advances and prospect

Zhan, Jiao; Rong, Junfeng; Wang, Qiang

doi:10.1016/j.ijhydene.2016.12.021

Cited by 213 publications

(80 citation statements)

References 136 publications

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“…It is generally known that many microalgae species possess an inducible hexose/H + active symport system to uptake and catabolize organic substrates as C and energy sources . Thus, addition of glucose to the media could accelerate cell growth rate and increase algal biomass relative to the photoautotrophic cultures …”

Section: Resultscontrasting

confidence: 61%

Glucose addition‐induced changes in the growth and chemical compositions of a freshwater microalga Chlorella kessleri

Deng

Xue

Chen

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND In recent years, mixotrophic culture of algal cells has been gaining increasing interest, largely because it offers an efficient means of commercial production of lipids and other value‐added products. In this work, the impacts of glucose addition on the biomass production, chemical composition and fatty acid profiles of Chlorella kessleri were investigated to exploit the relationships between extra organic carbon and changes in the distribution of chemical compositions in algal cells. RESULTS Results showed that C. kessleri grew fast in the mixotrophic cultures with a maximum algal biomass and specific growth rate of 4.46 g L−1 and 1.27 day−1, respectively. Glucose in the cultures was absorbed and utilized effectively by this alga, whose uptake efficiencies ranged from 88.2% to 98.3%, but it had a negative impact on the synthesis of photosynthetic pigments. Additionally, the culture's pH and chemical composition, including pigments, carbohydrate, protein and lipids, in this alga varied with the change of glucose concentrations in the cultures. The fatty acid profiles of this alga indicated that its biomass could be used as live feeds in aquaculture or a good‐quality feedstock for biodiesel production. CONCLUSIONS These results suggested that adding an extra organic carbon source (glucose) to a photoautotrophic culture was a potentially effective way to improve the performance in mixotrophically culture of microalgae, for example in terms of higher biomass and lipid production. © 2018 Society of Chemical Industry

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Section: Resultscontrasting

confidence: 61%

Glucose addition‐induced changes in the growth and chemical compositions of a freshwater microalga Chlorella kessleri

Deng

Xue

Chen

et al. 2018

J of Chemical Tech & Biotech

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“…Among the species of microalgae studied for this purpose, the Chlorophyceae Desmodesmus subspicatus stands out for its high productivity observed in the distillery effluent [5]. Its cultivation could be potentiated by reducing the vinasse characteristic turbidity, allowing suitable incidence of luminosity to the photosynthetic cells, therefore promoting mixotrophic growth [6].…”

Section: Introductionmentioning

confidence: 99%

Growth of Desmodesmus subspicatus green microalgae and nutrient removal from sugarcane vinasse clarified by electrocoagulation using aluminum or iron electrodes

Saavedra¹,

Bissoto²,

Souza³

et al. 2019

DYNA

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Sugarcane ethanol production generates considerable quantities of vinasse, its main wastewater. Microalgae cultivation is a promising option for effluent remediation, since the generated biomass can be feedstock for biofuel and bio-based chemical production. Due to vinasse high turbidity, pretreatment is necessary to clarify this effluent, adapting it as a mixotrophic culture medium. In this context, the present research evaluated the integrated process of electrocoagulation (EC) of sugarcane vinasse with aluminum or iron electrodes and subsequent cultivation of green microalgae Desmodesmus subspicatus. Results indicate pH neutralization and high turbidity removal efficiency by EC with both electrode materials. Aluminum EC and subsequent microalgae cultivation removed 66 and 75% of initial total organic carbon and total nitrogen, respectively, with biomass productivity of 1.45 g L-1day-1 and maximum specific growth rate of 0.095 h-1. Microalgae productivity was inferior in vinasse pretreated by iron EC, suggesting possible interference of ferric compounds in the microalgal development.

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“…The short lag phase was caused by the rapid transport and diffusion of organic carbon in the form of reducing sugars into the cell using membrane transport proteins 20 . The most assimilable hexoses, which are present in beet molasses, are rapidly and directly utilized by cells and converted into the central carbon metabolism, providing a large amount of energy in the form of ATP 11,20,21 . Zhang et al 22 also observed that, especially at a low concentration in culture medium, glucose shortened the lag phase, as it was assimilated easily during the cultivation.…”

Section: Discussionmentioning

confidence: 99%

“…The similarity of the photoheterotrophic cultivation system to mixotrophy consists in microalgal requirements for light and the utilization of organic compounds as a carbon source. In contrast to mixotrophy, microalgae cultivated in photoheterotrophic culture conditions cannot absorb carbon dioxide 11 . Photoheterotrophy is very rarely applied in comparison with mixotrophic cultivation.…”

mentioning

confidence: 99%

Agro-industrial by-product in photoheterotrophic and mixotrophic culture of Tetradesmus obliquus: Production of ω3 and ω6 essential fatty acids with biotechnological importance

Piasecka

Nawrocka

Wiącek

et al. 2020

Sci Rep

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In recent years, researchers have highlighted the role of low cost-efficient agro-industrial by-products used as supplements in algal culture media. The aim of the study was to identify and characterize the basic metabolic pathways in Tetradesmus obliquus cells induced by supplementation with beet molasses in photoheterotrophic and mixotrophic culture conditions. to assess the impact of the nutritional strategy in unicellular algae, growth curves were plotted and lipid, carbohydrate, and protein levels were determined. Fourier Transform Infrared Spectroscopy was applied to measure the Tetradesmus obliquus cell composition. Additionally, the C16-C18 fatty acid profile of Tetradesmus obliquus was determined by gas chromatograph/mass spectrometry. The switch from autotrophy to photoheterotrophy and mixotrophy contributes to shortening of the adaptation growth phase. The highest protein content was obtained in the mixotrophic growth. This study has demonstrated high content of 18:1, cisΔ 9 , 18:2, cisΔ 9,12 , ω6, and 18:3, cisΔ 9,12,15 , ω3 in photoheterotrophic and mixotrophic culture conditions. High levels of proteins and essential fatty acids make Tetradesmus obliquus cell biomass important for human and animals health. Microalgae are not only a source of many valuable bio-products e.g. proteins, carbohydrates, lipids, ω3 and ω6 fatty acids or pigments applied in many different commercial sectors but can be a simple and economical solution to wastewater treatment and waste management 1. Algal cells utilize effectively agricultural, industrial and municipal wastewaters, waste raw materials, and by-products from many branches of industry. Algae are capable of assimilating organic compounds and other ingredients for biomass production and synthesis of both basic and specific metabolites. Additionally, the use of wastewaters and waste materials make microalgal biomass and bio-product production environmentally friendly 2. A number of studies have described supplementation of the algal culture medium with agricultural waste such as dairy manure 3 , liquid waste produced in piggeries 4 , post-fermentation effluents and wastewater from fruit, vegetable or cultivated plant processing, i.e. residues of cane bagasse and pineapple peel 5 , cassava 6 , sweet sorghum 7 , or hydrolysate of Jerusalem artichoke tubers 8. Most algae are autotrophs; however, some species such as Auxenochlorella protothecoides 9 Parachlorella kessleri 10 and Tetradesmus obliquus can assimilate organic compounds. Cultivation modes based on organic carbon sources include heterotrophy, photoheterotrophy, and mixotrophy 11. Compared to the heterotrophic mode, the photoheterotrophic and mixotrophic cultivation systems have several advantages e.g. intensification of algal growth and synthesis of valuable metabolites, such as fatty acids 12. Green algae growing in photoheterotrophic and mixotrophic culture conditions are natural producers of polyunsaturated fatty acids 10,13. In terms of human nutrition, one of the most important PUFAs are essential fatty...

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Mixotrophic cultivation, a preferable microalgae cultivation mode for biomass/bioenergy production, and bioremediation, advances and prospect

Cited by 213 publications

References 136 publications

Glucose addition‐induced changes in the growth and chemical compositions of a freshwater microalga Chlorella kessleri

Glucose addition‐induced changes in the growth and chemical compositions of a freshwater microalga Chlorella kessleri

Growth of Desmodesmus subspicatus green microalgae and nutrient removal from sugarcane vinasse clarified by electrocoagulation using aluminum or iron electrodes

Agro-industrial by-product in photoheterotrophic and mixotrophic culture of Tetradesmus obliquus: Production of ω3 and ω6 essential fatty acids with biotechnological importance

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