Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen Prevotellaceae UCG-004 in Hu sheep

Li, Chuang; Chen, Ning; Zhang, Xingxing; Shahzad, Khuram; Qi, Ruxin; Zhang, Zhenbin; Lu, Zhiqi; Lü, Yue; Yu, Xiao; Zafar, Muhammad Mubashar; Wang, Mengzhi; Liu, Wujun

doi:10.3389/fmicb.2022.978940

Cited by 13 publications

(14 citation statements)

References 63 publications

(55 reference statements)

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“…The gatY , uidA , and rbsD genes were associated with carbohydrate metabolism process in BP (Figure 3C), such as ascorbate and aldarate metabolism, porphyrin metabolism, pentose and glucuronate interconversions, galactose metabolism (Figure 3E), which were involved in the fight against oxidative stress. [ 30 ] Next, we focused on the downregulated genes. The top 3 most downregulated genes ( OEJ30_003168 , OEJ30_004227 , and OEJ30_001305 ) were related to the transport of amino acids by transfer RNA, [ 31 ] indicating impaired protein synthesis after phage@Pd treatment.…”

Section: Resultsmentioning

confidence: 99%

Microenvironment‐Activated Nanozyme‐Armed Bacteriophages Efficiently Combat Bacterial Infection

et al. 2023

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Bacterial infection is one of the greatest challenges to public health, requiring new therapeutic methods. Herein, an innovative nanozyme‐armed phage (phage@palladium (Pd)) system is fabricated for combating bacterial infection. The proposed phage@Pd preserves the function of the phages to achieve precise recognition and adhesion to the host Escherichia coli. In aid of the phages, the ultrasmall Pd nanozymes equipped with conspicuous pH‐dependent peroxidase‐like activity can generate toxic hydroxyl radical around the bacteria in acidic and hydrogen‐peroxide‐overexpressed infection microenvironment while remaining inert in physiological conditions, thus realizing the noteworthy elimination of bacteria at infected sites, and in the meantime ensuring the biological safety of phage@Pd in healthy tissues. In addition, the filamentous structure of phage@Pd can also enhance its bactericidal efficiency toward nonhost bacteria by randomly entangling on them, indicating possible broad‐spectrum germicidal efficacy. Notably, phage@Pd can not only eradicate planktonic bacteria, but also kill the bacteria inside the biofilm in vitro. For both in vivo models of acute bacterial pneumonia or subcutaneous abscess, phage@Pd shows significant activity in eliminating infection and promoting tissue recovery. These results demonstrate that the phage@Pd nanohybrid is a safe and effective antimicrobial agent, providing a new insight into development of advanced antibacterial materials.

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Section: Resultsmentioning

confidence: 99%

Microenvironment‐Activated Nanozyme‐Armed Bacteriophages Efficiently Combat Bacterial Infection

et al. 2023

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“…Moreover, SCFAs has also been shown to regulate energy intake, regulate cell apoptosis and decrease cholesterol ( Murugesan et al, 2018 ; Prasad and Bondy, 2018 ; Yang J. et al, 2022 ; Yang X. et al, 2022 ). Prevotellaceae in the intestine could digest pectin, hemicellulose and high carbohydrate foods, indicating its key roles in digestion and absorption ( Li A. et al, 2022 ; Li C. et al, 2022 ). Alloprevotella could secrete acetate and succinate and these beneficial metabolites are critical for intestinal homeostasis and decreased cardiovascular disease risk ( Yuan et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…However, seaweed polysaccharide can maintain the gut microbial balance and this may be one of the modes of action of seaweed polysaccharides. Microorganisms inhabiting the intestine could interact in a synergistic, antagonistic or symbiotic relationship to form a stable intestinal environment ( Li A. et al, 2022 ; Li C. et al, 2022 ). In this study, we observed significant correlations among some bacteria through correlation network analysis.…”

Section: Discussionmentioning

confidence: 99%

Seaweed polysaccharide relieves hexavalent chromium-induced gut microbial homeostasis

Z²,

Wang³

et al. 2023

Front. Microbiol.

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Heavy metals released in the environment pose a huge threat to soil and water quality, food safety and public health. Additionally, humans and other mammals may also be directly exposed to heavy metals or exposed to heavy metals through the food chain, which seriously threatens the health of animals and humans. Chromium, especially hexavalent chromium [Cr (VI)], as a common heavy metal, has been shown to cause serious environmental pollution as well as intestinal damage. Thus, increasing research is devoted to finding drugs to mitigate the negative health effects of hexavalent chromium exposure. Seaweed polysaccharides have been demonstrated to have many pharmacological effects, but whether it can alleviate gut microbial dysbiosis caused by hexavalent chromium exposure has not been well characterized. Here, we hypothesized that seaweed polysaccharides could alleviate hexavalent chromium exposure-induced poor health in mice. Mice in Cr and seaweed polysaccharide treatment group was compulsively receive K2Cr2O7. At the end of the experiment, all mice were euthanized, and colon contents were collected for DNA sequencing analysis. Results showed that seaweed polysaccharide administration can restore the gut microbial dysbiosis and the reduction of gut microbial diversity caused by hexavalent chromium exposure in mice. Hexavalent chromium exposure also caused significant changes in the gut microbial composition of mice, including an increase in some pathogenic bacteria and a decrease in beneficial bacteria. However, seaweed polysaccharides administration could ameliorate the composition of gut microbiota. In conclusion, this study showed that seaweed polysaccharides can restore the negative effects of hexavalent chromium exposure in mice, including gut microbial dysbiosis. Meanwhile, this research also lays the foundation for the application of seaweed polysaccharides.

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“…Prevotella was more abundant in subjects with a high inflammatory index ( Aranaz et al, 2021 ). Prevotellaceae UCG-004 increased production of the butyric acid significantly upregulated the metabolism of ascorbate and aldarate metabolism, thereby improving the antioxidant properties of Hu sheep ( Li C. et al, 2022 ). In our study, XZP increased the relative abundance of Prevotellaceae UCG-001 genus in hamster feces induced by high fat diet.…”

Section: Discussionmentioning

confidence: 99%

Metabolome combined with gut microbiome revealed the lipid-lowering mechanism of Xuezhiping capsule on hyperlipidemic hamster induced by high fat diet

Wang

Zhang²,

Luo³

et al. 2023

Front. Mol. Biosci.

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Introduction: Hyperlipidemia is a common metabolic disorder with presence of excess fat or lipids in the blood, may induce liver injury, oxidative stress and inflammatory. Xuezhiping capsule (XZP) is a famous Chinese patent medicine clinically used for anti-hyperlipidemia. However, the regulation mechanism of XZP on hyperlipidemia has not been elucidated so far.Methods: This study aimed to explore the effects of XZP on hypolipidemic, antioxidant and anti-inflammatory effects, and the potential mechanism by a combination of untargeted metabolomics and 16S rRNA sequencing.Results: The results indicated that XZP reduced the level of total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), increased the level of high density liptein cholesterol (HDL-C), alleviated excessive accumulation of lipid droplets in liver. Biochemical indexes of liver function including gamma glutamyl transferase (GGT) and glutamic oxaloacetic transaminase (GOT) in liver were remarkably decreased. Meanwhile, XZP increased the level of oxidative stress biochemical indexes including superoxide dismutase (SOD) and glutathione (GSH). In addition, XZP increased the level of peroxisome proliferators-activated receptors α (PPARα), acetyl CoA carboxylase 1 (ACOX1) and cholesterol 7-alpha hydroxylase (CYP7A1) in liver, and improved lipid metabolism in serum, liver and fecal lipid metabolism. XZP increased diversity index and the ratio of Firmicutes and Bacteroidetes, regulated seventeen genera, and illustrated strong correlations with liver lipid metabolism and phenotypic indicators.Discussion: These findings suggest that XZP reduced blood lipid and liver lipid, protected liver function, anti inflammation and anti-oxidation, ameliorate lipid metabolic disorders by modulating alpha linolenic acid and linoleic acid metabolism, bile acid metabolism, arachidonic acid metabolism, and regulated gut microbiota composition of high-fat diet (HFD) hamsters.

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Mixed silage with Chinese cabbage waste enhances antioxidant ability by increasing ascorbate and aldarate metabolism through rumen Prevotellaceae UCG-004 in Hu sheep

Cited by 13 publications

References 63 publications

Microenvironment‐Activated Nanozyme‐Armed Bacteriophages Efficiently Combat Bacterial Infection

Microenvironment‐Activated Nanozyme‐Armed Bacteriophages Efficiently Combat Bacterial Infection

Seaweed polysaccharide relieves hexavalent chromium-induced gut microbial homeostasis

Metabolome combined with gut microbiome revealed the lipid-lowering mechanism of Xuezhiping capsule on hyperlipidemic hamster induced by high fat diet

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