Mixed reverse micellar systems for extraction and purification of β-glucosidase

Hemavathi, A. B.; Hebbar, H. Umesh; Raghavarao, K.S.M.S.

doi:10.1016/j.seppur.2009.11.026

Cited by 34 publications

(27 citation statements)

References 27 publications

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“…Several reports on the application of RME for separation and purification of biomolecules are available for model systems (4)(5)(6)(7)(8). In recent years, a few studies on RME of biomolecules from natural=real systems have been reported (9)(10)(11)(12)(13)(14)(15).…”

Section: Introductionmentioning

confidence: 99%

Reverse Micellar Extraction of Bromelain from Pineapple (Ananas comosusL. Merryl) Waste: Scale-up, Reverse Micelles Characterization and Mass Transfer Studies

Hebbar

Hemavathi

Sumana

et al. 2011

Separation Science and Technology

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Scale-up studies for phase transfer mode of reverse micellar extraction are attempted for the separation and primary purification of bromelain (EC 3.4.22.33) from pineapple (Ananas comosus L. Merryl) waste. Characterization of reverse micelles and mass transfer studies for the real system has been attempted for the first time. Scale-up of the extraction process employing commercial grade surfactant cetyltrimethylammonium bromide (CTAB) and solvent isooctane resulted in purification of 2.43 fold with an activity recovery 81.3%. The reverse micellar size estimated using empirical and geometrical models indicated that the reverse micelles are large enough (R m ¼ 7.2-9.6 nm) to host bromelain molecules that are relatively smaller in size ($1.67 nm). The studies on the kinetics of mass transfer indicated a relatively slower rate (by $34%) of mass transfer in case of back extraction compared to forward extraction. Process scale-up did not significantly affect the extraction efficiency whereas purity of phase components played a major role. The mass transfer across the phases was high in the initial period of mixing for both forward and back extractions.

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Section: Introductionmentioning

confidence: 99%

Reverse Micellar Extraction of Bromelain from Pineapple (Ananas comosusL. Merryl) Waste: Scale-up, Reverse Micelles Characterization and Mass Transfer Studies

Hebbar

Hemavathi

Sumana

et al. 2011

Separation Science and Technology

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“…The presence of zwitterion surfactant was predicted to weaken the surface charge density at the interfacial region hence decreased the inhibitory power of AOT on enzyme. 17 Previous researchers reported that strong electrostatic interaction between ionic surfactants polar head and protein charges could lead to protein unfolding. 18 Therefore, to weaken the interaction, zwitterion surfactant molecule was introduced to facilitate the solubilization of erythromycin in the organic phase.…”

Section: Effect Of the Aot Concentrationmentioning

confidence: 99%

The solubilization and extraction parameters of erythromycin from aqueous phase into mixed AOT/SB3-18 reverse micelle phase

Aziz

Sakinah

2017

Chem. Eng. Res. Bull.

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A liquid anionic-zwitterionic surfactant-based aqueous two-phase extraction was developed and applied for the extraction of erythromycin. Erythromycin solubilization from aqueous to reverse micelle phase was studied. Zwitterion SB3-18 surfactant was added to ionic solution of bis(2-ethylhexyl) sulfosuccinate sodium salt (AOT) to form an organic mixed micellar phase. Erythromycin was then added to the mixtures and two clear phases were formed. The parameters affecting erythromycin solubilization including AOT concentration (20.0 ̶ 120.0 g/L), zwitterion concentration (6.0 ̶ 16.0 g/L), NaCl concentration (0.0 ̶ 25.0 g/L) and aqueous pH (6.0 ̶ 9.0) were investigated via statistical software. Solubilization of erythromycin into mixed reverse micelle could be easily evaluated by the measurement of erythromycin concentration in the organic phase. The results showed that AOT and zwitterion concentration governed the erythromycin solubilization by a factor of 4.562 based on the Pareto chart.

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“…Absorbance of pnitrophenol formed by the enzyme reaction was measured. One unit of β-glucosidase activity was defined as the amount of enzyme that released 1 nmol of p-nitrophenol per min under standard conditions of 37 o C and pH 5.0 for 15 min [29].…”

Section: Enzyme Assaymentioning

confidence: 99%

Membrane processing for purification and concentration of β-glycosidases from barley (Hordeum vulgare)

Hemavathi

Raghavarao

2011

Biotechnol Bioproc E

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Tangential flow ultrafiltration with a polyethersulfone (100 kDa) membrane was used for the purification and concentration of β-glycosidases (β-galactosidase and β-glucosidase) from aqueous extract of barley. The performances of mode 1 (concentration followed by diafiltration) and mode 2 (diafiltration followed by concentration) were compared. In mode 1 activity recoveries of 91.44 and 88% as well as purifications of 1.84 and 1.77-fold for β-galactosidase and β-glucosidase, respectively, were obtained in a total processing time of about 9 h. In mode 2 activity recoveries of 95.68 and 91.76% with purifications of 4.56 and 4.38-fold for β-galactosidase and β-glucosidase, respectively, were obtained in a total processing time of about 6 h. The removal of total carbohydrates and protein was 56.74 and 50.73%, respectively, in mode 1, whereas it was 81.46 and 79.04%, respectively, in mode 2. The diafiltration volume and volume concentration of 3 were maintained in both mode 1 and 2. Flux decline was severe in mode 1 and led to a long processing time of about 9 h. These results indicate that mode 2 was better than mode 1 for purification of β-glycosidases.

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Mixed reverse micellar systems for extraction and purification of β-glucosidase

Cited by 34 publications

References 27 publications

Reverse Micellar Extraction of Bromelain from Pineapple (Ananas comosusL. Merryl) Waste: Scale-up, Reverse Micelles Characterization and Mass Transfer Studies

Reverse Micellar Extraction of Bromelain from Pineapple (Ananas comosusL. Merryl) Waste: Scale-up, Reverse Micelles Characterization and Mass Transfer Studies

The solubilization and extraction parameters of erythromycin from aqueous phase into mixed AOT/SB3-18 reverse micelle phase

Membrane processing for purification and concentration of β-glycosidases from barley (Hordeum vulgare)

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