Mixed hybrid bilayer lipid membranes on mechanically polished titanium surface

Mixed Silane‐based Self‐assembled Monolayers Deposited on Fluorine Doped Tin Oxide as Model System for Development of Biosensors for Toxin Detection

“…On the contrary, the pre‐hydrated FTO substrates lead to a formation of mhBLMs on SAM−P2 substrates containing up to 80 % of MTS back‐filler (Figure 4, filled circles). Complex capacitance values in the range from 1.1±0.1 μF cm −2 to 0.8±0.1 μF cm −2 (Figure 4 B−F, filled circles) are comparable to those obtained on Au [45] or Ti [38, 46] thus, indicate successful vesicle fusion and lipid layer formation. Figure 5 clearly illustrates significant differences in properties of mhBLMs obtained on pre‐hydrated and non‐hydrated FTO substrates.…”

“…In the current study, following the methodology, which was recently reported for the assembly of mixed hybrid bilayer membranes (mhBLM) on metallurgic titanium surface [38], we attempted to assemble mixed self‐assembled silane monolayers on fluorine doped tin oxide conducting films. The mixed monolayer consisting of methyltrichlorosilane (MTS) as a short chain spacer and octadecyltrichlorosilane (OTS) serving as a long alkyl chain anchor would allow formation of less compact bilayer providing enough flexibility in the phospholipid layer sufficient for functional reconstitution of membrane proteins (peptides), in particular, melittin.…”

Streamlined Fabrication of Hybrid Lipid Bilayer Membranes on Titanium Oxide Surfaces: A Comparison of One- and Two-Tail SAM Molecules

“…Sabirovas et al further investigated the effects of the organic solvent used for surface cleaning and of heating the surface prior to silanization on the resulting SAM properties, and demonstrated HLB formation on the silanized SAM with phospholipids containing 40 mol% cholesterol along with reusability of the silanized SAM [ 26 ]. It has also been shown that a silanized SAM could be formed with a mixture of long and short silanes to create aqueous reservoirs below the short silanes, which can potentially be useful for protein reconstitution into the HLB [ 27 ]. Within this context, tethered lipid bilayers [ 28 ]—a specific type of HLB—are also noteworthy, and the use of silanization to directly tether phospholipids to titanium can result in a silane-tethered phospholipid SAM, onto which another layer of phospholipid can assemble to form an HLB [ 29 ].…”

“…Within this context, tethered lipid bilayers [ 28 ]—a specific type of HLB—are also noteworthy, and the use of silanization to directly tether phospholipids to titanium can result in a silane-tethered phospholipid SAM, onto which another layer of phospholipid can assemble to form an HLB [ 29 ]. Collectively, these studies have shown the possibility of HLB formation on titanium; however, there are also challenges such as the need for additional steps to prepare the surface (e.g., vapor deposition [ 25 ] or mechanical polishing [ 26 , 27 ]), extensive use of organic solvents during silanization, high temperature, and long fabrication time, all of which can limit HLB practicality in terms of fabrication and application.…”

Techno-economic design requirements for integrating biosensors in environmental monitoring of aquatic ecosystems

“…Voltage-induced poration has been utilized in the monitoring of gating phenomena (Rems et al, 2020), and grafting-driven poration have been proposed for clinical application (Duan et al, 2017). The formation of asymmetric membranes (Shukla et al, 2019) or mixed lipid membranes (Sabirovas et al, 2020) can provide some degree of poration and has been proposed for signal amplification and decrease of detection levels. More mechanismic strategies include the guiding of some kind of charge accumulation at domains on the membrane surface and the use of lipid mixtures that can accommodate these accumulations as pores.…”