Mixed-genotype infections of Trichoplusia ni larvae with Autographa californica multicapsid nucleopolyhedrovirus: Speed of action and persistence of a recombinant in serial passage

Zwart, Mark P.; Werf, Wopke Van der; Georgievska, Liljana; Oers, Monique M. van; Vlak, Just M.; Cory, Jenny S.

doi:10.1016/j.biocontrol.2009.10.002

Cited by 11 publications

(10 citation statements)

References 32 publications

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“…Transmission of virus recombinants also depends on the competitive interaction between wild‐type and genetically modified virus within the host insect. Results of Zwart et al. (2009b, 2010) indicate that the competitive fitness of egt ‐deletion variants within individual insect hosts is reduced.…”

Section: Discussionmentioning

confidence: 99%

“…(2001) reported unaltered within‐host fitness, when a recombinant AcMNPV expressing the insect‐specific toxin AaIT was in direct competition with its parental wild‐type virus. Models can help to assess these interactions (Bianchi et al., 2002; Bonsall et al., 2005; Sun et al., 2006; Zwart et al., 2009b, 2010). A critical parameter that may be needed in models for competitive fitness of baculovirus genotypes at agro‐ecosystem level is the transmission of virus genotypes from hosts that die from mixed infections.…”

Section: Discussionmentioning

confidence: 99%

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Effects of single and mixed infections with wild type and genetically modified Helicoverpa armigera nucleopolyhedrovirus on movement behaviour of cotton bollworm larvae

Georgievska

Joosten

Hoover

et al. 2010

Entomologia Exp Applicata

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Naturally occurring insect viruses can modify the behaviour of infected insects and thereby modulate virus transmission. Modifications of the virus genome could alter these behavioural effects. We studied the distance moved and the position of virus-killed cadavers of fourth instars of Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) infected with a wild-type genotype of H. armigera nucleopolyhedrovirus (HaSNPV) or with one of two recombinant genotypes of this virus on cotton plants. The behavioural effects of virus infection were examined both in larvae infected with a single virus genotype, and in larvae challenged with mixtures of the wild-type and one of the recombinant viruses. An egt-negative virus variant caused more rapid death and lower virus yield in fourth instars, but egt-deletion did not produce consistent behavioural effects over three experiments, two under controlled glasshouse conditions and one in field cages. A recombinant virus containing the AaIT-(Androctonus australis Hector) insect-selective toxin gene, which expresses a neurotoxin derived from a scorpion, caused faster death and cadavers were found lower down the plant than insects infected with unmodified virus. Larvae that died from mixed infections of the AaIT-expressing recombinant and the wild-type virus died at positions significantly lower, compared to infection with the pure wild-type viral strain. The results indicate that transmission of egt-negative variants of HaSNPV are likely to be affected by lower virus yield, but not by behavioural effects of egt gene deletion. By contrast, the AaIT recombinant will produce lower virus yields as well as modified behaviour, which together can contribute to reduced virus transmission under field conditions. In addition, larvae infected with both the wild-type virus and the toxin recombinant behaved as larvae infected with the toxin recombinant only, which might be a positive factor for the risk assessment of such toxin recombinants in the environment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effects of single and mixed infections with wild type and genetically modified Helicoverpa armigera nucleopolyhedrovirus on movement behaviour of cotton bollworm larvae

Georgievska

Joosten

Hoover

et al. 2010

Entomologia Exp Applicata

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“…These disadvantages limit the use of baculoviruses on an even wider scale and have contributed to the focus of research on baculovirus engineering for improved insecticidal properties over the last couple of decades. Previous studies revealed that through genetic engineering, the envisaged insecticidal properties of baculoviruses, such as increased speed of action, enhanced virulence and extended host range, might be achieved Zwart et al, 2010;Tang et al, 2011) but awaits public acceptance.…”

Section: Host Rangementioning

confidence: 99%

“…specific toxin (Tang et al, 2011;Gramkow Chapter 1 10 et al, 2010), hormone or enzyme (ii) deletion of a baculovirus gene e.g. the ecdysteroid glucosyltransferase or egt gene Zwart et al, 2010;Erlandson, 2009).…”

Section: A Speed Of Kill and Virus Yield (Or Productivity)mentioning

confidence: 99%

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Diversity and biological activity of nucleopolyhedroviruses of the leafworm Spodoptera litura

Ali

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Spodoptera litura is an emerging insect pest in a wide range of crops worldwide. The insect is difficult to control because of resistance development to synthetic insecticides and emerging resistance to Bacillus thuringiensis toxins. Therefore there is a need to develop biological control agents, preferably from an indigenous source to avoid risks associated with importation of exotic natural antagonists. A Pakistani isolate of S. litura nucleopolyhedrovirus (SpltNPV, Baculoviridae), SpltNPV-Pak-BNG, was obtained from the field and characterized biologically and genetically, and compared to a SpltNPV reference isolate, SpltNPV-G1, thought to be of Chinese origin. The dose mortality response (LD50) of SpltNPV-Pak-BNG was not significantly different from that of the reference isolate SpltNPV-G1, but the time-to-death (LT50) was significantly shorter for SpltNPV-Pak-BNG than for SpltNPV-G1. DNA restriction enzyme profiling indicated that SpltNPV-G1 and SpltNPV-Pak-BNG are different viruses. Sequence analysis of 'ORF24', specific for SpltNPV (and S. littoralis NPV as ORF21), and the conserved baculovirus core genes polyhedrin, DNApol, pif-2 and lef-8 confirmed that this was indeed the case and that SpltNPV-Pak-BNG is a genuine SpltNPV variant whereas the SpltNPV-G1 isolate we used is in fact a SpliNPV variant, renamed to SpliNPV-G1. The newly isolated SpltNPV-Pak-BNG has potential for development as a biocontrol agent of S. litura in Pakistan.

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Genotype assembly, biological activity and adaptation of spatially separated isolates of Spodoptera litura nucleopolyhedrovirus

Ali

Abma-Henkens

Werf

et al. 2018

Journal of Invertebrate Pathology

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Mixed-genotype infections of Trichoplusia ni larvae with Autographa californica multicapsid nucleopolyhedrovirus: Speed of action and persistence of a recombinant in serial passage

Cited by 11 publications

References 32 publications

Effects of single and mixed infections with wild type and genetically modified Helicoverpa armigera nucleopolyhedrovirus on movement behaviour of cotton bollworm larvae

Effects of single and mixed infections with wild type and genetically modified Helicoverpa armigera nucleopolyhedrovirus on movement behaviour of cotton bollworm larvae

Diversity and biological activity of nucleopolyhedroviruses of the leafworm Spodoptera litura

Genotype assembly, biological activity and adaptation of spatially separated isolates of Spodoptera litura nucleopolyhedrovirus

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