Mixed culture polyhydroxyalkanoate (PHA) production from volatile fatty acid (VFA)-rich streams: Effect of substrate composition and feeding regime on PHA productivity, composition and properties

Albuquerque, Maria G.E.; Martino, Verónica P.; Pollet, Éric; Avérous, Luc; Reis, Maria A.M.

doi:10.1016/j.jbiotec.2010.10.070

Cited by 264 publications

(154 citation statements)

References 46 publications

(77 reference statements)

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“…Compared to pure cultures, the use of mixed culture enriched under the Aerobic Dynamic Feeding or Feast and Famine conditions is more convenient. The advantage of using mixed cultures is the production of co-polymers with a broad range of PHA composition depending on the substrate used due to their metabolic capacities to utilize a wider range of substrates [8,40]. Moreover, mixed cultures have better survival under stringent environmental conditions and variations occurring specially in biowaste feed, thus, providing an economical option by eliminating the need to sterilize the feed [17,[41][42][43][44][45].…”

Section: Bacterial Pha Synthasesmentioning

confidence: 99%

“…Interestingly, the non-PHB yield may be higher in content with acidogenic effluents in comparison to synthetic substrates. P(3HB:3HV) may be formed with HV content in the range 15-31 %, varying linearly with the amount of propionate and valerate [8]. The enriched cultures of glycogen accumulating organisms were reported to utilize VFA from fermented sugarcane molasses to produce copolymers having 3HB (56-70 mol%), 3HV (13-43 mol%), 3HHx (1-23 mol%), 3H2 MB (0-2 mol%) and 3H2MV (0-2 mol%) which varied with VFA composition [7].…”

Section: Substrate and Feeding Regimementioning

confidence: 99%

“…With fermented molasses as source of VFAs being fed continuously, relative to pulse wise feeding resulted in enhancing the 3HV content in PHA from 31 to 39 %. The lower yield of 3HV in PHA may be due to the depletion of precursors at the end of each pulse [8,47]. It resulted in the formation of only PHB.…”

Section: Substrate and Feeding Regimementioning

confidence: 99%

“…Interestingly, P(3HB:3HHx) with 3HHx equal to 10-12 mol% has quite lower values (T m : 96-127°C) in comparison to P(3HB:10-20 % 3HV) (T m : 137-156°C) and P(3HB:16 % 4HB) (T m : 150°C) having relatively similar content of 3HB. This may be due the presence of mcl-HA 3HHx [7,8,30,65,74,80]. Tercopolymer P(3HB:3HV:4HB) with higher 3HB content e.g.…”

Section: Thermal Propertiesmentioning

confidence: 99%

“…These aspects in PHAs are affected by the type of bacteria, substrate, feeding strategies and physiological conditions. All these components can be manipulated to attain polymer with desirable properties [6][7][8]. PHAs are divided into different classes based on the carbon (C) chain length: (1) short (scl-PHAs; 3-5Cs), (2) medium (mcl-PHAs; 6-14Cs), and (3) long (lcl-PHAs; 17-18Cs).…”

Section: Introductionmentioning

confidence: 99%

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Challenges and Opportunities for Customizing Polyhydroxyalkanoates

et al. 2015

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Polyhydroxyalkanoates (PHAs) as an alternative to synthetic plastics have been gaining increasing attention. Being natural in their origin, PHAs are completely biodegradable and eco-friendly. However, consistent efforts to exploit this biopolymer over the last few decades have not been able to pull PHAs out of their nascent stage, inspite of being the favorite of the commercial world. The major limitations are: (1) the high production cost, which is due to the high cost of the feed and (2) poor thermal and mechanical properties of polyhydroxybutyrate (PHB), the most commonly produced PHAs. PHAs have the physicochemical properties which are quite comparable to petroleum based plastics, but PHB being homopolymers are quite brittle, less elastic and have thermal properties which are not suitable for processing them into sturdy products. These properties, including melting point (T m ), glass transition temperature (T g ), elastic modulus, tensile strength, elongation etc. can be improved by varying the monomeric composition and molecular weight. These enhanced characteristics can be achieved by modifications in the types of substrates, feeding strategies, culture conditions and/or genetic manipulations.

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