Mixed cationic/anionic surfactants for semipermanent wall coatings in capillary electrophoresis

Wang, Chuanzhong; Lucy, Charles A.

doi:10.1002/elps.200305760

Cited by 70 publications

(60 citation statements)

References 49 publications

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“…Two methods, electrokinetic rinsing and hydrodynamic rinsing, were used to evaluate the coating stability. The electrokinetic rinsing method, also known as successive injection method, has been widely used to study the stability of capillary coatings [30][31][32][33]. In electrokinetic rinsing method, after the capillary was coated and the excess gemini was flushed out of the capillary, the EOF was con-secutively determined without regenerating or refreshing the capillary.…”

Section: Coating Stability Testmentioning

confidence: 99%

Long‐chained gemini surfactants for semipermanent wall coatings in capillary electrophoresis of proteins

Liu

Jie

et al. 2008

Electrophoresis

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In this paper, we presented the first example of using gemini surfactants as semipermanent coatings in CE for protein separation. These coatings are based on the self-assembly of a series of cationic gemini surfactants, alkanediyl-alpha,omega-bis(dimethylalkylammonium bromide) (m-s-m), on the capillary wall. The coatings can keep stable for a long time without surfactant in the buffer, e.g., after the surfactants were removed from the buffer, the reversed EOF only decreased by 3.6 and 3.9% for 18-2-18 and 16-2-16 coatings over 60 min under continuous electrophoretic conditions. The coating stability increased with the alkyl chain length m. The double long chains of geminis (m > or = 14) yielded a good coating stability; meanwhile, the spacer group acted as an EOF modifier. Thus, this bifunctional surfactant coating provided a new buffer-independent method for EOF control. For 18-s-18 series, the best coating stability and largest EOF were obtained at s = 10. Ranging s from 3 to 10 yielded a linear fine-tuning of EOF and thereby allowed the adjustment of the protein apparent mobility. Highly efficient separation (>500 000 plates/m) was achieved with all the 18-s-18 coatings. Excellent run-to-run and day-to-day reproducibility (RSD of migration time

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Section: Coating Stability Testmentioning

confidence: 99%

Long‐chained gemini surfactants for semipermanent wall coatings in capillary electrophoresis of proteins

Liu

Jie

et al. 2008

Electrophoresis

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“…Some of the different applications suggested are described below. Some possible applications where only single studies have been made, such as the use of catanionic surfactant mixtures for wall coatings in capillary electrophoreses (Wang & Lucy 2004) and for solubilization of fullerenes (Li et al 2006), have not been included.…”

Section: Applicationsmentioning

confidence: 99%

Pharmaceutical applications for catanionic mixtures

Bramer

Dew

Edsman

2007

Journal of Pharmacy and Pharmacology

120

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Mixtures of oppositely charged surfactants, so called catanionic mixtures, are a growing area of research. These mixtures have been shown to form several different types of surfactant aggregates, such as micelles of various forms and sizes, and lamellar structures, such as vesicles. In this review, a short introduction to the field of catanionic mixtures is presented and the pharmaceutical possibilities offered by such mixtures are reviewed. There are several interesting ideas on how to apply catanionic mixtures to improve the delivery of, for example, drug compounds and DNA, or for HIV treatment.

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“…Similar zwitterionic surfactants 1-palmitoyl-2-oleyol-sn-glycero-3-phosphocholine [54] and 1,2-dioleyol-sn-glycero-3-phosphocholine [55] were also used as a dynamic coating, the latter after a free-radical-initiated polymerization. A dynamic coating based on mixed cationic/anionic surfactants (CTAB and SDS) has also been prepared [56].…”

Section: Dynamic Wall Coatingmentioning

confidence: 99%

Capillary electrophoresis of proteins 2003–2005

Dolnı́k¹

2006

Electrophoresis

139

117

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This review article with 304 references describes recent developments in CE of proteins, and covers the two years since the previous review (Hutterer, K., Dolník, V., Electrophoresis 2003, 24, 3998-4012) through Spring 2005. It covers topics related to CE of proteins, including modeling of the electrophoretic migration of proteins, sample pretreatment, wall coatings, improving separation, various forms of detection, special electrophoretic techniques such as affinity CE, CIEF, and applications of CE to the analysis of proteins in real-world samples including human body fluids, food and agricultural samples, protein pharmaceuticals, and recombinant protein preparations.

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Mixed cationic/anionic surfactants for semipermanent wall coatings in capillary electrophoresis

Cited by 70 publications

References 49 publications

Long‐chained gemini surfactants for semipermanent wall coatings in capillary electrophoresis of proteins

Long‐chained gemini surfactants for semipermanent wall coatings in capillary electrophoresis of proteins

Pharmaceutical applications for catanionic mixtures

Capillary electrophoresis of proteins 2003–2005

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