Mix-and-read, one-minute SARS-CoV-2 diagnostic assay: development of PIFE-based aptasensor

Lee, J Michelle; Kim, Chae Rin; Kim, Sion; Min, Junhong; Lee, SangWook

doi:10.1039/d1cc04066a

Cited by 12 publications

(10 citation statements)

References 33 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The qPCR signal was shown through the cycle threshold (Ct) value, and the system could detect the lowest concentration was 37.5 pg/mL. Lee et al [ 163 ] proposed another novel protein-induced fluorescence enhancement (PIFE) based aptasensor to allow mix-and-read, one-step, and 1-min detection of SARS-CoV-2 nucleocapsid protein. In the PIFE strategy, the excited state of Cy3 can exist as a trans or cis isomer, but only the trans has a significant fluorescence quantum yield.…”

Section: Viral Proteins and Their Detectionmentioning

confidence: 99%

Aptamer-based biosensors for virus protein detection

Lou¹,

Liu²,

Shi³

et al. 2022

TrAC Trends in Analytical Chemistry

View full text Add to dashboard Cite

Section: Viral Proteins and Their Detectionmentioning

confidence: 99%

Aptamer-based biosensors for virus protein detection

Lou¹,

Liu²,

Shi³

et al. 2022

TrAC Trends in Analytical Chemistry

View full text Add to dashboard Cite

“…Given biological samples may contain molecules that potentially interfere with aptamer‐target binding, sandwich‐type interactions are particularly attractive in terms of potentially mitigating this interference. Two other labs have now utilized the aptamers from this work to create diagnostic tests for COVID‐19 (as discussed in Section 6) [94,95] …”

Section: Aptamers For the N Protein Of Sars‐cov‐2mentioning

confidence: 99%

“…Two other labs have now utilized the aptamers from this work to create diagnostic tests for COVID-19 (as discussed in Section 6). [94,95] Kang et al have also developed several N protein specific aptamers in a recent study using a DNA library containing a 53nt random region. [90] These aptamers were isolated using a previously developed SELEX method called H-sandwich SELEX.…”

Section: Aptamers For the N Protein Of Sars-cov-2mentioning

confidence: 99%

Aptamers for SARS‐CoV‐2: Isolation, Characterization, and Diagnostic and Therapeutic Developments

Amini

Zhang

et al. 2022

Analysis & Sensing

View full text Add to dashboard Cite

The SARS‐CoV‐2 virus and COVID‐19 pandemic continue to demand effective diagnostic and therapeutic solutions. Finding these solutions requires effective molecular recognition elements. Nucleic acid aptamers represent a possible solution. Characterized by their high affinity and specificity, aptamers can be rapidly identified from random‐sequence nucleic acid libraries. Over the past two years, many labs around the world have rushed to create diverse aptamers that target two important structural proteins of SARS‐CoV‐2: the spike (S) protein and nucleocapsid (N) protein. These have led to the identification of many aptamers that show real promise for the development of diagnostic tests and therapeutic agents for SARS‐CoV‐2. Herein we review all these developments, with a special focus on the development of diverse aptasensors for detecting SARS‐CoV‐2. These include electrochemical and optical sensors, lateral flow devices, and aptamer‐linked immobilized sorbent assays.

show abstract

“…In close proximity to a protein, Cy3 is preferentially locked into its trans conformational state, resulting in fluorescence enhancement. Reproduced with permission from [ 62 ] , Copyright 2022 , Elsevier …”

Section: Advances In Functional Nucleic Acid-based Technology To Over...mentioning

confidence: 99%

“…For the sake of simplifying steps, protein-induced fluorescence enhancement (PIFE), a distance-dependent liquid platform fluorescence enhancement technique, offers higher spatial resolution and specificity compared to fluorescence resonance energy transfer (FRET) for the detection of direct protein-DNA binding. Lee et al [ 62 ] integrated the PIFE method with an aptamer as a 1-min, one-step accurate assay system for SARS-CoV-2 N protein, which is more sensitive and specific than conventional antibody testing (Fig. 3D ).…”

Section: Advances In Functional Nucleic Acid-based Technology To Over...mentioning

confidence: 99%

Recent advances of functional nucleic acid-based sensors for point-of-care detection of SARS-CoV-2

Zhang

Feng

et al. 2022

Microchim Acta

View full text Add to dashboard Cite

This review focuses on critical scientific barriers that the field of point-of-care (POC) testing of SARS-CoV-2 is facing and possible solutions to overcome these barriers using functional nucleic acid (FNA)-based technology. Beyond the summary of recent advances in FNA-based sensors for COVID-19 diagnostics, our goal is to outline how FNA might serve to overcome the scientific barriers that currently available diagnostic approaches are suffering. The first introductory section on the operationalization of the COVID-19 pandemic in historical view and its clinical features contextualizes essential SARS-CoV-2-specific biomarkers. The second part highlights three major scientific barriers for POC COVID-19 diagnosis, that is, the lack of a general method for (1) designing receptors of SARS-CoV-2 variants; (2) improving sensitivity to overcome false negatives; and (3) signal readout in resource-limited settings. The subsequent part provides fundamental insights into FNA and technical tricks to successfully achieve effective COVID-19 diagnosis by using in vitro selection of FNA to overcome receptor design barriers, combining FNA with multiple DNA signal amplification strategies to improve sensitivity, and interfacing FNA with portable analyzers to overcome signal readout barriers. This review concludes with an overview of further opportunities and emerging applications for FNA-based sensors against COVID-19. Graphical abstract

show abstract

Mix-and-read, one-minute SARS-CoV-2 diagnostic assay: development of PIFE-based aptasensor

Abstract: Here, we developed a rapid and quantitative one-step severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antigen assay based on protein-induced fluorescence enhancement (PIFE) of a nucleocapsid (N) protein DNA aptamer.

Cited by 12 publications

References 33 publications

Aptamer-based biosensors for virus protein detection

Aptamer-based biosensors for virus protein detection

Aptamers for SARS‐CoV‐2: Isolation, Characterization, and Diagnostic and Therapeutic Developments

Recent advances of functional nucleic acid-based sensors for point-of-care detection of SARS-CoV-2

Contact Info

Product

Resources

About