Mitracarpus frigidus: A promising antifungal in the treatment of vulvovaginal candidiasis

Campos, Lara Melo; Melo, Lívia de Lima; Lemos, Ari S.O.; Guedes, Maria Clara Machado Resende; Silva, Thiago Pereira da; Figueiredo, Geisa Fantini de; Reis, Janildo Ludolf; Rocha, Vinícius Novaes; Melo, Rossana C. N.; Araújo, Marcelo Gonzaga Freitas; Apolônio, Ana Carolina Morais; Scio, Elita; Fabri, Rodrigo Luiz

doi:10.1016/j.indcrop.2018.07.038

Cited by 17 publications

(10 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Samples (10 μL) from each well that showed no visible growth in the MIC assay were inoculated on freshly prepared SDA plates and incubated at 35°C for 48 h. The MFC was reordered as the concentration of the scopoletin inhibiting the visible growth on a new set of agar plates ( Campos et al, 2018 ).…”

Section: Methodsmentioning

confidence: 99%

“…Fungal killing assay was performed as before (Campos et al, 2018) with some modifications. To determine the time-kill kinetics for C. tropicalis strain, freshly grown yeasts in BHI (35 • C, overnight) were standardized to 10 6 cells/mL in sterile water and inoculated in test tubes with BHI broth and different concentrations of scopoletin or fluconazole (MIC, MIC/2 and MIC/4 values).…”

Section: Fungal Killing Assaymentioning

confidence: 99%

“…Nucleotide release was evaluated as before (Khan et al, 2013;Campos et al, 2018). After 48 h incubation in BHI broth, yeast cells were washed, resuspended in 10 mM PBS (pH 7.4, ∼ 10 6 cells/mL) and incubated with scopoletin (MIC value).…”

Section: Nucleotide Leakagementioning

confidence: 99%

See 2 more Smart Citations

Antifungal Activity of the Natural Coumarin Scopoletin Against Planktonic Cells and Biofilms From a Multidrug-Resistant Candida tropicalis Strain

et al. 2020

Self Cite

View full text Add to dashboard Cite

Candida tropicalis is one the most relevant biofilm-forming fungal species increasingly associated with invasive mucosal candidiasis worldwide. The amplified antifungal resistance supports the necessity for more effective and less toxic treatment, including the use of plant-derived natural products. Scopoletin, a natural coumarin, has shown antifungal properties against plant yeast pathogens. However, the antifungal activity of this coumarin against clinically relevant fungal species such as C. tropicalis remains to be established. Here, we investigated the potential antifungal properties and mechanisms of action of scopoletin against a multidrug-resistant C. tropicalis strain (ATCC 28707). First, scopoletin was isolated by high-performance liquid chromatography from Mitracarpus frigidus, a plant species (family Rubiaceae) distributed throughout South America. Next, scopoletin was tested on C. tropicalis cultivated for 48h in both planktonic and biofilm forms. Fungal planktonic growth inhibition was analyzed by evaluating minimal inhibitory concentration (MIC), time-kill kinetics and cell density whereas the mechanisms of action were investigated with nucleotide leakage, efflux pumps and sorbitol and ergosterol bioassays. Finally, the scopoletin ability to affect C. tropicalis biofilms was evaluated through spectrophotometric and whole slide imaging approaches. In all procedures, fluconazole was used as a positive control. MIC values for scopoletin and fluconazole were 50 and 250 µg/L respectively, thus demonstrating a fungistatic activity for scopoletin. Scopoletin induced a significant decrease of C. tropicalis growth curves and cell density (91.7% reduction) compared to the growth control. Its action was related to the fungal cell wall, affecting plasma membrane sterols. When associated with fluconazole, scopoletin led to inhibition of efflux pumps at the plasma membrane. Moreover, scopoletin not only inhibited the growth rate of preformed biofilms (68.2% inhibition at MIC value) but also significantly decreased the extent of biofilms growing on the surface of coverslips, preventing the formation of elongated

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Fungal Killing Assaymentioning

confidence: 99%

See 1 more Smart Citation

Antifungal Activity of the Natural Coumarin Scopoletin Against Planktonic Cells and Biofilms From a Multidrug-Resistant Candida tropicalis Strain

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Tables 2 and 3 were used to organize the information gained from each study [11][12][13][14][15][16][17][18][19]. Table 2 displays data from combined in vivo/in vitro studies whilst Table 3 contains information from in vitro studies only .…”

Section: Data Extractionmentioning

confidence: 99%

“…The most active plants were C. sinensis with MIC values of 40 µg/mL [13]. Topical applications of 4% L. innermis show similar or better effect than clotrimazole and Mitracarpus frigidus presents antifungal activities greater than fluconazole against candidiasis [11,16]. M. alternifolia inhibited biofilm formation [15] Syzygium cumini and Jatropha curcas, which contained a high amount of phenols and flavonoids are responsible for the inhibitory effect against C. albicans [17,19].…”

Section: Herbal Interventions In Vivomentioning

confidence: 99%

Herbal Extracts with Antifungal Activity against Candida albicans: A Systematic Review

Hsu

Sheth

Veses

2021

MRMC

View full text Add to dashboard Cite

Background: In the era of antimicrobial resistance, fungal pathogens are not an exception. Several strategies, including antimicrobial stewardship programs and high throughput screening of new drugs are being implemented. Several recent studies have demonstrated effectiveness of plant compounds with antifungal activity. Objective: In this systematic review we examine the use of natural compounds as a possible avenue to fight fungal infections produced by Candida albicans, the most common human fungal pathogen. Method: Electronic literature searches were conducted through PubMed/MEDLINE, Cochrane, and Science Direct limited to the 5 years. A total of 131 articles were included with 186 plants extracts evaluated. Results: Although the majority of the natural extracts exhibited antifungal activities against C. albicans (both in vivo and in vitro), the strongest antifungal activity was obtained from Lawsonia inermis, Pelargonium graveolens, Camellia sinensis, Mentha piperita, and Citrus latifolia. Conclusion: The main components with proven antifungal activities were phenolic compounds such as gallic acid, thymol, and flavonoids (specially catechin), polyphenols such as tannins, terpenoids and saponins. The incorporation of nanotechnology enhances greatly the antifungal properties of these natural compounds. Further research is needed to fully characterize the composition of all herbal extracts with antifungal activity as well as the mechanisms of action of the active compounds.

show abstract

Isolation and Chemical Characterization of Antifungal, Antioxidant, and Anti‐Inflammatory Compounds from Centrosema coriaceum using GC/MS, UFLC‐QTOF‐MS, and FACE

Lemos

Campos

Freitas

et al. 2022

Chemistry & Biodiversity

Self Cite

View full text Add to dashboard Cite

In recent years, natural products with biological activities have been increasingly researched. The elucidation of phytoconstituents is necessary for the development of drugs as a natural alternative for the treatment of various diseases. The work aimed to evaluate in vitro and in silico bioactivities of hexane (CCHE) and methanol (CCME) fractions of ethanolic extract from Centrosema coriaceum Benth (Fabaceae) leaves and elucidate their phytoconstituents. CCHE and CCME showed antifungal activity for Candida glabrata (MIC of 1000 μg/mL) with fungistatic effect and action in cell envelope by sorbitol and ergosterol assays. CCHE and CCME presented promising antioxidant activity against the DPPH radical with IC 50 of 13.61 � 0.50 and 6.31 � 0.40 μg/mL, respectively, and relative antioxidant activity (RAA%) of 45.77 � 3.61/ 28.53 � 2.25 % for CCHE and 82.18 � 2.25/ 51.99 � 3.23 % for CCME when compared to rutin and quercetin, respectively. Moreover, these fractions demonstrated promising results for the inhibition of lipid peroxidation by β-carotene/linoleic acid assay. For antiinflammatory and cytotoxicity activities, CCHE and CCME significantly inhibited the production of nitric oxide and TNF-α, without toxicity on murine intraperitoneal macrophages, respectively. Esters, alkanes, steroids, tocopherols, and terpenes were identified in CCHE by GC/MS. Flavonoids, phenolic acids, and disaccharides were detected in CCME by UFLC-QTOF-MS and FACE. Furthermore, rutin was purified from CCME. In silico predictions evidenced that compounds present in both fractions have high affinity to the fungal membrane besides antioxidant and anti-inflammatory activities. Based on these observations, CCHE and CCME have a noteworthy potential for the design of novel antifungal and anti-inflammatory agents that should be explored in future studies.

show abstract

Mitracarpus frigidus: A promising antifungal in the treatment of vulvovaginal candidiasis

Cited by 17 publications

References 44 publications

Antifungal Activity of the Natural Coumarin Scopoletin Against Planktonic Cells and Biofilms From a Multidrug-Resistant Candida tropicalis Strain

Antifungal Activity of the Natural Coumarin Scopoletin Against Planktonic Cells and Biofilms From a Multidrug-Resistant Candida tropicalis Strain

Herbal Extracts with Antifungal Activity against Candida albicans: A Systematic Review

Isolation and Chemical Characterization of Antifungal, Antioxidant, and Anti‐Inflammatory Compounds from Centrosema coriaceum using GC/MS, UFLC‐QTOF‐MS, and FACE

Contact Info

Product

Resources

About