Mitotic WNT: aligning chromosomes through KIF2A

Bufe, Anja; Acebrón, Sergio P.

doi:10.1080/23723556.2021.2011564

Cited by 2 publications

(3 citation statements)

References 9 publications

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“…[3][4][5] Hence, breast cancer is still a hot topic in clinical research, and actively investigating potential biomarkers for monitoring progression in breast cancer patients is vital. [6][7][8] Kinesin family member 2A (KIF2A) is a member of the kinesin-13 family that is a conserved class of microtubule-dependent motor proteins, 9,10 which participants in the processes of tumor growth and metastasis in cancers (including breast cancer, 11 gastric cancer, 12 and squamous cell carcinoma of the oral tongue [SCCOT]). 13 Based on existing evidence from Protein-Protein Interaction (PPI) network by String Database (https://strin g-db.org/), 14 KIF2A has a multiple regulation relationship with nuclear division cycle 80 (NDC80), cyclindependent kinase 1 (CDK1), and cyclin B1 (CCNB1).…”

Section: Introductionmentioning

confidence: 99%

Expression of KIF2A, NDC80, CDK1, and CCNB1 in breast cancer patients: Their interaction and linkage with tumor features and prognosis

Xie

Jiang

2022

Clinical Laboratory Analysis

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Background Kinesin family member 2A (KIF2A), nuclear division cycle 80 (NDC80), cyclin‐dependent kinase 1 (CDK1), and cyclin B1 (CCNB1) exhibit a complex interrelation, which promote cancer progression via multiple ways, whereas their interaction and clinical implications in breast cancer are obscure. Hence, this study aimed to evaluate the correlation among KIF2A, NDC80, CDK1, CCNB1, and their linkage with clinicopathological features and prognosis in breast cancer patients. Methods 195 breast cancer patients underwent surgical resection were analyzed. KIF2A, NDC80, CDK1, and CCNB1 expressions were determined by immunohistochemical (IHC) assay and scored by a semiquantitative IHC score or positive cell percentage. Results KIF2A expression positively associated with NDC80, CDK1, and CCNB1 expressions (all p < 0.01). In terms of tumor features: KIF2A high expression linked with increased T stage ( p = 0.011), N stage ( p = 0.014), and TNM stage ( p = 0.009) but not tumor differentiation ( p = 0.651). NDC80 high expression only related to higher N stage ( p = 0.010); CDK1 high expression only connected with elevated N stage ( p = 0.035) and TNM stage ( p = 0.023). In aspect of prognosis, high expression of KIF2A was correlated with worse disease‐free survival (DFS) ( p = 0.031), while NDC80 high ( p = 0.329), CDK1 high ( p = 0.276), and CCNB1 positive ( p = 0.063) expressions only showed trends to link with poor DFS (without statistical significance). Furthermore, high expression of KIF2A ( p = 0.063), NDC80 ( p = 0.939), CDK1 ( p = 0.413) and positive expression of CCNB1 ( p = 0.296) did not relate to overall survival. Conclusion KIF2A correlates with NDC80, CDK1, CCNB1, and may link with advanced tumor stages and poor prognosis in breast cancer patients.

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Section: Introductionmentioning

confidence: 99%

Expression of KIF2A, NDC80, CDK1, and CCNB1 in breast cancer patients: Their interaction and linkage with tumor features and prognosis

Xie

Jiang

2022

Clinical Laboratory Analysis

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“…As we mentioned before, the MRN complex [ 34 ] and DVL ligand [ 35 , 36 , 37 ] are responsible for KIF2A-PLK1 complex recruitment during mitosis or cilia formation. In this case, are MRN and DVL also involved in the recruitment of the KIF2A/PLK1 complex during the regulation of the PI3K/AKT pathway?…”

Section: Discussion and Perspectivesmentioning

confidence: 99%

“…Their interaction depends on the Mre11-Rad50-NbS1 (MRN) complex [ 34 ]. Also, Wnt ligands can induce LRP to the DVL platform, recruit KIF2A and PLK1 to DVL, and form complexes with DVL, LRP, and the Wnt receptor Frizzled, promoting KIF2A activation and spindle pole localization [ 35 , 36 ]. In addition, KIF2A, PLK1, and DVL complexes are also involved in the formation of cilia [ 37 ].…”

Section: Introductionmentioning

confidence: 99%

KIF2A Upregulates PI3K/AKT Signaling through Polo-like Kinase 1 (PLK1) to Affect the Proliferation and Apoptosis Levels of Eriocheir sinensis Spermatogenic Cells

Zhao,

Liu,

Tan

et al. 2024

Biology

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E. sinensis is an animal model for studying the reproduction and development of crustaceans. In this study, we knocked down the Es-Kif2a gene by injecting dsRNA into E. sinensis and inhibited Es-Plk1 gene expression by injecting PLK1 inhibitor BI6727 into E. sinensis. Then, the cell proliferation level, apoptosis level, and PI3K/AKT signaling expression level were detected. Our results showed that the proliferation level of spermatogenic cells decreased, while the apoptosis level increased after Es-Kif2a knockdown or Es-Plk1 inhibition. In order to verify whether these changes are caused by regulating the PI3K/AKT pathway, we detected the expression of PI3K and AKT proteins after Es-Kif2a knockdown or Es-Plk1 inhibition. Western Blot showed that in both the Es-Kif2a knockdown group and the Es-Plk1 inhibition group, the expression of PI3K and AKT proteins decreased. In addition, immunofluorescence showed that Es-KIF2A and Es-PLK1 proteins were co-localized during E. sinensis spermatogenesis. To further explore the upstream and downstream relationship between Es-KIF2A and Es-PLK1, we detected the expression level of Es-PLK1 after Es-Kif2a knockdown as well as the expression level of Es-KIF2A after Es-Plk1 inhibition. Western Blot showed that the expression of Es-PLK1 decreased after Es-Kif2a knockdown, while there was no significant change of Es-KIF2A after Es-Plk1 inhibition, indicating that Es-PLK1 may be a downstream factor of Es-KIF2A. Taken together, these results suggest that Es-KIF2A upregulates the PI3K/AKT signaling pathway through Es-PLK1 during the spermatogenesis of E. sinensis, thereby affecting the proliferation and apoptosis levels of spermatogenic cells.

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Mitotic WNT: aligning chromosomes through KIF2A

Cited by 2 publications

References 9 publications

Expression of KIF2A, NDC80, CDK1, and CCNB1 in breast cancer patients: Their interaction and linkage with tumor features and prognosis

Expression of KIF2A, NDC80, CDK1, and CCNB1 in breast cancer patients: Their interaction and linkage with tumor features and prognosis

KIF2A Upregulates PI3K/AKT Signaling through Polo-like Kinase 1 (PLK1) to Affect the Proliferation and Apoptosis Levels of Eriocheir sinensis Spermatogenic Cells

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