Mitotic DNA damage targets the Aurora A/TPX2 complex

Bhatia, Payal; Menigatti, Mirco; Brocard, Michèle; Morley, Simon; Ferrari, Stefano

doi:10.4161/cc.9.22.13665

Cited by 6 publications

(10 citation statements)

References 44 publications

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“…DNA is normally regulated by many proteins that can identify the damage type and execute DNA repair [8]. With respect to such repairs, cells activate the signal mechanism to arrest the cell cycle [17].…”

Section: Discussionmentioning

confidence: 99%

“…Krystyniak et al [18] demonstrated that DNA damage produced the arresting of the G2 transition in the cell cycle and accumulated the Aurora-A protein. Bhatia et al [8] reported that mitotic DNA damage targeted Aurora-A. Further, a coincidence of the expression of elevated Aurora-A and the suspension of a DNA damage-induced cell death response was observed in the study conducted by Katayama et al [19].…”

Section: Discussionmentioning

confidence: 99%

“…Hsieh et al [7] reported that oxidative stress causes DNA damage in diabetic rats. Recently, Bhatia et al [8] reported that DNA damage targets Aurora-A. Although Aurora-A is known to have oncogenic properties, thus far, no studies on the expression of Aurora-A in human diabetic skin tissue have been reported.…”

Section: Introductionmentioning

confidence: 99%

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Elevated Aurora Kinase A Protein Expression in Diabetic Skin Tissue

Cho

Kim

et al. 2014

Arch Plast Surg

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BackgroundAurora kinase A (Aurora-A) plays an important role in the regulation of mitosis and cytokinesis. Dysregulated Aurora-A leads to mitotic faults and results in pathological conditions. No studies on Aurora-A expression in human diabetic skin tissue have been reported. In light of this, we explored the expression of Aurora-A in human diabetic skin tissue.MethodsAurora-A protein was evaluated by western blotting in 6 human diabetic skin tissue and 6 normal skin specimens.ResultsIncreased expression of Aurora-A protein was detected in all diabetic skin tissue samples in both western blot analysis and immunohistochemical staining. However, in the case of the normal skin tissue, no bands of Aurora-A protein were detected in either the western blotting analysis or the immunohistochemical staining.ConclusionsThus far, there have been no studies on the expression of Aurora-A in diabetic skin tissue. However, we believe that oxidative DNA damage related to the expression of Aurora-A protein and Aurora-A could be involved inhuman diabetic skin tissue.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Elevated Aurora Kinase A Protein Expression in Diabetic Skin Tissue

Cho

Kim

et al. 2014

Arch Plast Surg

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“…This centrosomal function of intracellular RHAMM is required for cell division fidelity in vascular response to injury, mitotic spindle integrity, progression through G2M, and basal-apical polarity of breast epithelial cells [57, 59, 197–199]. Nuclear RHAMM may play a further role in sequestering TPX2 [49] to these compartments to prevent premature changes in microtubules/mitotic spindle assembly and to facilitate repair of DNA aberrations caused, for example, by ionizing radiation [200]. In addition, RHAMM together with CD44 or TGF β R1, and possibly intracellular HA, may directly affect the transcription of genes controlling cell migration and proliferation [170, 190, 201] (e.g., PAI-1, MMP-9, Figure 4).…”

Section: Rhamm Signalingmentioning

confidence: 99%

Hyaluronan and RHAMM in Wound Repair and the “Cancerization” of Stromal Tissues

Tölg

McCarthy²,

Yazdani

et al. 2014

BioMed Research International

111

144

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Tumors and wounds share many similarities including loss of tissue architecture, cell polarity and cell differentiation, aberrant extracellular matrix (ECM) remodeling (Ballard et al., 2006) increased inflammation, angiogenesis, and elevated cell migration and proliferation. Whereas these changes are transient in repairing wounds, tumors do not regain tissue architecture but rather their continued progression is fueled in part by loss of normal tissue structure. As a result tumors are often described as wounds that do not heal. The ECM component hyaluronan (HA) and its receptor RHAMM have both been implicated in wound repair and tumor progression. This review highlights the similarities and differences in their roles during these processes and proposes that RHAMM-regulated wound repair functions may contribute to “cancerization” of the tumor microenvironment.

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“…These questions remain to be addressed in the future. These kinase activator proteins [22,23] may also have a broad spectrum of biological significance, which deserves further attention. Aurora kinases and many other kinase family members that require coactivator proteins are known to be deregulated in cancers and other pathological states.…”

Section: Discussionmentioning

confidence: 99%

Multifunctional human transcriptional coactivator protein PC4 is a substrate of Aurora kinases and activates the Aurora enzymes

et al. 2016

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Positive coactivator 4 (PC4), a human transcriptional coactivator, is involved in diverse processes like chromatin organization and transcription regulation. It is hyperphosphorylated during mitosis, with unknown significance. For the first time, we demonstrate the function of PC4 outside the nucleus upon nuclear envelope breakdown. A fraction of PC4 associates with Aurora A and Aurora B and undergoes phosphorylation, following which PC4 activates both Aurora A and B to sustain optimal kinase activity to maintain the phosphorylation gradient for the proper functioning of the mitotic machinery. This mitotic role is evident in PC4 knockdown cells where the defects are rescued only by the catalytically active Aurora kinases, but not the kinase-dead mutants. Similarly, the PC4 phosphodeficient mutant failed to rescue such defects. Hence, our observations establish a novel mitotic function of PC4 that might be dependent on Aurora kinase-mediated phosphorylation.

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Mitotic DNA damage targets the Aurora A/TPX2 complex

Cited by 6 publications

References 44 publications

Elevated Aurora Kinase A Protein Expression in Diabetic Skin Tissue

Elevated Aurora Kinase A Protein Expression in Diabetic Skin Tissue

Hyaluronan and RHAMM in Wound Repair and the “Cancerization” of Stromal Tissues

Multifunctional human transcriptional coactivator protein PC4 is a substrate of Aurora kinases and activates the Aurora enzymes

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