Mitotic abnormalities and micronuclei inducing potentials of colchicine and leaf aqueous extracts ofClerodendrum viscosumVent. inAllium ceparoot apical meristem cells

Cell Cycle Delay, Pro-metaphase Arrest and C-metaphase Inducing Effects of Petroleum Ether Fraction of Leaf Aqueous Extract ofClerodendrum viscosumVent

“…The different extracts of Clerodendrum viscosum have anti-septic, anti-helminthic, antiinflammatory, anti-leprosy, anti-pyretic, anti-tumor, anti-bacterial, antiproliferative, metaphase arresting, apoptosis-inducing, antifeedant activities, etc. [51,52,53,17,54,31,34,33]. This study is in continuous with the previous investigation that indicated antiproliferative, metaphase arresting, mitotic abnormality, and micronuclei inducing potentials of leaf aqueous extract of C.…”

“…In our previous study, we have reported that treatment with leaf aqueous extract of C. viscosum (LAECV) on root apical meristem cells of wheat and onion gave an increased metaphase frequency along with a reduction in mitotic index, antiproliferative, and apoptosis-inducing effects [31]. The metaphase arrest and cell cycle delay inducing effects were somewhat comparable to Colchicine’s actions [32, 33, 34].…”

“…Colchicine treatment to onion root apical meristem cells, the root growth was inhibited, roots were swelled, and chromosomes were condensed and arranged haphazardly along with an increased frequency in metaphase [34]. In another comparative study, treatment of colchicine and LAECV on Allium cepa root tip cells reveals their similar cytogenetic effects concerning an increased frequency in mitotic abnormalities and micronucleus [33]. The metaphase arrest and cell cycle delay inducing effects of LAECV raised the key question about its active principle(s) [34].…”

Deciphering colchicine like actions of clerodin in terms of microtubule destabilization based mitotic abnormalities, G2/M-phase arrest, and plant polyploidy

“…(Family: Lamiaceae) is used as an antidote of snakebite, anthelmintic (Saroj 2016), analgesic and anticonvulsant, antioxidant, antimicrobial, hepatoprotective, wound healing and antidiarrheal (Bhattacharjee et al 2011), hypoglycemic (Hossain et al 2014, Chandrashekar et al 2012, cytotoxic, anthelmintic (Rahman et al 2013), negative impact on the growth and germination of weeds in an agroecosystem (Devi et al 2013, Qasem et al 2001, insect repellent and insecticidal activity (Muh et al 2014). Recently, we have shown a colchicine-like antiproliferative and metaphase arresting potentials of the aqueous extract of C.viscosum leaf (Ray et al 2013, Kundu et al 2016. All these results indicate that the clerodane diterpenoids are the major bioactive components of C. viscosum (Ray et al 2013, Kumar et al 2018, Abbaszadeh et al 2012.…”

Vitamin C may exert variable effects on viability and proliferation of HeLa cells exhibiting high and low chromosomal instability

“…To induce a higher degree of CIN, we treated the HeLa cells with a high dose of colchicine. 8 The 2 types of cells, when treated with vitamin C, showed paradoxical results. Vitamin C was found to enhance CIN in CIN lo cells, at the same time decreasing their viability and proliferation.…”