Mitosis of radial glial cells in the optic tectum of adult goldfish

Ja, Stevenson; Yoon, MG

doi:10.1523/jneurosci.01-08-00862.1981

Cited by 60 publications

(24 citation statements)

References 12 publications

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“…The resultant slides of HVc were osmium-fixed, dehydrated, and embedded into Araldite. Adjacent 1-gm semithin and 100-to 150-nm thin sections were then cut and mounted onto slides or 200-mesh copper grids, respectively (9). The I-nm sections were dipped into Kodak NTB-3 emulsion, exposed for 6-7 wk at 40C, and then developed as above and counterstained with methylene blue/azure.…”

Section: Methodsmentioning

confidence: 99%

Neuronal production, migration, and differentiation in a vocal control nucleus of the adult female canary brain.

Goldman¹,

Nottebohm²

1983

Proc. Natl. Acad. Sci. U.S.A.

855

545

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The vocal control nucleus designated HVc (hyperstriatum ventrale, pars caudalis) of adult female canaries expands in response to systemic testosterone administration, which also induces the females to sing in a male-like manner. We became interested in the possibility of neurogenesis as a potential basis for this phenomenon. Intact adult female canaries were injected with [3H]thymidine over a 2-day period. Some birds were given testosterone implants at various times before thymidine. The birds were sacrificed 5 wk after hormone implantation, and their brains were processed for autoradiography. In parallel control experiments, some birds were given implants of cholesterol instead of testosterone. All birds showed considerable numbers of labeled neurons, glia, endothelia, and ventricular zone cells in and around HVc. Ultrastructural analysis confirmed the identity of these labeled neurons. Cholesterol-and testosterone-treated birds had similar neuronal labeling indices, which ranged from 1.8% to 4.0% in HVc. Thus, neurogenesis occurred in these adults independently of exogenous hormone treatment. Conversely, both glial and endothelial proliferation rates were markedly stimulated by exogenous testosterone treatment. We determined the origin of the thymidine-incorporating neurons by sacrificing two thymidine-treated females soon after their thymidine injections, precluding any significant migration of newly labeled cells. Analysis of these brains revealed no cells of neuronal morphology present in HVc but a very heavily labeled ventricular zone overlying HVc. We conclude that neuronal precursors exist in the HVc ventricular zone that incorporate tritiated thymidine during the S phase preceding their mitosis; after division these cells migrate into, and to some extent beyond, HVc. This ventricular zone neurogenesis seems to be a normally occurring phenomenon in intact adult female canaries.The primary telencephalic song-control nucleus, HVc (hyperstriatum ventrale, pars caudalis) (1, 2), of adult female canaries doubles in size in response to androgen treatment as these birds develop male-like song (3). We became interested in the possibility that addition of neurons might contribute to this increase in HVc volume. We report here that new neurons are added at relatively high rates to nucleus HVc of adult intact female canaries. This occurs as ventricular zone stem cells proliferate, and then some of the daughter cells migrate into HVc and differentiate into young neurons. This adult neurogenesis occurs independently of exogenous testosterone treatment; however, both glial and endothelial proliferation can be stimulated by testosterone administration. MATERIALS AND METHODSOn day 0 of this study (June 10, 1981) 18 intact, 1-yr-old female canaries were implanted with Silastic tubes (4) containing either testosterone (14 birds), cholesterol (3 birds), or nothing (1 bird). For successive 2-day periods beginning with day 0, individual birds received intramuscular (M. pectoralis) injections of [3H]thymidine ([me...

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Section: Methodsmentioning

confidence: 99%

Neuronal production, migration, and differentiation in a vocal control nucleus of the adult female canary brain.

Goldman¹,

Nottebohm²

1983

Proc. Natl. Acad. Sci. U.S.A.

855

545

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“…Using electron microscopic radioautography, Reznikov et al (1981) concluded that postnatal glial proliferation is independent from neurogenesis and that astroglia are capable of proliferation even in advanced stages of differentiation. Sturrock (1981) demonstrated with the electron microscope that oligodendrocytes which have commenced myelination are capable of undergoing mitosis without losing contact with their myelin sheaths, and Stevenson and Yoon (1981) have identified mitotic radial glial cells.…”

Section: Extraventricularmentioning

confidence: 99%

“…Although recent studies have reported that advanced differentiated neuroglia may divide (Cavanaugh, 1970;Sturrock, 1975Sturrock, , 1976Latov et al, 1979;Kaplan and Hinds, 1980;Sturrock and McRae, 1980;Reznikov et al, 1981;Sturrock, 1981), there have previously been conceptual obstacles that differentiated nerve cells or neuroblasts may divide. There are less strong objections to the division of other CNS cell types with processes and junctions during mitoses (Kaplan, 1980;Sturrock and McRae, 1980;Stevenson and Yoon, 1981;Sturrock, 1981). In fact, Gershon et al (1981) reported mitotic enteric neuroblasts with phenotypic characteristics of mature neurons.…”

mentioning

confidence: 99%

Mitotic neuroblasts in the 9-day-old and 11-month-old rodent hippocampus

Kaplan¹,

Bell²

1984

J. Neurosci.

419

215

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Ultrastructural identification of mitotic neuronal precursors beneath the basal hippocampal granule cell layer was made using electron micrographs of [3H]thymidine-labeled cells. Ultrathin sections were obtained by a method that allows serial thin sectioning of reembedded sections previously prepared for light microscopic radioautography.The electron microscopic observations reported in this study reveal: (I) that a steady rate of granule cell neurogenesis occurs during the first year of a rodent's life; (2) that newly formed granule neurons in the dentate gyrus of the newborn mouse and adult rat are a result of neuroblast division; and (3) two distinct classes of mitotic cells can be identified during the peak period of postnatal neurogenesis-those with synapses on their cell bodies and processes and those with no synapses or processes.

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“…In previous studies, mitotic radial glial cells were observed in the lower SPV zone of goldfish during the regeneration period of the optic nerve after surgical crush (Stevenson and Yoon, 1981). However, the boundary between periventricular neurons and glial cells was unclear.…”

Section: Radial Glial Cells In the Optic Tectummentioning

confidence: 93%

Retinotectal transmission in the optic tectum of rainbow trout

Kinoshita

Fukaya

Tojima

et al. 2005

J of Comparative Neurology

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Retinotectal transmission has not yet been well characterized at the cellular level in the optic tectum. To address this issue, we used a teleost, the rainbow trout, and characterized periventricular neurons as postsynaptic cells expected to receive the retinotectal inputs to the optic tectum. The somata of periventricular neurons are localized in the upper zone of the stratum periventriculare (SPV), whereas the lower zone of the SPV comprises the cell body layer of radial glial cells. Ca2+ imaging identified functional ionotropic glutamate receptors in periventricular neurons. We also cloned cDNAs encoding the NR1 subunit of N-methyl-D-aspartic acid (NMDA) receptors and the GluR2 subunit of (+/-)-alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionic acid (AMPA) receptors, and detected their mRNAs in periventricular neurons by in situ hybridization. The presence of the receptor subunit proteins was also confirmed in the dendrites of periventricular neurons by immunoblotting and immunohistochemistry. On the other hand, radial glial cells in the lower zone of the SPV did not respond to glutamate applications, and mRNA and immunoreactivities of ionotropic glutamate receptors were not detected in glial cells. The present findings suggest that glutamatergic transmission at synapses between retinotectal afferents and periventricular neurons is mediated by the functional NMDA and AMPA receptors.

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Mitosis of radial glial cells in the optic tectum of adult goldfish

Cited by 60 publications

References 12 publications

Neuronal production, migration, and differentiation in a vocal control nucleus of the adult female canary brain.

Neuronal production, migration, and differentiation in a vocal control nucleus of the adult female canary brain.

Mitotic neuroblasts in the 9-day-old and 11-month-old rodent hippocampus

Retinotectal transmission in the optic tectum of rainbow trout

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