Mitomycin C-induced cell cycle arrest enhances 5-aminolevulinic acid-based photodynamic therapy for bladder cancer

Nakayama, Taku; Nozawa, Naoko; Kawada, Chiaki; Yamamoto, Shinkuro; Ishii, Takuya; Ishizuka, Masahiro; Namikawa, Tsutomu; Ogura, Shun‐ichiro; Hanazaki, Kazuhiro; Inoue, Keiji; Karashima, Takashi

doi:10.1016/j.pdpdt.2020.101893

Cited by 19 publications

(21 citation statements)

References 27 publications

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“…An unpaired twotailed t-test was used to test the signi cance of differences between groups. The data were expressed as the mean ± SE of at least three independent experiments [6].…”

Section: Discussionmentioning

confidence: 99%

“…The human prostate cancer cell line PC-3 (provided by Dr. Inoue, Kochi University, Kochi, Japan) was maintained in DMEM supplemented with 10% (v/v) FBS and 1% (v/v) ABAM. Cells were maintained in a 5% CO 2 atmosphere at 37°C [6].…”

Section: Cells and Cell Culturesmentioning

confidence: 99%

“…Another report insisted that ABCB6 expression was critical for PpIX accumulation in cancer cells after ALA administration [10]. Altogether, the results of these previous studies suggest that PEPT1, ABCB6, and ABCG2 are critically involved in porphyrin metabolism and regulate PpIX accumulation [2,6].…”

mentioning

confidence: 92%

“…In normal cells, administrated ALA will be synthesized to be protoporphyrin IX (PpIX) and almost all will be inserted iron ions to be heme. However, in tumor cells, previous studies revealed PpIX accumulation as well as heme generation [2][3][4][5][6]. PpIX is a uorescent substance which has maximum excitation at 405 nm and emission at 635 nm, respectively.…”

mentioning

confidence: 99%

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Enhanced Lipid Metabolism Induces the Sensitivity of Dormant Cancer Cells to 5-aminolevulinic acid-based Photodynamic Therapy

Nakayama

Sano

Oshimo

et al. 2021

Preprint

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Cancer can develop into a recurrent metastatic disease with latency periods of years to decades. Dormant cancer cells, which represent a major cause of recurrent cancer, are relatively insensitive to most chemotherapeutic drugs and radiation. We previously demonstrated that cancer cells exhibited dormancy in a cell density-dependent manner. Dormant cancer cells exhibited increased porphyrin metabolism and sensitivity to 5-aminolevulinic acid-based photodynamic therapy (ALA-PDT). However, the metabolic changes in dormant cancer cells or the factors that enhance porphyrin metabolism have not been fully clarified. In this study, we revealed that lipid metabolism was increased in dormant cancer cells, leading to ALA-PDT sensitivity. We performed microarray analysis in non-dormant and dormant cancer cells and revealed that lipid metabolism was remarkably enhanced in dormant cancer cells. In addition, triacsin C, a potent inhibitor of acyl-CoA synthetases (ACSs), reduced protoporphyrin IX (PpIX) accumulation and decreased ALA-PDT sensitivity. We demonstrated that lipid metabolism including ACS expression was positively associated with PpIX accumulation. This research suggested that the enhancement of lipid metabolism in cancer cells induces PpIX accumulation and ALA-PDT sensitivity.

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“…An unpaired twotailed t-test was used to test the signi cance of differences between groups. The data were expressed as the mean ± SE of at least three independent experiments [6].…”

Section: Discussionmentioning

confidence: 99%

Section: Cells and Cell Culturesmentioning

confidence: 99%

mentioning

confidence: 92%

mentioning

confidence: 99%

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Enhanced Lipid Metabolism Induces the Sensitivity of Dormant Cancer Cells to 5-aminolevulinic acid-based Photodynamic Therapy

Nakayama

Sano

Oshimo

et al. 2021

Preprint

Self Cite

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“…Various metals are inserted into the structure of porphyrins, and these metal porphyrins have several functions in vivo. For example, magnesium ions inserted in porphyrins are important constituents of chlorophyll, and iron ions inserted in porphyrins are important constituents of heme, which functions as an active site for various enzymes such as CYP450, catalase, and mitochondrial electron transport chain complexes 1 , 2 . Protoporphyrin IX (PpIX) is a fluorophore with a maximum excitation wavelength of about 405 nm and a maximum emission wavelength of about 635 nm; metal-free porphyrins, including PpIX, function as photosensitizers.…”

Section: Introductionmentioning

confidence: 99%

Enhanced lipid metabolism induces the sensitivity of dormant cancer cells to 5-aminolevulinic acid-based photodynamic therapy

Nakayama

Sano²,

Oshimo

et al. 2021

Sci Rep

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Gemcitabine‐Loaded Injectable Hydrogel for Localized Breast Cancer Immunotherapy

Barough,

Seyfoori,

Askari

et al. 2024

Adv Funct Materials

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Injectable hydrogels for cancer immunotherapy are effective for both active and passive approaches. Tumor‐infiltrating lymphocyte (TIL) immunoshaping can change the tumor microenvironment to favor tumor cell elimination. The primary objective of immunoshaping is to reduce regulatory T‐cells (Tregs), which can enhance the effectiveness of ex vivo immune cell therapy in solid tumors. A shear‐thinning injectable hydrogel that consists of gelatin and Laponite (Gel‐Lap) is used in this study. By optimizing the formulation, their immunotherapeutic and anti‐tumor properties are examined. Gemcitabine (GEM), an anti‐metabolite cancer chemotherapy agent, is loaded into a Gel‐Lap hydrogel (immunogel). The study compares the effects of immunogel on 4T1 inoculated breast cancer animal models. Results show that immunogel increases survival rates and significantly inhibits metastasis. The Treg cell population reduction is observed up to 70% in TILs and splenocyte population in line with CD8+ T‐cells population increment in inguinal lymph nodes near the tumor region; the CD8+ T‐cells function may be mediated through overexpression of eomesodermin (EOMES) as cytotoxic T lymphocyte (CTL) activation transcription factor. The human 3D cell culture model confirmed results in animal data demonstrating T‐cell migration through the hydrogel and anticancer efficacy. Local delivery of GEM using our silicate‐based hydrogel holds promise for editing tumor microenvironment in favor of systemic immune responses.

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Mitomycin C-induced cell cycle arrest enhances 5-aminolevulinic acid-based photodynamic therapy for bladder cancer

Cited by 19 publications

References 27 publications

Enhanced Lipid Metabolism Induces the Sensitivity of Dormant Cancer Cells to 5-aminolevulinic acid-based Photodynamic Therapy

Enhanced Lipid Metabolism Induces the Sensitivity of Dormant Cancer Cells to 5-aminolevulinic acid-based Photodynamic Therapy

Enhanced lipid metabolism induces the sensitivity of dormant cancer cells to 5-aminolevulinic acid-based photodynamic therapy

Gemcitabine‐Loaded Injectable Hydrogel for Localized Breast Cancer Immunotherapy

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