Mitomycin C effects on cell cycle progression, including inhibition of very late prophase, as seen in living neuroblasts of Chortophaga viridifasciata, with some observations on mitomycin C purity☆

Gaulden, Mary Esther; Ferguson, Martha J.; Seibert, G. Burton; Proctor, Bertha L.

doi:10.1016/0027-5107(85)90007-7

Cited by 8 publications

(4 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a result of this, the DNA of each cell gathers thousands of lesions every day. These accumulated lessions or extensive damage can cause various diseases or abnormalities such as cancer [2], tumor development, arrest cell cycle progression [3] .This DNA damaged should be removed so that the cell function and proliferate normally [1,4]. This damage essential to be repaired to allow polymerases (RNA and DNA) to precisely read and duplicate the information in the genome.…”

Section: Introductionmentioning

confidence: 99%

Evaluation the Susceptibility of Metformin Drug and Pollen grain of Typha domengensis (Typha) on Prevention of Damage in octa4 gene Which Induced by Mitomycin c in Some Organs in Male Albino Rats

Alobaidy¹,

Jashammy²

2019

DJM

View full text Add to dashboard Cite

Background: Living organisms are permanently exposed to internal and external factors that damage DNA. This damage, if not repaired, can lead to the mutation which cause verius diseases and cellular death. Therefore,the use of many plants or their products has been adopted as aprotective material against genetic toxicityof certain drugs. Objective:Examined the susceptibility of both metformin and pollen grain solution to the prevention of damage in octa4 gene and mitomycin C, In order to be adopted as materials available and cheap to prevent damage to genetic material and the result of the use of human drugs. Patients and Methods: was used 25 white males rats for 4 weeks obtained from the College of Veterinary Medicine / University of Baghdad and ages ranging from (10-12) weeks. The rats were divided into 5 groups. the control group was drip with distilled water as well as injected The second group was treated with mitomycin (0.001ml) was injected subcutaneously weekly for 30 days.while the third group was orally injected with metformin with a concentration of 500 mg / kg with mitomycin , while the fourth group was treated with mitomycin and water solution for pollen at 0.1 g / mL per animal. while the fifth group included rats treated with mitomycin with metformin and water solution for pollen. The animals were anesthetized and the kidney and liver were removed. The DNA extraction process was investigated and the presence of the octa4 gene was investigated using a special technique and PCR technique.The tissues like liver, kidney and testies were collected for study the expression of Oct 4 gene. Results: The C. papyrus pollon extract along with metformin is effective against mitomicine C induced animals. Oct 4 it has been protected from mitomycin damage in both the kidney and liver tissue compared to the testis of the animals treated with C. papyrus pollon extract and metformin. C. papyrus pollen grain producing an effective and safe product, and further research is required in addition to the aggregate value to the native plants. Conclusion: In the present study, C. papyrus pollon extract along with metformin is effective against mitomicine C induced animals. Oct 4 expression was significantly higher in the kidney and liver tissue of the animals treated with C. papyrus pollon extract and metformin. The result of the present study clearly indicates that C. papyrus reduces blood

show abstract

Section: Introductionmentioning

confidence: 99%

Evaluation the Susceptibility of Metformin Drug and Pollen grain of Typha domengensis (Typha) on Prevention of Damage in octa4 gene Which Induced by Mitomycin c in Some Organs in Male Albino Rats

Alobaidy¹,

Jashammy²

2019

DJM

View full text Add to dashboard Cite

show abstract

“…In vivo, the reversible inhibition of mitosis in very late prophase (Ferguson et al, 1985;Gaulden et al, 1985) corresponding to the reversible inhibition of tubulin assembly in vitro might result either from an interaction of unactivated MMC with the mitotic spindle or from an interaction of MMC with the nuclear envelope leading to a disconnection of centromeres with the nuclear envelope. The reversibility of this in vivo effect might be related to the reductive metabolism of MMC resulting in a timedependent decrease in the concentration of unactivated MMC.…”

Section: Genotoxic Effects In Yeastmentioning

confidence: 99%

“…In neuroblasts of the grasshopper Chortophaga viridifasciata they observed a pronounced retardation of very late prophase which led to loss of chromosome orientation within the nucleus. This effect on mitosis is reversible and is expressed as an extended duration of very late prophase in relation to MMC concentration and time of exposure (Gaulden et al, 1985). This reversible inhibition of mitosis could be related to an interaction of MMC with a non-DNA target as, for example, the spindle.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Reversible inhibition of mammalian tubulin assembly in vitro and effects in Saccharomyces cerevisiae D61.M by mitomycin C

1989

View full text Add to dashboard Cite

Chromosome fragments and other abnormalities induced by mitomycin C in the neuroblast of chortophaga viridifasciata

1985

View full text Add to dashboard Cite

Mitomycin C (MMC) induces acentric chromosome fragments in the neuroblast (Nb) of the grasshopper embryo (Chortophaga viridifasciata) after acute and chronic exposure to concentrations ranging from 10(-8) to 10(-4) M, the dose response being essentially linear up to 10(-5) M. Because Colcemid is not used in the Nb assay, it was possible to detect two additional effects of MMC: (1) Prolonged retardation of many cells occurs when they reach very late prophase; the chromosomes continue condensing and lose their orderly prophase orientation, and the nuclear envelope becomes increasingly fragile. Such cells, which were observed after both acute and chronic exposure, give the false impression of being c-metaphases when they are fixed and squashed. The frequency of retarded very late prophases and the duration of retardation are related to MMC concentration and time of exposure. A rationale is presented supporting the idea that the events associated with retarded very late prophase result from MMC effects on the nuclear envelope. (2) MMC significantly increases the frequency of Nb's with attenuated centromeres at the beginning of early anaphase, an effect that appears to be caused by a delay in the repulsion of sister chromatids that usually occurs immediately after centromere separation begins.

show abstract

Mitomycin C effects on cell cycle progression, including inhibition of very late prophase, as seen in living neuroblasts of Chortophaga viridifasciata, with some observations on mitomycin C purity☆

Cited by 8 publications

References 18 publications

Evaluation the Susceptibility of Metformin Drug and Pollen grain of Typha domengensis (Typha) on Prevention of Damage in octa4 gene Which Induced by Mitomycin c in Some Organs in Male Albino Rats

Evaluation the Susceptibility of Metformin Drug and Pollen grain of Typha domengensis (Typha) on Prevention of Damage in octa4 gene Which Induced by Mitomycin c in Some Organs in Male Albino Rats

Reversible inhibition of mammalian tubulin assembly in vitro and effects in Saccharomyces cerevisiae D61.M by mitomycin C

Chromosome fragments and other abnormalities induced by mitomycin C in the neuroblast of chortophaga viridifasciata

Contact Info

Product

Resources

About