Mitogenic and protective activity associated with a lipopolysaccharide from <i>Coxiella burnetii</i>

Paquet, Andrew; Rael, Eppie D.; Klassen, D A; Martinez, Irene; Baca, O G

doi:10.1139/m78-259

Cited by 8 publications

(4 citation statements)

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“…Recently we reported that exposure of the lysate to LPS I results in gelation (Paquet et al 1978). When the limulus lysate was exposed to subnanogram amounts of LPS 11, it too induced gelation (Fig.…”

Section: Limulus Lysate Activity Of Lps 11mentioning

confidence: 86%

Isolation and partial characterization of a lipopolysaccharide from phase II Coxiella burnetii

Baca¹,

Martinez²,

Aragon³

et al. 1980

Can. J. Microbiol.

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1980.Isolation and partial characterization of a lipopolysaccharide from phase I1 Coxiella burnetti. Can. J . Microbiol. 26: 819-826. A lipopolysaccharide (LPS) was isolated by hot phenol-water extraction from phase I1 Coxielln burtietii. The LPS was isolated from organisms that had a history of 95 serial yolk sac passages and others that were cloned. The chemical composition of the LPS was partially determined and compared with that of LPS previously isolated from phase I organisms. Most of the sugars present in phase I LPS were detected in phase I1 LPS; however, there were some quantitative and qualitative differences. The fatty acid profiles of phase I and I1 LPS were identical. The amount of LPS extracted from phase I1 organisms was about A h the amount extracted from phase I C. hirrnc,tii. Limulus lysate gelation was observed with subnanogram amounts of both phase I and I1 LPS. In complement fixation tests, both LPSs reacted only with antisera containing antibodies versus phase I antigen. Ouchterlony immunodiffusion tests demonstrated immunological identity of the two LPSs. BACA, OSWALD G.. IRENE L. MARTINEZ, ADAM S. ARAGON et DAVID KLASSEN. 1980. Isolation and partial characterization of a lipopolysaccharide from phase I1 CoxieNa burne~ii. Can. J . Microbiol. 26: 819-826. Un lipopolysaccharide (LPS) a Cte isole de Coxiella burrretii en phase I1 par une methode d'extraction phenol-eau a chaud. Ce LPS a e t t isole d'organismes qui Ctaient clones et d'organismes qui en etaient a leur quatre-vingt-cinquieme passage au niveau du sac vitellin. La composition chimique du LPS a e t e partiellement determinee et comparee a celle d'un LPS isole d'organismes en phase I. La plupart des sucres contenus dans le LPS de phase I ont etC observes dans le LPS de phase 11; cependant il y a d e s differences quantitatives et qualitatives. Les profils d'acides gras des LPS de phase I et de phase I1 sont identiques. La quantite de LPS extrait d'organismes en phase I1 est environ dix fois plus faible que la quantite extraite de C. burrletii en phase I. L'epreuve du lysat de limulus est observke avec les deux LPS de phase I et I1 a des quantites inferieures au nanogramme. Dans des epreuves de fixation du complement, les deux LPS ne reagissent qu'avec des antiserums contenant des anticorps contre l'antigkne de phase I. La technique d'immunodiffusion d'ouchterlony a demontre une identite immunologique des deux LPS.[Traduit par le journal]

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Section: Limulus Lysate Activity Of Lps 11mentioning

confidence: 86%

Isolation and partial characterization of a lipopolysaccharide from phase II Coxiella burnetii

Baca¹,

Martinez²,

Aragon³

et al. 1980

Can. J. Microbiol.

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“…Phase II LPS was extracted from cloned as well as noncloned phase II cells which had been serially passaged 95 times through embryonated eggs. Complement fixation and immunodiffusion tests indicated immunological identity of both LPS species (152). Characteristic of other bacterial LPS, phase I and II LPS caused gelation of Limulus lysates; phase I LPS induced DNA synthesis in guinea pig leukocytes, nonspecific resistance in mice to virulent Candida albicans, and the dermal Schwartzman reaction (152,176).…”

Section: Phase Variationmentioning

confidence: 98%

“…Complement fixation and immunodiffusion tests indicated immunological identity of both LPS species (152). Characteristic of other bacterial LPS, phase I and II LPS caused gelation of Limulus lysates; phase I LPS induced DNA synthesis in guinea pig leukocytes, nonspecific resistance in mice to virulent Candida albicans, and the dermal Schwartzman reaction (152,176). Thirteen of the 14 sugars found in phase I LPS were also present in phase II LPS, although some of the sugars were present in different proportions (i.e., glucose comprised 20% of the total phase I neutral sugars, but only 2 to 3% of the phase II neutral sugars).…”

Section: Phase Variationmentioning

confidence: 98%

Q fever and Coxiella burnetii: a model for host-parasite interactions.

Baca¹,

Paretsky²

1983

Microbiological Reviews

209

154

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“…Although the connection is highly speculative, the observed placental lesions are reminiscent of changes described as a local Schwartzman-like reaction following administration of endotoxin (13,17,29,51). Some biological activities associated with gram-negative bacterial endotoxin are elicited by C. burnetii lipopolysaccharide (LPS) (5), but compared with other pathogens, Coxiella LPS can be classified as a poor endotoxin (2,16,33,42,43), and large quantities of purified C. burnetii LPS are necessary to induce endotoxic changes (31). Many biological activities once attributed directly to LPS are now known to be mediated by LPS-induced cytokines.…”

Section: *~~~~Tmentioning

confidence: 99%

Histological and immunocytochemical characterization of Coxiella burnetii-associated lesions in the murine uterus and placenta

Baumgärtner

Bachmann

1992

Infect Immun

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The fetoplacental units and the postgravid uterus of BALB/cJ (H-2d) mice inoculated intraperitoneally with Coxiella burnetii (Nine Mile isolate, phase I) on day 6 of pregnancy were examined histologically and immunocytochemically at 1 to 160 days postinoculation. Clinically, abortions, stillbirths, and perinatal deaths were observed. Histological lesions in the placenta were characterized by severe necrosis of the decidua basalis and the labyrinth, fibrinoid degeneration of decidual vessels, and microthrombosis. Pyometra and endometritis at the sites of previous placental attachment, characterized by ulceration, central necrosis, and moderate cellular infiltration consisting of neutrophils and macrophages, were observed postpartum. Pups sacrificed at the age of 9 days exhibited interstitial pneumonia with few granulomas and granulomatous hepatitis and splenitis. Immunocytochemically, antigen-bearing cells were first detected in the decidua 9 days postconception, and single immunopositive cells were detected in the fetal placenta 4 days later. Thereafter, until abortion or parturition, abundant accumulation of C. burnetii antigen was observed in the maternal and fetal compartments of the placenta. Up to 28 days postinoculation, many immunopositive cells were demonstrated at the sites of previous placental attachment, whereas the adjacent endometrium contained only a few antigen-positive cells. C. burnetii antigen was demonstrated in decidual cells, trophoblasts, and macrophages and extracellularly within the sinuses of the labyrinth and in the uterine lumen but not in granulated metrial gland cells. Fetuses in utero and aborted, stillborn, or perinatally dying offspring were immunocytochemically negative for C. burnetii antigen; however, pups killed 9 days after birth showed lesion-associated positive immunoreaction in the lung, liver, and spleen. The present study shows that infection with C. burnetii during pregnancy results in uncontrolled growth of the organism in the murine uteroplacental unit and that associated lesions are characterized by necrosis of placental tissues, fibrinoid degeneration of decidual vessels, and microthrombosis.Coxiella burnetii, the etiological agent of Q fever, is an obligate, gram-variable, intracellular pathogen of the family Rickettsiaceae (49). Infection with C. bumetii has been demonstrated in arthropods, numerous free-living and domestic animal species, and humans (1,40). In general, 5232 on August 5, 2020 by guest http://iai.asm.org/ Downloaded from 50. Wiesmann, E., and F. Busrki. 1955. Die veterinar-medizinische Bedeutung der Rickettsia-bumeti-Infektionen bei Ziege, Schaf und Rind in der Schweiz. Schweiz. Arch. Tierheilkd. 97:569-574. 51. Zaht, P. A., and C. Bjerknes. 1943. Induction of deciduaplacental hemorrhage in mice by the endotoxins of certain gram-negative bacteria.

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Mitogenic and protective activity associated with a lipopolysaccharide from Coxiella burnetii

Cited by 8 publications

References 0 publications

Isolation and partial characterization of a lipopolysaccharide from phase II Coxiella burnetii

Isolation and partial characterization of a lipopolysaccharide from phase II Coxiella burnetii

Q fever and Coxiella burnetii: a model for host-parasite interactions.

Histological and immunocytochemical characterization of Coxiella burnetii-associated lesions in the murine uterus and placenta

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