Mitogen-induced disorganization of capillary-like structures formed by human large vessel endothelial cells in vitro

Allikmets, E. Yu.; Danilov, Sergei M.

doi:10.1016/0040-8166(86)90014-5

Cited by 28 publications

(9 citation statements)

References 14 publications

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Section: Vascularizationsupporting

confidence: 81%

“…cultures was not expected since previous experiments had shown that this transformation requires very long periods of time, especially when monolayers are provided with endothelial cell growth factor. When attempts were made to duplicate the in vitro vascularization observed by Maciag using H.U.V.E.C.s in the absence of any fields, 1-2 months was required before tube formation was observed in the culture dishes (data not shown), a time-course consistent with other researcher's observations (Maciag et al, 1982;Allikmets and Danilov, 1986). For the vascularization experiments in the field, H.U.V.E.C.s were passaged at high density (1:l) or at densities that allowed cell replication (1:2) with little change in the outcomes of the experiments, indicating that cell proliferation played a limited role in electromagnetic-field-stimulated in vitro vascularization.…”

Section: Vascularizationsupporting

confidence: 57%

“…5D). The circumference of the vessel lumen was formed by one to three cells and the lumen itself contained amorphous debris as described in other in vitro vascularization studies (Maciag et al, 1982;Allikmets and Danilov, 1986). The integrity of the tubular structures was maintained by darkly staining regions in adjacent plasma membranes which may be junctional complexes (Fig.…”

Section: Vascularizationmentioning

confidence: 97%

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Endothelial cell response to pulsed electromagnetic fields: Stimulation of growth rate and angiogenesis in vitro

Yen-Patton

Patton

Beer

et al. 1988

Journal Cellular Physiology

153

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The effects of pulsed electromagnetic fields on the repopulation rate of denuded regions of endothelial cell monolayers and on endothelial cell reorganization into complex vessellike structures was monitored in vitro by using human umbilical vein and bovine aortic endothelial cells. A small (20-40%) but statistically significant enhancement in growth rate of partially denuded endothelial cell monolayers as determined by tritiated thymidine incorporation was observed in the presence of pulsed electromagnetic fields. Morphologically, endothelial cells entering the denuded regions were observed to be elongated, often connecting end to end to form a mycelial or "sprouting" pattern when exposed to pulsed electromagnetic fields. This was in contrast to cells outside of the field which had a more cuboidal morphology. Complete disruption of the endothelial cell monolayer by passaging the cells with EDTA-trypsin resulted in reorganization of some of the cells into three-dimensional vessellike structures after as little as 5-8 hours in the presence of the pulsed electromagnetic field. This reorganization occurred in the presence of heparin, endothelial cell growth factor, and a competent fibronectin matrix. Vascularization for comparable cultures outside of the field did not occur during the time-course of the experiments. Discrete stages of neovascularization were observed in the presence of the field that were qualitatively similar to stages of angiogenesis observed in vivo.

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Section: Vascularizationsupporting

confidence: 81%

Section: Vascularizationsupporting

confidence: 57%

Section: Vascularizationmentioning

confidence: 97%

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Endothelial cell response to pulsed electromagnetic fields: Stimulation of growth rate and angiogenesis in vitro

Yen-Patton

Patton

Beer

et al. 1988

Journal Cellular Physiology

153

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“…Although the responsiveness of HPMECs could be modulated by endothelial growth factors such as VEGF, endothelial cell growth factor (ECGF), or bFGF, the basic patterns were shown in serumfree media and were determined by ECM composition. This dominant ECM signaling supports evidence that whereas angiogenic growth factors promote spreading and proliferation, ECM proteins regulate endothelial differentiation (Allikmets and Danilov 1986). There is also in vivo evidence that exposure of endothelial cells to ECM components through disruption of the basement membrane stimulates angiogenic remodeling (Bernfield et al 1984;Zhou et al 1998), and this is independent of the hypoxia-VEGF pathway (Hansen Smith et al 1996).…”

Section: Discussionmentioning

confidence: 75%

Distinct Patterns of Microvascular Endothelial Cell Morphology Are Determined by Extracellular Matrix Composition

et al. 2004

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Endothelial interactions with the extracellular matrix (ECM) play important roles in angiogenesis but whether specific ECM signals can determine specific cellular morphologies is unclear. The authors compared in vitro ECM-induced morphological responses of the phenotypically distinct human placental microvascular endothelial cells (HPMECs) with large vessel endothelial cells (HUVECs). HPMECs showed distinct patterns of reorganization in response to collagen-I or collagen-IV (monolayer disruption, sprouting, migration) and Matrigel or laminin-A (intussusception, cord formation, tubulogenesis), and an intermediate response to fibrin; whereas HUVECs responded similarly to collagen-1 and Matrigel (elongation, lattice formation, vacuolation) and showed little response to fibrin. Although the extent of collagen and Matrigel responses of HPMECs were increased by serum, acidic or basic fibroblast growth factor (aFGF, bFGF), or vascular endothelial growth factor (VEGF), and varied with matrix protein concentration, the basic patterns were matrix specific, and were independent of fibronectin. The collagen responses correlated with disruption of adherens and tight junctions and the formation of filopodial protrusions. Matrigel responses were associated with up-regulated junctional localization of VE-cadherin, and tubulogenesis developed mainly through paracellular remodeling rather than intracellular vacuolation. Overall, these findings suggest that distinct ECM interactions stimulate specific morphological responses. These signals may regulate morphological behaviour in the angiogenesis cycle, switching endothelial cells between migratory and vasculogenic phenotypes.

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“…Endothelial cells were isolated from human umbilical veins and cultured as described elsewhere in detail (Allikmets & Danilov, 1986;Resink et al, 1987). Cells from passages 1 to 7 were harvested by use of trypsin-EDTA and seeded on gelatin-coated glass cover slips in 35mm plastic Petri dishes.…”

Section: Properties Ofunstimulated Cellsmentioning

confidence: 99%

Histamine‐induced inward currents in cultured endothelial cells from human umbilical vein

Bregestovski

Bakhramov

Danilov

et al. 1988

British J Pharmacology

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1 The membrane response to applied histamine of cultured endothelial cells from human umbilical vein was studied by use of whole cell and single channel patch clamp techniques. A value of -27 + 1.4 mV was found for the resting potential under whole cell current clamp. No voltage-gated currents were seen at either the macroscopic or single channel levels. 2 At holding potentials of -20 to -40 mV, histamine evoked slow rising, long lasting whole cell inward currents. The inward current was associated with depolarization and decreased input resistance. The calcium ionophore A23187 provoked similar whole cell inward currents. 3 Single channel currents were observed in cell-attached and inside-out patches for both histamine and A23187. The single channel conductance was about 20 pS with a mean open time of 5 ms and a reversal potential of OmV in symmetrical potassium solutions. Internal sodium blocked outward going currents. 4 For cell-attached patches, histamine-dependent channel activity required external calcium and was also seen when histamine was present in the bath but not the pipette. Recording from insideout patches revealed that decreases in 'internal' calcium resulted in the disappearance of channel activity. 5 The histamine-dependent inward current appears to involve calcium-dependent activation of cationic channels.

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Mitogen-induced disorganization of capillary-like structures formed by human large vessel endothelial cells in vitro

Cited by 28 publications

References 14 publications

Endothelial cell response to pulsed electromagnetic fields: Stimulation of growth rate and angiogenesis in vitro

Endothelial cell response to pulsed electromagnetic fields: Stimulation of growth rate and angiogenesis in vitro

Distinct Patterns of Microvascular Endothelial Cell Morphology Are Determined by Extracellular Matrix Composition

Histamine‐induced inward currents in cultured endothelial cells from human umbilical vein

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