Mitogen-activated protein kinase 6 negatively regulates secondary wall biosynthesis by modulating MYB46 protein stability in Arabidopsis thaliana

Im, Jong Hee; Ko, Jae‐Heung; Kim, Wonchan; Crain, Brent A.; Keathley, Daniel E.; Han, Kyung-Hwan

doi:10.1371/journal.pgen.1009510

Cited by 16 publications

(19 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As components of complexes, MAPKs and MYBs participate in different biological development processes in plants. Mitogen-activated protein kinase 6 negatively regulates SCW biosynthesis by modulating MYB46 protein stability in Arabidopsis ( Im et al, 2021 ). MYB75 phosphorylation by MPK4 is required for light-induced anthocyanin accumulation in Arabidopsis ( Li et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

RLM1, Encoding an R2R3 MYB Transcription Factor, Regulates the Development of Secondary Cell Wall in Rice

et al. 2022

View full text Add to dashboard Cite

Leaf morphology is an important component of rice ideal plant type. To date, many regulatory genes influencing leaf morphology in rice have been cloned, and their underlying molecular regulatory mechanism has been preliminarily clarified. However, the fine regulation relationship of leaf morphogenesis and plant type remains largely elusive. In this study, a rolling-leaf mutant, named rlm1-D, was obtained and controlled by a pair of dominant nuclear genes. Cytological observations revealed that the rlm1 was mainly caused by abnormal deposition of secondary cell walls. Molecular evidence showed ectopic expression of a MYB-type transcription factor LOC_Os05g46610 was responsible for the phenotype of rlm1-D. A series of experiments, including the transcription factor-centered technology, DNA-binding assay, and electrophoretic mobility shift assay, verified that RLM1 can bind to the promoter of OsCAD2, a key gene responsible for lignin biosynthesis in rice. An interacting partner of RLM1, OsMAPK10, was identified. Multiple biochemical assays confirmed that OsMAPK10 interacted with RLM1. OsMAPK10 positively regulated the lignin content in the leaves and stems of rice. Moreover, OsMAPK10 contributes to RLM1 activation of downstream target genes. In particular, RLM1 is exclusively expressed in the stems at the mature plant stage. The yield of RLM1 knockdown lines increased by over 11% without other adverse agricultural trait penalties, indicating great practical application value. A MAPK-MYB-OsCAD2 genetic regulatory network controlling SCW was proposed, providing a theoretical significance and practical value for shaping the ideal plant type and improving rice yield.

show abstract

Section: Discussionmentioning

confidence: 99%

RLM1, Encoding an R2R3 MYB Transcription Factor, Regulates the Development of Secondary Cell Wall in Rice

et al. 2022

View full text Add to dashboard Cite

show abstract

“…In Populus , the phosphorylation of PdLTF1 (an MYB transcription factor) by PdMPK6 is the key to regulate lignin accumulation in response to environmental stimuli (Gui et al, 2019). Secondary cell wall biosynthesis is regulated by MPK6 phosphorylation and the degradation of MYB46 in Arabidopsis (Im et al, 2021). Therefore, a MYB4a degradation pathway in plants may alleviate the inhibition of the CsMYB4a-CsIAA4 complex.…”

Section: Discussionmentioning

confidence: 99%

CsMYB4a from Camellia sinensis Regulates the Auxin Signaling Pathway by Interacting with CsIAA4

Jiang

et al. 2021

Preprint

View full text Add to dashboard Cite

Members of the R2R3-MYB4 subgroup are well-known negative regulatory transcription factors of phenylpropane and lignin pathways. In this study, we found that transgenic tobacco plants overexpressing a R2R3-MYB4 subgroup gene from Camellia sinensis (CsMYB4a) showed inhibited growth that was not regulated by phenylpropane and lignin pathways, and these plants exhibited altered sensitivity to synthetic auxin 1-naphthaleneacetic acid (α-NAA) treatment. An auxin/indole-3-acetic acid 4 (AUX/IAA4) gene from Camellia sinensis (CsIAA4) participating in the regulation of the auxin signal transduction pathway was screened from the yeast two-hybrid library with CsMYB4a as the bait protein, and tobacco plants overexpressing this gene showed a series of auxin-deficiency phenotypes, such as dwarfism, small leaves, reduced lateral roots, and a shorter primary root. CsIAA4 transgenic tobacco plants were less sensitive to exogenous α-NAA than control plants, which was consistent with the findings for CsMYB4a transgenic tobacco plants. The knockout of the endogenous NtIAA4 gene (a CsIAA4 homologous gene) in tobacco plants alleviated growth inhibition in CsMYB4a transgenic tobacco plants. Furthermore, protein-protein interaction experiments proved that domain II of CsIAA4 is the key motif for the interaction between CsIAA4 and CsMYB4a and that the degradation of CsIAA4 is prevented when CsMYB4a interacts with CsIAA4. In summary, our results suggest that CsMYB4a is a multifunctional transcription factor that regulates the auxin signaling pathway, phenylpropane and lignin pathways. This study provides new insights into the multiple functions of R2R3-MYB4 subgroup members as a group of well-known negative regulatory transcription factors.

show abstract

“…GUS conjugated MYB46 promoter was used as previously reported (Jong Hee Im et al, 2021). For generation of MYC2 overexpressing plant, cDNA of MYC2 was cloned into pDONR/Zeo (Thermo Fisher Scientific) and LR reaction was carried out with pEarleygate201.…”

Section: Plasmid Generationmentioning

confidence: 99%

“…This construct was introduced into Agrobacterium tumefaciens (GV3101) and transformed Arabidopsis with floral dip method. For transient promoter assay, MYC2 , MYC3 andMYC4 were cloned into HBT vector (Im et al, 2021) with YFP conjugation.…”

Section: Plasmid Generationmentioning

confidence: 99%

See 1 more Smart Citation

Jasmonate activates secondary cell wall biosynthesis through MYC2-MYB46 module

Han

Son

et al. 2023

Preprint

View full text Add to dashboard Cite

The formation of secondary cell walls is tightly regulated spatio-temporally by various developmental and environmental signals. Successful fine-tuning of the trade-off between secondary cell wall biosynthesis and stress responses requires better understanding of how plant growth is regulated under environmental stress conditions. However, current understanding of the interplay between environmental signaling and secondary cell wall formation is limited. The lipid-derived plant hormone jasmonate (JA) and its derivatives are important signaling components involved in various physiological processes including plant growth, development, and abiotic/biotic stress response. Recent studies suggest that JA may be involved in secondary cell wall formation. We tested this hypothesis using the transcription factor MYB46, a master switch for secondary wall biosynthesis, and JA treatments. Both the transcripts and protein levels of MYB46 were significantly increased by the JA treatments, which also triggered the upregulation of MYB46 downstream genes with increased secondary wall formation. We then show that this JA-induced upregulation of MYB46 function was mediated by MYC2, a basic helix-loop-helix (bHLH) domain–containing transcription factor, which plays a pivotal role in the JA-mediated changes. We conclude that this MYC2-MYB46 module is a key component of the plant response to JA signaling.

show abstract

Mitogen-activated protein kinase 6 negatively regulates secondary wall biosynthesis by modulating MYB46 protein stability in Arabidopsis thaliana

Cited by 16 publications

References 36 publications

RLM1, Encoding an R2R3 MYB Transcription Factor, Regulates the Development of Secondary Cell Wall in Rice

RLM1, Encoding an R2R3 MYB Transcription Factor, Regulates the Development of Secondary Cell Wall in Rice

CsMYB4a from Camellia sinensis Regulates the Auxin Signaling Pathway by Interacting with CsIAA4

Jasmonate activates secondary cell wall biosynthesis through MYC2-MYB46 module

Contact Info

Product

Resources

About