Mitochondrial regulation of insect cell apoptosis: Evidence for permeability transition pore-independent cytochrome-c release in the Lepidopteran Sf9 cells

Kumarswamy, Regalla; Seth, Rakesh; Dwarakanath, Bilikere S.; Chandna, Sudhir

doi:10.1016/j.biocel.2008.12.009

Cited by 64 publications

(51 citation statements)

References 60 publications

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“…25 Moreover, a series of biochemical experiments determine that Cyt C is required for lepidopteran apoptosis at an early stage and suggest that the chemical induction of TP53/p53-Bcl-2-Bax-Cyt C signaling is indispensable for apoptosis in lepidopteran cells. 24,26 In Drosophila and the silkworm, Bombyx mori, the molting hormone (20-hydroxyecdysone, 20E) is both necessary and sufficient to induce autophagy and apoptosis during metamorphosis. 8,27,28 20E and its receptor complex (20E-EcR-USP) trigger the transcriptional cascade of 20E primary-response genes (including Br-C, E74, E75 and E93) and thus 20E secondaryresponse genes.…”

Section: Basic Research Papermentioning

confidence: 99%

BmATG5 and BmATG6 mediate apoptosis following autophagy induced by 20-hydroxyecdysone or starvation

et al. 2016

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Autophagy and apoptosis, which could be induced by common stimuli, play crucial roles in development and disease. The functional relationship between autophagy and apoptosis is complex, due to the dual effects of autophagy. In the Bombyx Bm-12 cells, 20-hydroxyecdysone (20E) treatment or starvationinduced cell death, with autophagy preceding apoptosis. In response to 20E or starvation, BmATG8 was rapidly cleaved and conjugated with PE to form BmATG8-PE; subsequently, BmATG5 and BmATG6 were cleaved into BmATG5-tN and BmATG6-C, respectively. Reduction of expression of BmAtg5 or BmAtg6 by RNAi decreased the proportion of cells undergoing both autophagy and apoptosis after 20E treatment or starvation. Overexpression of BmAtg5 or BmAtg6 induced autophagy but not apoptosis in the absence of the stimuli, but promoted both autophagy and apoptosis induced by 20E or starvation. Notably, overexpression of cleavage site-deleted BmAtg5 or BmAtg6 increased autophagy but not apoptosis induced by 20E or starvation, whereas overexpression of BmAtg5-tN and BmAtg6-C was able to directly trigger apoptosis or promote the induced apoptosis. In conclusion, being cleaved into BmATG5-tN and BmATG6-C, BmATG5 and BmATG6 mediate apoptosis following autophagy induced by 20E or starvation in Bombyx Bm-12 cells, reflecting that autophagy precedes apoptosis in the midgut during Bombyx metamorphosis.

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Section: Basic Research Papermentioning

confidence: 99%

BmATG5 and BmATG6 mediate apoptosis following autophagy induced by 20-hydroxyecdysone or starvation

et al. 2016

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“…We used Sf9 insect cells that display classical apoptotic morphology when treated with apoptogenic agents such as Actinomycin-D (a transcriptional inhibitor), Etoposide (topoisomerase inhibitor) and ionizing radiation Suman et al 2009;Kumarswamy et al 2009). In order to demonstrate the advantages of this new method, we validated the results with conventional phenol-chloroform method, and monitored morphological apoptotic alterations using the differential image contrast (DIC) microscopy.…”

Section: Introductionmentioning

confidence: 99%

An improved non-enzymatic “DNA ladder assay” for more sensitive and early detection of apoptosis

2011

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Conventional DNA ladder assay has certain shortcomings such as loss of DNA fragments during sample processing, involvement of multiple steps and requirement of expensive reagents. The present study demonstrates a rapid, easy-to-perform cost-effective method for detection of apoptotic DNA fragments with considerable improvement in the sensitivity by avoiding loss of DNA fragments. It involves a few minutes of procedure involving direct lysis of cells with dimethyl sulphoxide (DMSO), brief vortexing, addition of 2% SDS-TE buffer, and a single step of centrifugation. This cost-and time-efficient method reduces the assay time considerably and can be used for a large number of samples with excellent sensitivity.

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“…Mitochondrial membrane potential (ΔΨm) is a marker of mitochondrial function often associated with apoptosis 13,14 . results indicated a remarkable decrease in ΔΨm ( Figure 6).…”

Section: Mitochondrial Membrane Potential (δψM) Changementioning

confidence: 99%

Apoptotic effects of 1,5-bis-(5-nitro-2-furanyl)-1, K]4-pentadien-3-one on Drosophila SL2 cells

Ren

Jin

Tao

et al. 2015

Mol. Cell. Toxicol.

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in recent years, concerns over the potential impact of synthetic pesticides on the environment have promoted the research and development of environmentally friendly "green" pesticides. in the current study, we utilized a green biomimetic insecticide, 1,5-bis-(5-nitro-2-furanyl)-1,4-pentadien-3-one (compound C), to examine its cytotoxicity on an insect cell line, Drosophila melanogaster (Sl2). results from MTT assay showed cells treated with 100 μM of compound C for 48 h significantly inhibited the growth of Sl2 cells by 87.71±0.96%. We subsequently attempted to illustrate the act on mechanism of compound C at the cellular level and found that it initiated apoptosis through a mitochondrial-dependent mechanism that increased the activity of caspase-3 and altered the cell cycle. These results suggest that the green biomimetic insecticide, compound C, is a novel and potent inducer of insect cell apoptosis.

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Mitochondrial regulation of insect cell apoptosis: Evidence for permeability transition pore-independent cytochrome-c release in the Lepidopteran Sf9 cells

Cited by 64 publications

References 60 publications

BmATG5 and BmATG6 mediate apoptosis following autophagy induced by 20-hydroxyecdysone or starvation

BmATG5 and BmATG6 mediate apoptosis following autophagy induced by 20-hydroxyecdysone or starvation

An improved non-enzymatic “DNA ladder assay” for more sensitive and early detection of apoptosis

Apoptotic effects of 1,5-bis-(5-nitro-2-furanyl)-1, K]4-pentadien-3-one on Drosophila SL2 cells

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