Mitochondrial proteomics reveals new insights into embryogenic competence acquisition in Carica papaya L. callus

Vale, Ellen Moura; Santana, Diederson Bortolini; Reis, Robert L.; Sousa, Kariane Rodrigues de; Filho, Gonçalo Apolinário de Souza; Oliveira, Jurandi Gonçalves de; Santa–Catarina, Claudete; Silveira, Vanildo

doi:10.1016/j.jprot.2021.104434

Cited by 3 publications

(4 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the contrary, when the content of Spm was low, the growth and development of embryos was also inhibited and aborted gradually in this environment. In recent years, the same results were obtained in the studies on the dynamic changes of endogenous PAs during somatic embryogenesis in other plants ( Shoeb et al., 2001 ; Vale et al., 2022 ).…”

Section: Discussionsupporting

confidence: 72%

Study on the relationship between the changes in polyamine content and seedless grapes embryo rescue breeding

Li,

Han

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

This study was envisaged to investigate the physiological reasons affecting the embryo development and abortion of seedless grapes on the basis of the previous embryo rescue breeding techniques of seedless grapes. Specifically, the relationship between the embryo rescue breeding of seedless grapes and the change of polyamine content was evaluated, in order to provide hybrid germplasm in the breeding of new seedless grape cultivars. Four ovules of 4 naturally pollinated Eurasian seedless grape cultivars, including ‘Thompson Seedless’ grape (hereinafter referred to as ‘Seedless White’ grape), ‘Flame Seedless’ grape, ‘Heshi Seedless’ grape and ‘Ruby Seedless’ grape were employed for the study. Changes in the endogenous polyamine content, exogenous polyamine content, and the suitable combination of exogenous polyamines in the seedless grape berries and isolated ovules were determined during the best embryo rescue period. Furthermore, the effect of different exogenous polyamine contents on the germination and seedling rate of different seedless grape embryos was analyzed. In the best embryo rescue period, the number of ovules had different effects on the content of polyamines. For seedless grape cultivars with 4 ovules, a high content of polyamines was found to be more beneficial in the embryonic development. The existence of embryos had different effects on the development of embryos. In the ovules with embryo, an increase in the content of polyamine was beneficial to the growth and development of the ovule. Different ratios of exogenous polyamines had varying effects on the embryonic development. Putrescine (Put) exhibited the greatest effect on the embryonic development. Further, correlation analysis showed that different combinations of exogenous polyamines had varying effects on the embryonic development. A maximal ovule development was observed in the combination of exogenous polyamines of putrescine2+spermidine2+spermine1. For maximal embryo germination and seeding formation, the optimal combination was putrescine2+spermidine2+spermine2. Irrespective to the number of ovules or the existence of embryos, the results indicated that a high content of endogenous polyamines promoted the growth and development of embryos. The embryo rescue efficiency of different exogenous polyamines was different, and the appropriate combination of exogenous polyamines was beneficial to the growth and development of ovules, with a high development rate of the ovule and seedling.

show abstract

Section: Discussionsupporting

confidence: 72%

Study on the relationship between the changes in polyamine content and seedless grapes embryo rescue breeding

Li,

Han

et al. 2024

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…These proteins are involved in indole-3-acetic acid (IAA) conjugation and can regulate free auxin levels, as well as proteins involved in ethylene responses. Auxin homeostasis is an important factor in the embryogenic competence of C. papaya, where the accumulation of GH3 proteins has already been associated with the differentiation of somatic embryos [11]. In the present study, a probable indole-3-acetic acid-amido synthetase GH3.1 (evm.model.supercontig_292.1) and two GH3.6 (evm.model.supercontig_1065.2 and evm.TU.contig_32826.1, orthologs of AtDFL1) accumulated during the induction of embryogenic callus (Table 1) (Figure 4).…”

Section: Proteins Involved In Hormone Responses Are Induced Early Aft...mentioning

confidence: 61%

“…Modulation of embryogenic competence in C. papaya callus involves the accumulation of energy metabolism-related proteins [11]. In this regard, proteins with a role in electrochemical gradient generation and proton transport were identified, as well as pyrophosphatases regulated throughout callus induction (Table 1).…”

Section: The Explant Contains Storage Reserves That Are Consumed Duri...mentioning

confidence: 99%

“…Another study identified 801 proteins at different stages of somatic embryo development, showing specific proteomic profiles for each stage [10]. Furthermore, the study of the mitochondrial proteome of callus with contrasting embryogenic competence led to the identification of 237 proteins and contributed to the discovery of proteins important for energy production and auxin homeostasis [11]. On the other hand, these studies used already differentiated callus for proteomic analyses, where the process of induction and formation of embryogenic callus is still poorly understood.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Time-Dependent Proteomic Signatures Associated with Embryogenic Callus Induction in Carica papaya L.

Xavier,

Corrêa,

da Paschoa

et al. 2023

Plants

Self Cite

View full text Add to dashboard Cite

Sex segregation increases the cost of Carica papaya production through seed-based propagation. Therefore, in vitro techniques are an attractive option for clonal propagation, especially of hermaphroditic plants. Here, we performed a temporal analysis of the proteome of C. papaya calli aiming to identify the key players involved in embryogenic callus formation. Mature zygotic embryos used as explants were treated with 20 μM 2,4-dichlorophenoxyacetic acid to induce embryogenic callus. Total proteins were extracted from explants at 0 (zygotic embryo) and after 7, 14, and 21 days of induction. A total of 1407 proteins were identified using a bottom-up proteomic approach. The clustering analysis revealed four distinct patterns of protein accumulation throughout callus induction. Proteins related to seed maturation and storage are abundant in the explant before induction, decreasing as callus formation progresses. Carbohydrate and amino acid metabolisms, aerobic respiration, and protein catabolic processes were enriched throughout days of callus induction. Protein kinases associated with auxin responses, such as SKP1-like proteins 1B, accumulated in response to callus induction. Additionally, regulatory proteins, including histone deacetylase (HD2C) and argonaute 1 (AGO1), were more abundant at 7 days, suggesting their role in the acquisition of embryogenic competence. Predicted protein–protein networks revealed the regulatory role of proteins 14-3-3 accumulated during callus induction and the association of proteins involved in oxidative phosphorylation and hormone response. Our findings emphasize the modulation of the proteome during embryogenic callus initiation and identify regulatory proteins that might be involved in the activation of this process.

show abstract

Loss of APOO (MIC26) aggravates obesity-related whitening of brown adipose tissue via PPARα-mediated functional interplay between mitochondria and peroxisomes

Guo

et al. 2023

Metabolism

View full text Add to dashboard Cite

Mitochondrial proteomics reveals new insights into embryogenic competence acquisition in Carica papaya L. callus

Cited by 3 publications

References 72 publications

Study on the relationship between the changes in polyamine content and seedless grapes embryo rescue breeding

Study on the relationship between the changes in polyamine content and seedless grapes embryo rescue breeding

Time-Dependent Proteomic Signatures Associated with Embryogenic Callus Induction in Carica papaya L.

Loss of APOO (MIC26) aggravates obesity-related whitening of brown adipose tissue via PPARα-mediated functional interplay between mitochondria and peroxisomes

Contact Info

Product

Resources

About